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Article

The Impact of the Inoculation of Phosphate-Solubilizing Bacteria Pantoea agglomerans on Phosphorus Availability and Bacterial Community Dynamics of a Semi-Arid Soil

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Laboratoire de Microorganismes et Biomolécules Actives (LR03ES03), Facultédes Sciences de Tunis, Université Tunis El Manar, 2092 Tunis, Tunisia
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Higher Institute for Biotechnology (ISBST), LR Biotechnology and Bio-Geo Resources Valorization, University of Manouba, BVBGR-LR11ES31, Biotechpole Sidi Thabet, 2020 Ariana, Tunisia
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Laboratory of Systems Microbiology and Applied Genomics, Department of Environmental Engineering, University of Patras, 2 Seferi St., 30100 Agrinio, Greece
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Red Sea Research Center (RSRC), Biological and Environmental Sciences and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia
*
Authors to whom correspondence should be addressed.
Academic Editor: Eiko Kuramae
Microorganisms 2021, 9(8), 1661; https://doi.org/10.3390/microorganisms9081661
Received: 11 June 2021 / Revised: 2 July 2021 / Accepted: 4 July 2021 / Published: 4 August 2021
(This article belongs to the Special Issue Soil Microbiome: Biotic and Abiotic Interactions)
The bacterial genus Pantoea has been widely evaluated as promising bacteria to increase phosphorus (P) availability in soil. The aim of this study was to characterize the phosphate solubilizing (PS) activity of a Pantoea agglomerans strain and to evaluate the impact of its application in a semi-arid soil on phosphate availability and structure of the bacterial communities as a whole. An incubation experiment under close-to-natural soil environmental conditions was conducted for 15 days at 30 °C. High-throughput sequencing of the bacterial 16S rRNA gene was used to characterize and to compare the bacterial community structure of P. agglomerans-inoculated soil with non-inoculated control. Furthermore, a qPCR-based method was developed for detection and quantification of the functional genes related to the expression of mineral phosphate solubilization (MPS) phenotype in P. agglomerans. The results showed that in vitro solubilization of Ca3(PO4)2 by P. agglomerans strain was very efficient (980 mg/L), and it was associated with a drop in pH due to the secretion of gluconic acid; these changes were concomitant with the detection of gdh and pqqC genes. Moreover, P. agglomerans inoculum application significantly increased the content of available P in semi-arid soil by 69%. Metagenomic analyses showed that P. agglomerans treatment modified the overall edaphic bacterial community, significantly impacting its structure and composition. In particular, during P. agglomerans inoculation the relative abundance of bacteria belonging to Firmicutes (mainly Bacilli class) significantly increased, whereas the abundance of Actinobacteria together with Acidobacteria and Chloroflexi phyla decreased. Furthermore, genera known for their phosphate solubilizing activity, such as Aneurinibacillus, Lysinibacillus, Enterococcus, and Pontibacter, were exclusively detected in P. agglomerans-treated soil. Pearson’s correlation analysis revealed that changes in soil bacterial community composition were closely affected by soil characteristics, such as pH and available P. This study explores the effect of the inoculation of P. agglomerans on the bacterial community structure of a semi-arid soil. The effectiveness in improving the phosphate availability and modification in soil bacterial community suggested that P. agglomerans represent a promising environmental-friendly biofertilizer in arid and semi-arid ecosystems. View Full-Text
Keywords: available P; microbial inoculants; soil bacterial community composition; soil bacterial community structure; high-throughput sequencing available P; microbial inoculants; soil bacterial community composition; soil bacterial community structure; high-throughput sequencing
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MDPI and ACS Style

Saadouli, I.; Mosbah, A.; Ferjani, R.; Stathopoulou, P.; Galiatsatos, I.; Asimakis, E.; Marasco, R.; Daffonchio, D.; Tsiamis, G.; Ouzari, H.-I. The Impact of the Inoculation of Phosphate-Solubilizing Bacteria Pantoea agglomerans on Phosphorus Availability and Bacterial Community Dynamics of a Semi-Arid Soil. Microorganisms 2021, 9, 1661. https://doi.org/10.3390/microorganisms9081661

AMA Style

Saadouli I, Mosbah A, Ferjani R, Stathopoulou P, Galiatsatos I, Asimakis E, Marasco R, Daffonchio D, Tsiamis G, Ouzari H-I. The Impact of the Inoculation of Phosphate-Solubilizing Bacteria Pantoea agglomerans on Phosphorus Availability and Bacterial Community Dynamics of a Semi-Arid Soil. Microorganisms. 2021; 9(8):1661. https://doi.org/10.3390/microorganisms9081661

Chicago/Turabian Style

Saadouli, Ilhem, Amor Mosbah, Raoudha Ferjani, Panagiota Stathopoulou, Ioannis Galiatsatos, Elias Asimakis, Ramona Marasco, Daniele Daffonchio, George Tsiamis, and Hadda-Imene Ouzari. 2021. "The Impact of the Inoculation of Phosphate-Solubilizing Bacteria Pantoea agglomerans on Phosphorus Availability and Bacterial Community Dynamics of a Semi-Arid Soil" Microorganisms 9, no. 8: 1661. https://doi.org/10.3390/microorganisms9081661

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