Associations among Farm, Breed, Lactation Stage and Parity, Gene Polymorphisms and the Fatty Acid Profile of Milk from Holstein, Simmental and Their Crosses
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Fatty Acids Analyses
2.3. Genotyping
2.4. Statistical Analysis
3. Results and Discussion
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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AGPAT6 | DGAT1 | LEP | FASN | SCD1 | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
CC | CT | TT | AA | AK | MM | MW | WW | AG | GG | CC | CT | TT |
256 | 560 | 14 | 816 | 44 | 542 | 130 | 26 | 206 | 652 | 226 | 546 | 84 |
FAs and FA Groups 2 | AGPAT6 | DGAT1 | LEP | FASN | SCD1 | |
---|---|---|---|---|---|---|
C10:0 | 4 | 0.010 TT A > CT a > CC Bb | ||||
C10:1 * | 5 | <0.0001 CC A > CT B > TT C | ||||
C12:0 | 7 | 0.028 TT a > CT a > CC b | ||||
C12:1 * | 8 | <0.0001 CC Aa > CT Ab > TT B | ||||
isoC14:0 | 10 | 0.045 * AG a > GG b | ||||
C14:0 | 11 | 0.050 AG > GG | 0.007 TT Ab > CT a > CC Bb | |||
C14:1n-5c | 12 | <0.0001 CC A > CT B > TT C | ||||
C15:0 | 14 | 0.062 WW > MM > MW | ||||
isoC16:0 | 16 | 0.074 AG > GG | ||||
C16:1n-7c | 20 | 0.002 TT a > CT A > CC Bb | ||||
isoC17:0 | 21 | 0.013 CC A > CT A > TT B | ||||
C16:1 * | 22 | 0.042 AG a > GG b | 0.026 CC A > CT a > TT Bb | |||
anteisoC17:0 | 23 | 0.011 WW Aa > MW b > MM B | ||||
C18:1n-7t | 25 | 0.088 TT > CT > CC | 0.075 AG > GG | |||
C18:1 * | 32 | 0.082 AG > GG | 0.018 CC a > TT b > CT b | |||
C18:2 * | 33 | 0.0006 TT A > CT A > CC B | ||||
C19:1 * | 35 | 0.063 WW > MW, MM | ||||
C18:3 * | 36 | 0.0002 WW A > MW B, MM B | ||||
C20:1n-9c | 40 | 0.046 AG a > GG b | ||||
C20:4n-6 ** | 44 | 0.095 TT > CT > CC | 0.006 WW Aa > MW b > MM B | |||
C22:0 | 45 | 0.030 AA a > AK b | ||||
C20:4n-3 ** | 46 | 0.003 AK A > AA B | 0.066 AG > GG | |||
C20:5n-3 ** | 47 | 0.042 WW a > MM b, MW b | ||||
C24:0 | 48 | 0.096 CT > TT > CC | ||||
C22:5n-3 ** | 49 | 0.005 WW A > MM B, MW B | 0.021 AG a > GG b | |||
PUFAn-3 | 54 | 0.059 WW > MM > MW | ||||
BCFA | 59 | 0.029 WW a > MW > MM b | 0.048 AG a > GG b |
FAs and FA Groups 1 | Effects | Interaction | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Farm | Gene | Lactation Parity | Lactation Stage | Breed of the Cow | Genotype * Farm | Genotype * Lactation Stage | Farm * Lactation Stage | Lactation Parity * Lactation Stage | ||
C4:0 | 1 | *** | ns | ns | *** | ns | ns | ns | ** | ns |
C6:0 | 2 | *** | ns | ns | ** | ns | N/A | N/A | * | ns |
C8:0 | 3 | *** | ns | ns | ns | ns | ns | ns | ** | ns |
C10:0 | 4 | *** | ns | ns | ns | ns | N/A | N/A | ** | ns |
C10:1 * | 5 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
C11:0 | 6 | *** | ns | ns | ** | ns | ns | ns | * | ns |
C12:0 | 7 | ** | ns | ns | ** | ns | ns | ns | ** | ns |
C12:1 * | 8 | * | ns | * | *** | ns | N/A | ns | ns | ns |
C13:0 | 9 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
isoC14:0 | 10 | * | ns | * | ** | * | ns | ns | ns | ns |
C14:0 | 11 | *** | ns | ns | *** | ns | N/A | ns | ns | ns |
C14:1n-5c | 12 | *** | ns | * | *** | ** | N/A | ns | ns | ns |
anteisoC15:0 | 13 | *** | ns | ns | ** | ns | ns | ns | ns | ns |
C15:0 | 14 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
C15:1 * | 15 | *** | ns | ns | ns | ns | N/A | ns | *** | * |
isoC16:0 | 16 | *** | ns | ns | * | * | ns | ns | ns | ns |
C16:0 | 17 | *** | ns | ns | *** | ns | ns | ns | ** | ns |
C16:1 * | 18 | *** | ns | ns | *** | ns | ns | ns | *** | ns |
C16:1 * | 19 | *** | ns | ns | *** | ns | N/A | ns | ns | ns |
C16:1n-7c | 20 | ns | ns | * | * | * | N/A | ns | ** | ns |
isoC17:0 | 21 | *** | ns | ns | ** | ns | ns | ns | ns | ns |
C16:1 * | 22 | ** | ns | *** | *** | ns | ns | ns | ns | ns |
anteisoC17:0 | 23 | *** | ns | ns | ns | ns | ns | ns | ns | ns |
C17:0 | 24 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
C17:1n-7c | 25 | *** | ns | ns | *** | ns | N/A | ns | *** | ns |
C18:0 | 26 | *** | ns | * | *** | ns | N/A | ns | ** | ns |
C18:1t * | 27 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
C18:1n-7t | 28 | *** | ns | ns | *** | ns | ns | ns | ** | ns |
C18:1n-9c | 29 | *** | ns | ns | *** | ns | ns | ns | ** | ns |
C18:1n-7c | 30 | *** | ns | ns | *** | ns | N/A | ns | ns | ns |
C18:1 * | 31 | *** | ns | ns | *** | ns | N/A | ns | ** | ns |
C18:1 * | 32 | *** | ns | ns | *** | ns | N/A | ns | ns | ns |
C18:2 * | 33 | *** | ns | ns | *** | ns | N/A | ns | ns | ns |
C18:2n-6 ** | 34 | *** | ns | ns | ns | ns | ns | ns | ** | ns |
C19:1 * | 35 | *** | ns | ns | ** | ns | N/A | ns | *** | ns |
C18:3 * | 36 | *** | ns | ns | ns | ns | N/A | N/A | ns | ** |
C18:3n-3 ** | 37 | *** | ns | ns | * | ns | N/A | ns | ns | ns |
C20:0 | 38 | *** | ns | ** | *** | * | N/A | ns | ns | ns |
C18:2c9,t11 | 39 | *** | ns | ns | *** | ns | ns | ns | * | ns |
C20:1n-9c | 40 | *** | ns | ns | *** | ns | ns | ns | * | ns |
C20:1n-7c | 41 | *** | ns | ns | *** | ns | ns | ns | ** | ns |
C21:0 | 42 | *** | ns | ns | ns | ns | ns | ns | ns | ns |
C20:3n-6 ** | 43 | *** | ns | ns | ** | ns | N/A | ns | ns | ns |
C20:4n-6 ** | 44 | *** | ns | ns | ns | ns | N/A | ns | *** | ns |
C22:0 | 45 | *** | ns | ns | ** | ** | ns | ns | * | ns |
C20:4n-3 ** | 46 | *** | ns | ns | ** | * | * | ns | ns | ns |
C20:5n-3 ** | 47 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
C24:0 | 48 | *** | ns | ns | * | ns | ns | ns | ns | *** |
C22:5n-3 ** | 49 | *** | ns | *** | *** | ns | ns | ns | ns | ns |
SFA | 50 | *** | ns | ns | * | ns | ns | ns | * | ns |
MUFA | 51 | *** | ns | ns | ** | ns | ns | ns | ** | ns |
TFA | 52 | *** | ns | ns | *** | ns | N/A | ns | * | ns |
PUFA | 53 | *** | ns | ns | ns | ns | N/A | ns | ns | ns |
PUFAn-3 | 54 | *** | ns | * | *** | ns | ns | ns | ns | ns |
SCFA | 55 | *** | ns | ns | ns | ns | ns | ns | ** | ns |
MCFA | 56 | *** | ns | ns | *** | ns | N/A | ns | ** | ns |
LCFA | 57 | *** | ns | ns | *** | ns | ns | ns | * | ns |
UFA | 58 | *** | ns | ns | * | ns | N/A | N/A | * | ns |
BCFA | 59 | *** | ns | * | ** | ns | ns | ns | ns | ns |
C18 | 60 | *** | ns | * | *** | ns | ns | N/A | * | * |
FAs and FA Groups 1 | Effects | Interaction | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Farm | Gene | Lactation Parity | Lactation Stage | Breed of the Cow | Genotype * Farm | Genotype * Lactation Stage | Farm * Lactation Stage | Lactation Parity * Lactation Stage | ||
C4:0 | 1 | *** | ns | *** | ns | ns | * | ** | ** | ns |
C6:0 | 2 | *** | ns | * | ns | ns | ** | * | * | ns |
C8:0 | 3 | *** | ns | ns | ns | ns | * | ** | ** | ns |
C10:0 | 4 | ** | ns | ns | ns | ns | * | ** | ** | ns |
C10:1 * | 5 | *** | * | *** | ns | ns | * | ns | ns | ns |
C11:0 | 6 | *** | ns | ** | ns | ns | ns | * | * | ns |
C12:0 | 7 | ** | * | ** | ns | ns | * | ** | ** | ns |
C12:1 * | 8 | * | * | *** | ns | ns | ns | ns | ns | ns |
C13:0 | 9 | *** | ns | *** | ns | ns | ns | ns | ns | ns |
isoC14:0 | 10 | * | ns | * | *** | * | ns | ns | ns | ns |
C14:0 | 11 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
C14:1n-5c | 12 | *** | ns | * | *** | ** | ns | ns | ns | ns |
anteisoC15:0 | 13 | *** | ns | ns | ** | ns | ns | ns | ns | ns |
C15:0 | 14 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
C15:1 * | 15 | *** | ns | ns | ns | ns | ns | ns | *** | * |
isoC16:0 | 16 | *** | ns | ns | * | * | ns | ns | ns | ns |
C16:0 | 17 | *** | ns | ns | *** | * | ns | ns | ** | ns |
C16:1 * | 18 | *** | ns | ns | *** | ns | ns | ns | *** | * |
C16:1* | 19 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
C16:1n-7c | 20 | ns | ns | * | * | * | ns | ns | * | ns |
isoC17:0 | 21 | *** | ns | ns | ** | ns | ns | ns | ns | ns |
C16:1 * | 22 | ** | ns | *** | *** | ns | ns | ns | ns | ns |
anteisoC17:0 | 23 | *** | ns | ns | ns | ns | ns | ns | * | ns |
C17:0 | 24 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
C17:1n-7c | 25 | *** | ns | ns | *** | ns | ns | ** | *** | ns |
C18:0 | 26 | *** | ns | * | *** | ns | ns | ns | * | ns |
C18:1t * | 27 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
C18:1n-7t | 28 | *** | ns | ns | *** | ns | ns | ns | *** | ns |
C18:1n-9c | 29 | *** | ns | ns | *** | ns | ns | ns | ** | ns |
C18:1n-7c | 30 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
C18:1 * | 31 | *** | ns | ns | *** | ns | ns | ns | *** | ns |
C18:1 * | 32 | *** | ns | ns | ** | ns | ns | ns | ns | ns |
C18:2 * | 33 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
C18:2n-6 ** | 34 | *** | ns | ns | ns | ns | ns | ns | ** | ns |
C19:1 * | 35 | *** | ns | ns | ** | ns | ns | * | *** | ns |
C18:3 * | 36 | *** | ns | ns | ns | ns | *** | ns | ns | ** |
C18:3n-3 ** | 37 | *** | ns | ns | * | ns | ns | ns | ns | ns |
C20:0 | 38 | *** | ns | ** | *** | ns | ns | ns | ns | ns |
C18:2c9,t11 | 39 | *** | ns | ns | *** | ns | ns | ns | * | ns |
C20:1n-9c | 40 | *** | ns | ns | *** | ns | ns | ns | ** | ns |
C20:1n-7c | 41 | *** | ns | ns | *** | ns | * | ns | ** | ns |
C21:0 | 42 | *** | ns | ns | ns | ns | ns | ns | ns | ns |
C20:3n-6 ** | 43 | ** | ns | ns | ** | ns | ** | ns | ns | ns |
C20:4n-6 ** | 44 | ** | ns | ns | ns | ns | ns | ns | *** | ns |
C22:0 | 45 | *** | * | ns | ** | * | ns | ns | * | ns |
C20:4n-3 ** | 46 | *** | ** | ns | ** | * | ns | ** | ns | ns |
C20:5n-3 ** | 47 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
C24:0 | 48 | *** | ns | ns | * | ns | ns | ns | ns | ** |
C22:5n-3 ** | 49 | *** | ns | *** | *** | ns | ns | ns | * | ns |
SFA | 50 | *** | ns | ns | ** | ns | ns | ns | * | ns |
MUFA | 51 | *** | ns | ns | ** | ns | ns | ns | ** | ns |
TFA | 52 | *** | ns | ns | *** | ns | ns | ns | * | ns |
PUFA | 53 | *** | ns | ns | ns | ns | ns | ns | ns | ns |
PUFAn-3 | 54 | *** | ns | ** | *** | ns | ns | ns | ns | * |
SCFA | 55 | *** | ns | ns | ns | ns | ns | ** | ** | ns |
MCFA | 56 | *** | ns | ns | *** | ns | ns | ns | ** | ns |
LCFA | 57 | *** | ns | * | *** | ns | ns | ns | * | ns |
UFA | 58 | *** | ns | ns | ** | ns | ns | ns | * | ns |
BCFA | 59 | *** | ns | * | *** | ns | ns | ns | ns | * |
C18 | 60 | *** | ns | * | *** | ns | ns | ns | ** | ns |
FAs and FA Groups 1 | Effects | Interaction | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Farm | Gene | Lactation Parity | Lactation Stage | Breed of the Cow | Genotype * Farm | Genotype * Lactation Stage | Farm * Lactation Stage | Lactation Parity * Lactation Stage | ||
C4:0 | 1 | *** | ns | ns | *** | ns | ns | ns | ** | ns |
C6:0 | 2 | *** | ns | ns | * | ns | ns | ns | * | ns |
C8:0 | 3 | *** | ns | ns | ns | ns | ns | ns | ** | ns |
C10:0 | 4 | ** | ns | ns | ns | ns | ns | ns | ** | ns |
C10:1 * | 5 | *** | ns | * | *** | ns | ns | ns | ** | ns |
C11:0 | 6 | *** | ns | ns | ** | ns | ns | ns | * | ns |
C12:0 | 7 | *** | ns | * | ** | ns | ns | ns | ** | ns |
C12:1 * | 8 | ns | ns | * | *** | ns | ns | ns | * | ns |
C13:0 | 9 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
isoC14:0 | 10 | ns | * | ns | ** | * | ns | ns | ns | ns |
C14:0 | 11 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
C14:1n-5c | 12 | *** | ns | * | *** | * | ns | ns | * | ns |
anteisoC15:0 | 13 | *** | ns | ns | ** | ns | ns | ns | ns | ns |
C15:0 | 14 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
C15:1 * | 15 | *** | ns | ns | ns | ns | ns | ns | *** | * |
isoC16:0 | 16 | *** | ns | ns | * | * | ns | ns | ns | ns |
C16:0 | 17 | *** | ns | ns | *** | * | ns | ns | ** | ns |
C16:1 * | 18 | *** | ns | ns | *** | ns | ns | ns | *** | * |
C16:1 * | 19 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
C16:1n-7c | 20 | ns | ns | * | ns | * | ns | ns | ** | ns |
isoC17:0 | 21 | *** | ns | ns | ** | ns | ns | ns | ns | ns |
C16:1 * | 22 | *** | * | *** | *** | ns | ns | ns | * | ns |
anteisoC17:0 | 23 | *** | ns | ns | ns | ns | ns | ns | ns | ns |
C17:0 | 24 | *** | ns | * | *** | ns | ns | ns | ns | ns |
C17:1n-7c | 25 | *** | ns | ns | *** | * | * | ns | *** | ns |
C18:0 | 26 | *** | ns | ** | *** | ns | ns | ns | *** | ns |
C18:1t * | 27 | *** | ns | ns | *** | ns | ns | ns | * | ns |
C18:1n-7t | 28 | *** | ns | ns | *** | ns | ns | ns | *** | ns |
C18:1n-9c | 29 | *** | ns | ns | *** | ns | ns | ns | ** | ns |
C18:1n-7c | 30 | *** | ns | ns | *** | ns | ns | ns | ** | ns |
C18:1 * | 31 | *** | ns | ns | *** | ns | ns | ns | *** | ns |
C18:1 * | 32 | *** | ns | ns | ** | ns | ns | ns | * | ns |
C18:2 * | 33 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
C18:2n-6 ** | 34 | *** | ns | ns | ns | ns | * | ns | ** | ns |
C19:1 * | 35 | *** | ns | ns | ** | ns | ** | ns | *** | ns |
C18:3 * | 36 | *** | ns | ns | ns | ns | ns | ns | ns | ** |
C18:3n-3 ** | 37 | *** | ns | ns | * | ns | ns | ns | ns | ns |
C20:0 | 38 | *** | ns | ** | *** | * | * | ns | * | ns |
C18:2c9,t11 | 39 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
C20:1n-9c | 40 | *** | * | ns | *** | ns | ns | ns | * | ns |
C20:1n-7c | 41 | *** | ns | * | *** | ns | ns | ns | ** | ns |
C21:0 | 42 | *** | ns | ns | ns | ns | ns | ns | ns | ns |
C20:3n-6 ** | 43 | ** | ns | ns | ** | ns | ns | ns | ns | * |
C20:4n-6 ** | 44 | ** | ns | ns | ns | ns | ns | ns | *** | ns |
C22:0 | 45 | *** | ns | ns | ** | * | ns | ns | * | ns |
C20:4n-3 ** | 46 | *** | ns | ns | ** | ** | ns | ns | ns | * |
C20:5n-3 ** | 47 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
C24:0 | 48 | *** | ns | ns | * | ns | ns | ns | ns | ** |
C22:5n-3 ** | 49 | *** | * | *** | *** | ns | ns | ns | ns | ns |
SFA | 50 | *** | ns | ns | ** | ns | ns | ns | * | ns |
MUFA | 51 | *** | ns | ns | ** | ns | ns | ns | ** | ns |
TFA | 52 | *** | ns | ns | *** | ns | ns | ns | * | ns |
PUFA | 53 | *** | ns | ns | ns | ns | ns | ns | ns | ns |
PUFAn-3 | 54 | *** | ns | ** | *** | ns | ns | ns | ns | ** |
SCFA | 55 | *** | ns | ns | ns | ns | ns | ns | ** | ns |
MCFA | 56 | *** | ns | * | *** | ns | ns | ns | *** | ns |
LCFA | 57 | *** | ns | * | *** | ns | ns | ns | ** | ns |
UFA | 58 | *** | ns | ns | ** | ns | ns | ns | * | ns |
BCFA | 59 | *** | * | * | ** | ns | ns | ns | ns | * |
C18 | 60 | *** | ns | * | *** | ns | ns | ns | ** | ns |
FAs and FA Groups 1 | Effects | Interaction | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Farm | Gene | Lactation Parity | Lactation Stage | Breed of the Cow | Genotype * Farm | Genotype * Lactation Stage | Farm * Lactation Stage | Lactation Parity * Lactation Stage | ||
C4:0 | 1 | *** | ns | ns | *** | ns | ns | ns | *** | ns |
C6:0 | 2 | *** | ns | ns | * | ns | ns | ns | * | ns |
C8:0 | 3 | *** | ns | ns | ns | ns | ns | * | ** | ns |
C10:0 | 4 | *** | ns | ns | ns | ns | ns | ns | *** | ns |
C10:1 * | 5 | *** | ns | ** | *** | ns | ns | ns | *** | ns |
C11:0 | 6 | *** | ns | ns | * | ns | ns | * | ** | ns |
C12:0 | 7 | *** | ns | ns | ** | ns | ns | ns | *** | ns |
C12:1 * | 8 | ** | ns | * | *** | ns | ns | * | ** | ns |
C13:0 | 9 | *** | ns | ns | *** | ns | ns | ** | * | ns |
isoC14:0 | 10 | ns | ns | ns | *** | * | ns | ns | ns | ns |
C14:0 | 11 | *** | ns | ns | *** | ns | ns | ns | * | ns |
C14:1n-5c | 12 | *** | ns | * | *** | ** | ns | ns | * | ns |
anteisoC15:0 | 13 | *** | ns | ns | ** | ns | ns | ns | ns | ns |
C15:0 | 14 | *** | ns | ns | *** | ns | ns | * | ns | ns |
C15:1 * | 15 | *** | ns | ns | ns | ns | ns | ns | *** | * |
isoC16:0 | 16 | ** | ns | ns | * | * | ns | ns | ns | ns |
C16:0 | 17 | *** | ns | ns | *** | ns | ns | ns | ** | ns |
C16:1* | 18 | *** | ns | ns | *** | ns | ns | ns | *** | ns |
C16:1 * | 19 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
C16:1n-7c | 20 | ns | ns | ns | ns | * | ns | ns | * | ns |
isoC17:0 | 21 | *** | ns | ns | ** | ns | ns | ns | ns | ns |
C16:1* | 22 | *** | ns | *** | *** | ns | ns | ns | ** | ns |
anteisoC17:0 | 23 | *** | * | ns | ns | ns | ns | ns | ns | ns |
C17:0 | 24 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
C17:1n-7c | 25 | *** | ns | ns | *** | ns | ns | ns | *** | ns |
C18:0 | 26 | *** | ns | ** | *** | ns | ns | ns | *** | ns |
C18:1t * | 27 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
C18:1n-7t | 28 | *** | ns | ns | *** | ns | ns | ns | ** | ns |
C18:1n-9c | 29 | *** | ns | ns | *** | ns | ns | ns | *** | ns |
C18:1n-7c | 30 | *** | ns | * | *** | ns | ns | ns | ns | ns |
C18:1 * | 31 | *** | ns | ns | *** | ns | ns | ns | * | ns |
C18:1 * | 32 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
C18:2 * | 33 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
C18:2n-6 ** | 34 | *** | ns | ns | ns | ns | ns | ns | ** | * |
C19:1 * | 35 | *** | ns | ns | * | ns | ns | ns | *** | ns |
C18:3 * | 36 | *** | *** | ns | ns | ns | *** | ns | ns | ** |
C18:3n-3 ** | 37 | *** | ns | ns | * | ns | ns | ns | ns | ns |
C20:0 | 38 | *** | ns | ** | ** | * | ns | ns | * | ns |
C18:2c9,t11 | 39 | *** | ns | ns | *** | ns | ns | ns | * | ns |
C20:1n-9c | 40 | *** | ns | ns | *** | ns | ns | ns | * | ns |
C20:1n-7c | 41 | *** | ns | * | *** | ns | * | ns | * | ns |
C21:0 | 42 | *** | ns | ns | ns | ns | ns | ns | ns | ns |
C20:3n-6 ** | 43 | * | ns | ns | ** | ns | ** | ns | ns | ns |
C20:4n-6 ** | 44 | * | ** | ns | ns | ns | ns | ns | *** | ns |
C22:0 | 45 | *** | ns | ns | * | * | ns | ns | * | ns |
C20:4n-3 ** | 46 | *** | ns | ns | * | * | ns | ns | ns | * |
C20:5n-3 ** | 47 | *** | * | ns | *** | ns | ns | ns | ns | ns |
C24:0 | 48 | *** | ns | ns | * | ns | ns | ns | ns | ns |
C22:5n-3 ** | 49 | *** | ** | *** | *** | ns | ns | ns | * | * |
SFA | 50 | *** | ns | ns | * | ns | ns | ns | * | * |
MUFA | 51 | *** | ns | ns | * | ns | ns | ns | *** | *** |
TFA | 52 | *** | ns | ns | *** | ns | ns | ns | * | * |
PUFA | 53 | *** | ns | ns | ns | ns | ns | ns | ns | ns |
PUFAn-3 | 54 | *** | ns | * | *** | ns | ns | ns | ns | * |
SCFA | 55 | *** | ns | ns | ns | ns | ns | * | ** | ns |
MCFA | 56 | *** | ns | ns | *** | ns | ns | ns | *** | ns |
LCFA | 57 | *** | ns | * | *** | ns | ns | ns | *** | ns |
UFA | 58 | *** | ns | ns | * | ns | ns | ns | ** | ns |
BCFA | 59 | *** | * | * | *** | ns | ns | ns | * | * |
C18 | 60 | *** | ns | * | *** | ns | ns | ns | *** | ns |
FAs and FA Groups 1 | Effects | Interaction | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Farm | Gene | Lactation Parity | Lactation Stage | Breed of the Cow | Genotype * Farm | Genotype * Lactation Stage | Farm * Lactation Stage | Lactation Parity * Lactation Stage | ||
C4:0 | 1 | *** | ns | ns | *** | ns | ns | ns | ** | ns |
C6:0 | 2 | *** | ns | ns | * | ns | ns | ns | * | ns |
C8:0 | 3 | *** | ns | ns | ns | ns | ns | ns | ** | ns |
C10:0 | 4 | ** | * | ns | ns | ns | ns | ns | ** | ns |
C10:1 * | 5 | *** | *** | * | *** | ns | ns | ns | ** | ns |
C11:0 | 6 | *** | ns | ns | ** | ns | ns | ns | * | ns |
C12:0 | 7 | ** | * | * | ** | ns | ns | ns | ** | ns |
C12:1 * | 8 | * | *** | * | *** | ns | ns | ns | * | ns |
C13:0 | 9 | *** | ns | ns | *** | ns | ns | ns | ns | ns |
isoC14:0 | 10 | ns | ns | * | *** | * | ns | ns | ns | ns |
C14:0 | 11 | ns | ** | ns | *** | ns | ns | ns | ns | ns |
C14:1n-5c | 12 | ns | *** | * | *** | * | ns | ns | ns | ns |
anteisoC15:0 | 13 | ns | ns | ns | ** | ns | ns | ns | ns | ns |
C15:0 | 14 | ns | ns | ns | *** | ns | ns | ns | ns | ns |
C15:1 * | 15 | ns | ns | ns | ns | ns | ns | ns | *** | * |
isoC16:0 | 16 | ns | ns | ns | * | * | ns | ns | ns | ns |
C16:0 | 17 | ns | ns | ns | *** | * | ns | ns | ** | ns |
C16:1 * | 18 | ns | ns | ns | *** | ns | ns | ns | *** | * |
C16:1 * | 19 | ns | ns | ns | *** | ns | ns | * | ns | ns |
C16:1n-7c | 20 | ns | ** | * | ns | * | ns | ns | *** | ns |
isoC17:0 | 21 | *** | * | ns | ** | ns | ns | ns | ns | ns |
C16:1 * | 22 | ** | * | *** | *** | ns | ns | ns | ns | ns |
anteisoC17:0 | 23 | *** | ns | ns | ns | ns | ns | ns | ns | * |
C17:0 | 24 | *** | ns | * | *** | ns | ns | ns | ns | ns |
C17:1n-7c | 25 | *** | ns | ns | *** | ns | ns | ns | *** | ns |
C18:0 | 26 | *** | ns | ** | *** | ns | ns | ns | *** | ns |
C18:1t * | 27 | *** | ns | ns | *** | ns | ns | ns | * | ns |
C18:1n-7t | 28 | *** | ns | ns | *** | ns | ns | ns | *** | ns |
C18:1n-9c | 29 | *** | ns | ns | *** | ns | ns | ns | ** | ns |
C18:1n-7c | 30 | *** | ns | * | *** | ns | ns | ns | ns | ns |
C18:1 * | 31 | *** | ns | ns | *** | ns | ns | ns | *** | ns |
C18:1 * | 32 | *** | * | ns | ** | ns | ns | ns | * | ns |
C18:2 * | 33 | *** | *** | ns | *** | ns | ns | ns | ns | ns |
C18:2n-6 ** | 34 | *** | ns | ns | ns | ns | ns | ns | ** | ns |
C19:1 * | 35 | *** | ns | ns | * | ns | ns | ns | *** | * |
C18:3 * | 36 | *** | ns | ns | ns | ns | ns | * | ns | ** |
C18:3n-3 ** | 37 | *** | ns | ns | * | ns | ns | ns | ns | ns |
C20:0 | 38 | *** | ns | ** | *** | * | ns | ns | * | ns |
C18:2c9,t11 | 39 | *** | ns | ns | *** | ns | ns | ns | * | ns |
C20:1n-9c | 40 | *** | ns | ns | *** | ns | ns | ns | * | ns |
C20:1n-7c | 41 | *** | ns | ns | *** | ns | ns | ns | ** | ns |
C21:0 | 42 | *** | ns | ns | ns | ns | ns | ns | ns | ns |
C20:3n-6 ** | 43 | ** | ns | ns | ** | ns | ** | ns | ns | ns |
C20:4n-6 ** | 44 | ** | ns | ns | ns | ns | ns | ns | *** | ns |
C22:0 | 45 | *** | ns | ns | * | * | ns | ns | * | ns |
C20:4n-3 ** | 46 | *** | ns | ns | ** | * | ns | ns | ns | * |
C20:5n-3 ** | 47 | *** | ns | ns | *** | ns | * | ns | ns | ns |
C24:0 | 48 | *** | ns | ns | * | ns | ns | ns | ns | ** |
C22:5n-3 ** | 49 | *** | ns | *** | *** | ns | ns | ns | ns | ns |
SFA | 50 | *** | ns | ns | ** | ns | ns | ns | * | ns |
MUFA | 51 | *** | ns | ns | ** | ns | ns | ns | ** | ns |
TFA | 52 | *** | ns | ns | *** | ns | ns | ns | ** | ns |
PUFA | 53 | *** | ns | ns | ns | ns | ns | ns | ns | ns |
PUFAn-3 | 54 | *** | ns | ** | *** | ns | ns | ns | ns | ** |
SCFA | 55 | *** | ns | ns | ns | ns | ns | ns | ** | ns |
MCFA | 56 | *** | ns | ns | *** | ns | ns | ns | ** | ns |
LCFA | 57 | *** | ns | * | *** | ns | ns | ns | ** | ns |
UFA | 58 | *** | ns | ns | ** | ns | ns | ns | * | ns |
BCFA | 59 | *** | ns | * | ** | ns | ns | ns | ns | * |
C18 | 60 | *** | ns | * | *** | ns | ns | ns | ** | ns |
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Samková, E.; Čítek, J.; Brzáková, M.; Hanuš, O.; Večerek, L.; Jozová, E.; Hoštičková, I.; Trávníček, J.; Hasoňová, L.; Rost, M.; et al. Associations among Farm, Breed, Lactation Stage and Parity, Gene Polymorphisms and the Fatty Acid Profile of Milk from Holstein, Simmental and Their Crosses. Animals 2021, 11, 3284. https://doi.org/10.3390/ani11113284
Samková E, Čítek J, Brzáková M, Hanuš O, Večerek L, Jozová E, Hoštičková I, Trávníček J, Hasoňová L, Rost M, et al. Associations among Farm, Breed, Lactation Stage and Parity, Gene Polymorphisms and the Fatty Acid Profile of Milk from Holstein, Simmental and Their Crosses. Animals. 2021; 11(11):3284. https://doi.org/10.3390/ani11113284
Chicago/Turabian StyleSamková, Eva, Jindřich Čítek, Michaela Brzáková, Oto Hanuš, Libor Večerek, Eva Jozová, Irena Hoštičková, Jan Trávníček, Lucie Hasoňová, Michael Rost, and et al. 2021. "Associations among Farm, Breed, Lactation Stage and Parity, Gene Polymorphisms and the Fatty Acid Profile of Milk from Holstein, Simmental and Their Crosses" Animals 11, no. 11: 3284. https://doi.org/10.3390/ani11113284