The Microbiota and Cytokines Correlation between the Jejunum and Colon in Altay Sheep
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals and Sample Collection
2.1.1. Animals
2.1.2. Intestinal Content Collection
2.1.3. Intestinal Tissue Collection
2.2. Microbiota Determination
2.2.1. Bacterial DNA Extraction
2.2.2. PCR Amplification
2.2.3. 16S Ribosomal RNA (rRNA) Gene Sequencing
2.3. Cytokine Determination
2.4. Statistical Analysis
3. Results
3.1. Intestinal Cytokine Diversities between the Jejunum and Colon
The Difference in the Concentration of Intestinal Cytokines
3.2. Microbiota Diversities
3.2.1. Sequencing Metrics
3.2.2. Taxonomic Characterization of Microbiota Composition
3.3. Correlations between Microbial Populations and Cytokines
4. Discussion
4.1. Cytokine Diversities between the Jejunum and Colon
4.2. Analysis of Microbiota Diversities in the Jejunum and Colon
4.3. Correlation of Intestinal Microbiota and Cytokines
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Diet Composition (%) | |
---|---|
Corn | 29.53 |
Cottonseed | 2.62 |
Soybean | 3.32 |
Bran | 6.56 |
CaHCO3 | 0.18 |
Limestone | 0.66 |
NaCl | 0.44 |
Premix | 0.44 |
Cornstalk | 56.25 |
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Ye, M.; Hou, M.; Peng, Q.; Jia, S.; Peng, B.; Yin, F.; Li, N.; Wang, J. The Microbiota and Cytokines Correlation between the Jejunum and Colon in Altay Sheep. Animals 2022, 12, 1564. https://doi.org/10.3390/ani12121564
Ye M, Hou M, Peng Q, Jia S, Peng B, Yin F, Li N, Wang J. The Microbiota and Cytokines Correlation between the Jejunum and Colon in Altay Sheep. Animals. 2022; 12(12):1564. https://doi.org/10.3390/ani12121564
Chicago/Turabian StyleYe, Mengjun, Meng Hou, Qimin Peng, Sheng Jia, Bin Peng, Fangfang Yin, Na Li, and Jinquan Wang. 2022. "The Microbiota and Cytokines Correlation between the Jejunum and Colon in Altay Sheep" Animals 12, no. 12: 1564. https://doi.org/10.3390/ani12121564