The Use of κ-Carrageenan in Egg Yolk Free Extender Improves the Efficiency of Canine Semen Cryopreservation
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Reagents and Composition of Extenders
2.2. Animals and Semen Collection
2.3. Experimental Design
2.4. Semen Cryopreservation and Thawing
2.5. Assessment of Frozen-Thawed Semen Motility
2.6. Assessment of Plasma Membrane Integrity
2.7. Assessment of Acrosome Integrity
2.8. Apoptosis Index
2.9. ROS Level
2.10. Gene Expression Analysis
2.11. Statistical Analysis
3. Results
3.1. Effect of κ-carrageenan on Sperm Motility
3.2. Effect of κ-carrageenan on Sperm Plasma Membrane Integrity
3.3. Effect of κ-carrageenan on Acrosome Integrity
3.4. Effect of κ-carrageenan on Apoptosis
3.5. Effect of κ-carrageenan on ROS Levels
3.6. Effect of κ-carrageenan on Gene Expression
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Primer Sequences | Product Size (bp) | Accession Number |
---|---|---|---|
GAPDH | F: GGA GAA AGC TGC CAA ATA TG R: ACC AGG AAA TGA GCT TGA CA | 151 | NM_001003142.2 |
Bcl-2 | F: CTC CTG GCT GTC TCT GAA GG R: GTG GCA GGC CTA CTG ACT TC | 145 | NM_001002949.1 |
BAX | F: GAC GGC CTC CTC TCC TAC TT R: GGT GAG TGA CGC AGT AAG CA | 120 | NM_001003011.1 |
NOX5 | F: ACC TGA ACA TCC CCA CCA TC R: TTC AGA CCG GAT GTG TAG CC | 101 | NM_001103218.1 |
SMOX | F: AGA AGT GTG ATG ACG AGG CG R: TCG GAA GTA TGG GTT GCT GC | 128 | XM_855324.3 |
SMS | F: GTC GCC TGG TTG AGT ATG ACA R: ATG CCA AAT CAC TCT CCG CC | 144 | XM_005641195.1 |
Groups | TM (%) | RPM (%) | MPM (%) | IM (%) |
---|---|---|---|---|
Control | 40.2 ± 8.3 | 30.3 ± 4.1 | 9.9 ± 4.1 | 59.8 ± 8.3 |
0.1% κ-carrageenan | 63.3 ± 6.8 | 57.6 ± 6.3 * | 5.6 ± 0.4 | 36.7 ± 6.8 |
0.2% κ-carrageenan | 64.3 ± 0.5 * | 56.5 ± 1.4 * | 7.8 ± 1.8 | 35.7 ± 0.5 * |
0.3% κ-carrageenan | 20.4 ± 0.2 | 17.7 ± 0.3 | 2.6 ± 0.5 | 79.6 ± 0.2 |
0.5% κ-carrageenan | 28.3 ± 9.3 | 25.3 ± 9.5 | 3.0 ± 0.2 | 71.7 ± 9.3 |
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Kim, E.; Almubarak, A.; Talha, N.; Yu, I.-J.; Jeon, Y. The Use of κ-Carrageenan in Egg Yolk Free Extender Improves the Efficiency of Canine Semen Cryopreservation. Animals 2022, 12, 88. https://doi.org/10.3390/ani12010088
Kim E, Almubarak A, Talha N, Yu I-J, Jeon Y. The Use of κ-Carrageenan in Egg Yolk Free Extender Improves the Efficiency of Canine Semen Cryopreservation. Animals. 2022; 12(1):88. https://doi.org/10.3390/ani12010088
Chicago/Turabian StyleKim, Eunji, Areeg Almubarak, Nabeel Talha, Il-Jeoung Yu, and Yubyeol Jeon. 2022. "The Use of κ-Carrageenan in Egg Yolk Free Extender Improves the Efficiency of Canine Semen Cryopreservation" Animals 12, no. 1: 88. https://doi.org/10.3390/ani12010088
APA StyleKim, E., Almubarak, A., Talha, N., Yu, I.-J., & Jeon, Y. (2022). The Use of κ-Carrageenan in Egg Yolk Free Extender Improves the Efficiency of Canine Semen Cryopreservation. Animals, 12(1), 88. https://doi.org/10.3390/ani12010088