Damage to Sorubim cuspicaudus Sperm Cryopreserved with Ethylene Glycol
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals and Semen Collection Semen
2.2. Concentration and Sperm Kinetics
2.3. Semen Cryopreservation, Freezing, and Thawing
2.4. Damages to Sperm Membrane (Mem-d), Mitochondria (Mit-d), and DNA Fragmentation (DNA-f)
2.5. Fertilization and Hatching Rates
2.6. Statistical Analysis
3. Results
3.1. Seminal Plasma Osmolarity, Concentration, and Sperm Kinetics
3.2. Damages in Prefrozen and Thawed Sperm
3.3. Fertilization and Hatching Rates
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Parameters | Fresh Sperm | Prefrozen Sperm | Thawed Sperm | ||||
---|---|---|---|---|---|---|---|
6% EG | 8% EG | 10% EG | 6% EG | 8% EG | 10% EG | ||
tM (%) | 95.1 ± 2.7 a | 61.0 ± 15.3 b | 51.2 ± 10.5 b,c | 33.8 ± 11.6 d | 35.0 ± 5.7 c,d | 31.9 ± 2.1 d | 32.5 ± 5 d |
Rapid (%) | 18.6 ± 5.9 a | 0.9 ± 1.2 b | 0.3 ± 0.3 b | 0.2 ± 0.2 b | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
Medium (%) | 58.0 ± 9.9 a | 16.9 ± 7.2 b | 9.9 ± 6.5 b | 1.7 ± 0.2 c | 1.9 ± 0.6 c | 1.3 ± 0.7 c | 1.5 ± 0.5 c |
Slow (%) | 20.5 ± 5.3 b | 43.3 ± 9.6 a | 41.0 ± 5.1 a | 30.4 ± 9.4 a,b | 33.1 ± 5.2 a | 30.6 ± 1.6 a b | 31.1 ± 4.7 a b |
Static (%) | 5.5 ± 1.9 d | 39.0 ± 15.4 c | 48.8 ± 10.5 bc | 66.2 ± 11.6 a b | 65.0 ± 5.7 a,b | 68.1 ± 2.1 a | 67.9 ± 5.1 a,b |
VCL (µm/s) | 72.9 ± 5.5 a | 34.5 ± 4.2 b | 28.2 ± 5.1 b,c | 23.3 ± 3.2 c,d | 23.2 ± 2.6 c,d | 21.1 ± 1.8 d | 21.1 ± 1.3 d |
VSL (µm/s) | 55.9 ± 8.8 a | 18.5 ± 5.8 b | 13.4 ± 4.7 b,c | 7.7 ± 3.6 c | 7.0 ± 0.6 c | 6.9 ± 1.1 c | 7.1 ± 1.2 c |
Damage | Fresh Semen | Prefrozen Semen | Thawed Semen | ||||
---|---|---|---|---|---|---|---|
6% EG | 8% EG | 10% EG | 6% EG | 8% EG | 10% EG | ||
Mem-d (%) | 1.0 ± 0.5 c | 2.4 ± 1.1 bc | 3.0 ± 2.0 b,c | 4.1 ± 1.1 b | 24.8 ± 2.2 a | 24.5 ± 2.2 a | 26.6 ± 5.4 a |
Mit-d (%) | 1.4 ± 0.9 c | 16.1 ± 2 b | 16.5 ± 3.4 b | 18.3 ± 5.7 b | 49.0 ± 7.3 a | 51.4 ± 2.7 a | 47.8 ± 5.4 a |
DNA-f (%) | 2.4 ± 0.8 c | 6.7 ± 2.5 b | 6.8 ± 3.3 b | 9.5 ± 2.2 b | 44.4 ± 3.4 a | 37.9 ± 2.8 a | 38.6 ± 6.8 a |
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Atencio-García, V.; Padilla-Izquierdo, D.; Robles-González, J.; Prieto-Guevara, M.; Pardo-Carrasco, S.; Espinosa-Araujo, J. Damage to Sorubim cuspicaudus Sperm Cryopreserved with Ethylene Glycol. Animals 2023, 13, 235. https://doi.org/10.3390/ani13020235
Atencio-García V, Padilla-Izquierdo D, Robles-González J, Prieto-Guevara M, Pardo-Carrasco S, Espinosa-Araujo J. Damage to Sorubim cuspicaudus Sperm Cryopreserved with Ethylene Glycol. Animals. 2023; 13(2):235. https://doi.org/10.3390/ani13020235
Chicago/Turabian StyleAtencio-García, Víctor, Denia Padilla-Izquierdo, Juana Robles-González, Martha Prieto-Guevara, Sandra Pardo-Carrasco, and José Espinosa-Araujo. 2023. "Damage to Sorubim cuspicaudus Sperm Cryopreserved with Ethylene Glycol" Animals 13, no. 2: 235. https://doi.org/10.3390/ani13020235
APA StyleAtencio-García, V., Padilla-Izquierdo, D., Robles-González, J., Prieto-Guevara, M., Pardo-Carrasco, S., & Espinosa-Araujo, J. (2023). Damage to Sorubim cuspicaudus Sperm Cryopreserved with Ethylene Glycol. Animals, 13(2), 235. https://doi.org/10.3390/ani13020235