Effect of Fixatives and Fixation Period on Morphology and Immunohistochemistry of Feline Ovarian Tissue
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Reagents and Chemicals
2.2. Ovary Transport and Preparation of Ovarian Fragments
2.3. Experimental Design
2.4. Histology
Image Analysis (Hematoxylin and Eosin)
2.5. Immunohistochemistry
Image Analysis (Immunohistochemistry)
2.6. Statistical Analysis
3. Results
3.1. Histology
3.2. Immunohistochemistry
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Follicle | Description |
---|---|
Grade 1 | Follicle is spherical in shape, evenly distributed follicular cells, intact stroma, spherical oocyte, intact nucleus and nucleolus with homogenous cytoplasm. |
Grade 2 | Follicle is spherical in shape, evenly distributed follicular cells, intact stroma and spherical oocyte, misshapen nucleus and/or not homogenous cytoplasm. |
Grade 3 | Follicular cells pulled away from the stroma but oocyte spherical. |
Grade 4 | Follicular cells pulled away from the stroma and/or oocyte misshapen, vacuolated, pyknotic nucleus and/or disorganized granulosa cells. |
Primordial | Oocyte surrounded by flattened follicular cells. |
Transitional | Some flattened follicular cells have been converted to cuboidal cells. |
Primary | Oocyte surrounded by cuboidal follicular cells. |
Secondary | Antral space appears. |
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Alkali, I.M.; Colombo, M.; Rodak, O.; Nizanski, W.; Luvoni, G.C. Effect of Fixatives and Fixation Period on Morphology and Immunohistochemistry of Feline Ovarian Tissue. Animals 2024, 14, 825. https://doi.org/10.3390/ani14060825
Alkali IM, Colombo M, Rodak O, Nizanski W, Luvoni GC. Effect of Fixatives and Fixation Period on Morphology and Immunohistochemistry of Feline Ovarian Tissue. Animals. 2024; 14(6):825. https://doi.org/10.3390/ani14060825
Chicago/Turabian StyleAlkali, Isa Mohammed, Martina Colombo, Olga Rodak, Wojciech Nizanski, and Gaia Cecilia Luvoni. 2024. "Effect of Fixatives and Fixation Period on Morphology and Immunohistochemistry of Feline Ovarian Tissue" Animals 14, no. 6: 825. https://doi.org/10.3390/ani14060825
APA StyleAlkali, I. M., Colombo, M., Rodak, O., Nizanski, W., & Luvoni, G. C. (2024). Effect of Fixatives and Fixation Period on Morphology and Immunohistochemistry of Feline Ovarian Tissue. Animals, 14(6), 825. https://doi.org/10.3390/ani14060825