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Using Time Lapse Monitoring for Determination of Morphological Defect Frequency in Feline Embryos after in Vitro Fertilization (IVF)

1
Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture, Mickiewicza 24/28, 30-059 Krakow, Poland
2
Department of Reproduction and Clinic of Farm Animals, University of Environmental Science, Grundwaldzki square 49, 50-357 Wroclaw, Poland
3
Department of Animal Nutrition, Biotechnology and Fishering, University of Agriculture, Mickiewicza 24/28, 30-059 Krakow, Poland
*
Author to whom correspondence should be addressed.
Animals 2020, 10(1), 3; https://doi.org/10.3390/ani10010003
Received: 18 November 2019 / Revised: 13 December 2019 / Accepted: 14 December 2019 / Published: 18 December 2019
(This article belongs to the Section Animal Reproduction)
This study was conducted with the aim of determining the frequency of morphological defects in feline embryos, their competence to reach the blastocyst stage, and their ability to hatch. Embryonic morphological disorders affect development potential, and the use of time lapse monitoring (TLM) guarantees the precise observation of any changes that occur during in vitro embryo development.
Some human, bovine, and mouse in vitro fertilized (IVF) embryos with morphokinetic abnormalities such as fragmentation, direct cleavage, and cytoplasmic vacuoles have the potential to reach the blastocyst stage, which is related to a high potential for implantation. The latest techniques of embryo development observation to enable the evaluation and selection of embryos are based on time lapse monitoring (TLM). The aim of this study was to determine the frequency of morphological defects in feline embryos, their competence to reach the blastocyst stage, and their ability to hatch. Oocyte-cumulus complexes were isolated after the scarification of ovaries and matured in vitro. Matured oocytes were fertilized in vitro by capacitated spermatozoa. Randomly selected oocytes were observed by TLM for seven-to-eight days. Out of 76 developed embryos, 41 were morphologically normal, of which 15 reached the blastocyst stage. Of 35 abnormally developed embryos, 17 reached the blastocyst stage, of which six had single aberrations and 11 had multiple aberrations. The hatching rate (%) was 15.6% in normally cleaving embryos, 6.25% in embryos with single aberrations, and 3.33% in those with multiple aberrations. The present study reports the first results, found by using TLM, about the frequency of the morphological defects of feline embryos, their competence to reach the blastocyst stage, and their ability to hatch. View Full-Text
Keywords: cat; embryos; morphological disorders; time-lapse cat; embryos; morphological disorders; time-lapse
MDPI and ACS Style

Kij, B.; Kochan, J.; Nowak, A.; Niżański, W.; Prochowska, S.; Fryc, K.; Bugno-Poniewierska, M. Using Time Lapse Monitoring for Determination of Morphological Defect Frequency in Feline Embryos after in Vitro Fertilization (IVF). Animals 2020, 10, 3. https://doi.org/10.3390/ani10010003

AMA Style

Kij B, Kochan J, Nowak A, Niżański W, Prochowska S, Fryc K, Bugno-Poniewierska M. Using Time Lapse Monitoring for Determination of Morphological Defect Frequency in Feline Embryos after in Vitro Fertilization (IVF). Animals. 2020; 10(1):3. https://doi.org/10.3390/ani10010003

Chicago/Turabian Style

Kij, Barbara; Kochan, Joanna; Nowak, Agnieszka; Niżański, Wojciech; Prochowska, Sylwia; Fryc, Karolina; Bugno-Poniewierska, Monika. 2020. "Using Time Lapse Monitoring for Determination of Morphological Defect Frequency in Feline Embryos after in Vitro Fertilization (IVF)" Animals 10, no. 1: 3. https://doi.org/10.3390/ani10010003

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