Natural Evolution of Porcine Epidemic Diarrhea Viruses Isolated from Maternally Immunized Piglets
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Collection of Cell Culture and Clinical Samples
2.2. Multiple Detection of Virus
2.3. Histological Analysis of the Materials
2.4. Sequence Analysis and Phylogenetic Construction
2.5. Virus Isolation and Purification
2.6. Selection of Susceptible Cells
2.7. Tissue Culture Infection Dose (TCID50) Measurement
2.8. Growth Dynamic Curve of Different Cells Infected by the Virus
2.9. Indirect Immunofluorescence Assay (IFA)
2.10. Analysis of S Gene Recombination
3. Results
3.1. PEDV Virus Screening
3.2. S, ORF3 Amino Acid Sequence Alignment and Evolutionary Analysis
3.3. Isolation and Identification of PEDV Virus
3.4. Analysis of S Gene Recombination
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Name of Sample | Time of Collection | PEDV Vaccination Status (Sows) | PEDV Positive or Not |
---|---|---|---|
HB-lf-01 | November 2019 | Inactivated vaccine CV777 | Negative |
HB-lf-02 | November 2019 | Inactivated vaccine CV777 | Negative |
HB-lf-03 | November 2019 | Inactivated vaccine CV777 | Negative |
Ll | November 2019 | Duplex live attenuated vaccine AJ1102 | Negative |
ZMD1 | November 2019 | Live attenuated triple vaccine CV777 | Negative |
ZMD2 | November 2019 | Live attenuated triple vaccine CV777 | Negative |
E1-4 | December 2019 | Inactivated vaccine CV777 | Negative |
E5 | December 2019 | Inactivated vaccine CV777 | Negative |
E6 | December 2019 | Inactivated vaccine CV777 | Positive |
1-1 | December 2019 | Inactivated vaccine CHYJ | Positive |
2-1 | December 2019 | Inactivated vaccine CHYJ | Negative |
3-1 | December 2019 | Inactivated vaccine CHYJ | Negative |
4-1 | December 2019 | Inactivated vaccine CHYJ | Negative |
5-1 | December 2019 | Inactivated vaccine CHYJ | Positive |
6-1 | December 2019 | Inactivated vaccine CHYJ | Negative |
1 + 2 | December 2019 | Duplex live attenuated vaccine LW/L | Positive |
JZ-p1 | December 2019 | Live attenuated triple vaccine CV777 | Negative |
JZ-p2 | December 2019 | Live attenuated triple vaccine CV777 | Negative |
7.1 | January 2020 | Inactivated vaccine CV777 | Positive |
7.2 | January 2020 | Inactivated vaccine CV777 | Positive |
7.3 | January 2020 | Inactivated vaccine CV777 | Negative |
ZMD3 | January 2020 | Live attenuated triple vaccine CV777 | Positive |
ZMD4 | January 2020 | Live attenuated triple vaccine CV777 | Positive |
ZMD5 | January 2020 | Live attenuated triple vaccine CV777 | Positive |
C1 | January 2020 | Duplex live attenuated vaccine SCSZ-1 | Positive |
C2 | January 2020 | Duplex live attenuated vaccine SCSZ-1 | Negative |
C3 | January 2020 | Duplex live attenuated vaccine SCSZ-1 | Negative |
C4 | January 2020 | Duplex live attenuated vaccine SCSZ-1 | Negative |
4-1 | November 2020 | Duplex live attenuated vaccine AJ1102 | Positive |
3-2 | November 2020 | Duplex live attenuated vaccine AJ1102 | Negative |
2-2 | November 2020 | Duplex live attenuated vaccine AJ1102 | Negative |
1-1 | November 2020 | Duplex live attenuated vaccine AJ1102 | Negative |
E7-8 | November 2020 | Inactivated vaccine CV777 | Negative |
E9 | November 2020 | Inactivated vaccine CV777 | Positive |
HN-01 | December 2020 | Inactivated vaccine CV777 | Negative |
HN-02 | December 2020 | Inactivated vaccine CV777 | Positive |
HN-03 | December 2020 | Inactivated vaccine CV777 | Positive |
HN-04 | December 2020 | Inactivated vaccine CV777 | Positive |
HN-05 | December 2020 | Inactivated vaccine CV777 | Negative |
J1 | December 2020 | Live attenuated triple vaccine CV777 | Negative |
J2 | December 2020 | Live attenuated triple vaccine CV777 | Negative |
J3 | December 2020 | Live attenuated triple vaccine CV777 | Positive |
SX1-3 | March 2021 | Duplex live attenuated vaccine AJ1102 | Negative |
SX4-5 | March 2021 | Duplex live attenuated vaccine AJ1102 | Positive |
SX6 | March 2021 | Duplex live attenuated vaccine AJ1102 | Positive |
SX7-9 | April 2021 | Duplex live attenuated vaccine AJ1102 | Negative |
SX10 | April 2021 | Duplex live attenuated vaccine AJ1102 | Positive |
Strain | GenBank Entry Number | Origin Country | Collection Year |
---|---|---|---|
CV777 | AF353511.1 | Belgium | 1978 |
LZC | EF185992.1 | China | 2006 |
DR13 | JQ023161.1 | Korea | 1999 |
AJ1102 | JX188454.1 | China | 2012 |
PC22A | KY499262.1 | USA | 2017 |
Missouri270/2014 | KR265846.1 | USA | 2016 |
S14 | MH891585.1 | Korea | 2019 |
MEX/JAL/03/2017 | MH004418.1 | Mexico | 2017 |
AH-2018-HF1 | MN315264.1 | China | 2018 |
G2 | MK138353.1 | China | 2018 |
CH-HB2-2018 | MK606369.1 | China | 2018 |
CH/HNLH/2015 | KT199103.1 | China | 2015 |
SM98 | GU937797.1 | Korea | 2011 |
JS2008 | KC109141.1 | China | 2013 |
YN90 | KT021231 | China | 2015 |
CH/ZMDZY/11 | KC196276 | China | 2013 |
NW8 | MF782687 | China | 2017 |
CH/YNKM-8/2013 | KF761675 | China | 2013 |
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Ge, Y.; Jiang, F.; Wang, S.; Wu, H.; Liu, Y.; Wang, B.; Hou, W.; Yu, X.; Wang, H. Natural Evolution of Porcine Epidemic Diarrhea Viruses Isolated from Maternally Immunized Piglets. Animals 2023, 13, 1766. https://doi.org/10.3390/ani13111766
Ge Y, Jiang F, Wang S, Wu H, Liu Y, Wang B, Hou W, Yu X, Wang H. Natural Evolution of Porcine Epidemic Diarrhea Viruses Isolated from Maternally Immunized Piglets. Animals. 2023; 13(11):1766. https://doi.org/10.3390/ani13111766
Chicago/Turabian StyleGe, Yufang, Feiyang Jiang, Sibei Wang, Heqiong Wu, Yuan Liu, Bin Wang, Wei Hou, Xiuju Yu, and Haidong Wang. 2023. "Natural Evolution of Porcine Epidemic Diarrhea Viruses Isolated from Maternally Immunized Piglets" Animals 13, no. 11: 1766. https://doi.org/10.3390/ani13111766
APA StyleGe, Y., Jiang, F., Wang, S., Wu, H., Liu, Y., Wang, B., Hou, W., Yu, X., & Wang, H. (2023). Natural Evolution of Porcine Epidemic Diarrhea Viruses Isolated from Maternally Immunized Piglets. Animals, 13(11), 1766. https://doi.org/10.3390/ani13111766