Next Article in Journal
Clinical Characteristics Associated with the Development of Cystoid Macular Edema in Patients with Cytomegalovirus Retinitis
Previous Article in Journal
12/111phiA Prophage Domestication Is Associated with Autoaggregation and Increased Ability to Produce Biofilm in Streptococcus agalactiae
Article

Identification of Streptococcus thermophilus Genes Specifically Expressed under Simulated Human Digestive Conditions Using R-IVET Technology

1
EA 7488 Calbinotox Composés Alimentaires Biofonctionnalités & Risque Neurotoxique, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, France
2
UMR 454 MEDIS Microbiology, Digestive Environment and Health, Université Clermont Auvergne, INRAe, 63000 Clermont-Ferrand, France
3
UMR 5557 Microbial Ecology, Research Group on Bacterial Opportunistic Pathogens and Environment, CNRS, VetAgro Sup, 69280 Marcy L’Etoile, France
4
INRIA/IRISA, GenScale Bioinformatics Team, 35042 Rennes, France
*
Author to whom correspondence should be addressed.
Co-senior authors.
Academic Editor: Harsharn Gill
Microorganisms 2021, 9(6), 1113; https://doi.org/10.3390/microorganisms9061113
Received: 7 May 2021 / Revised: 18 May 2021 / Accepted: 19 May 2021 / Published: 21 May 2021
(This article belongs to the Section Food Microbiology)
Despite promising health effects, the probiotic status of Streptococcus thermophilus, a lactic acid bacterium widely used in dairy industry, requires further documentation of its physiological status during human gastrointestinal passage. This study aimed to apply recombinant-based in vivo technology (R-IVET) to identify genes triggered in a S. thermophilus LMD-9 reference strain under simulated digestive conditions. First, the R-IVET chromosomal cassette and plasmid genomic library were designed to positively select activated genes. Second, recombinant clones were introduced into complementary models mimicking the human gut, the Netherlands Organization for Applied Scientific Research (TNO) gastrointestinal model imitating the human stomach and small intestine, the Caco-2 TC7 cell line as a model of intestinal epithelium, and anaerobic batch cultures of human feces as a colon model. All inserts of activated clones displayed a promoter activity that differed from one digestive condition to another. Our results also showed that S. thermophilus adapted its metabolism to stressful conditions found in the gastric and colonic competitive environment and modified its surface proteins during adhesion to Caco-2 TC7 cells. Activated genes were investigated in a collection of S. thermophilus strains showing various resistance levels to gastrointestinal stresses, a first stage in the identification of gut resistance markers and a key step in probiotic selection. View Full-Text
Keywords: S. thermophilus; R-IVET; TIM-1 system; intestinal microbiota; adhesion S. thermophilus; R-IVET; TIM-1 system; intestinal microbiota; adhesion
Show Figures

Figure 1

MDPI and ACS Style

Uriot, O.; Kebouchi, M.; Lorson, E.; Galia, W.; Denis, S.; Chalancon, S.; Hafeez, Z.; Roux, E.; Genay, M.; Blanquet-Diot, S.; Dary-Mourot, A. Identification of Streptococcus thermophilus Genes Specifically Expressed under Simulated Human Digestive Conditions Using R-IVET Technology. Microorganisms 2021, 9, 1113. https://doi.org/10.3390/microorganisms9061113

AMA Style

Uriot O, Kebouchi M, Lorson E, Galia W, Denis S, Chalancon S, Hafeez Z, Roux E, Genay M, Blanquet-Diot S, Dary-Mourot A. Identification of Streptococcus thermophilus Genes Specifically Expressed under Simulated Human Digestive Conditions Using R-IVET Technology. Microorganisms. 2021; 9(6):1113. https://doi.org/10.3390/microorganisms9061113

Chicago/Turabian Style

Uriot, Ophélie, Mounira Kebouchi, Emilie Lorson, Wessam Galia, Sylvain Denis, Sandrine Chalancon, Zeeshan Hafeez, Emeline Roux, Magali Genay, Stéphanie Blanquet-Diot, and Annie Dary-Mourot. 2021. "Identification of Streptococcus thermophilus Genes Specifically Expressed under Simulated Human Digestive Conditions Using R-IVET Technology" Microorganisms 9, no. 6: 1113. https://doi.org/10.3390/microorganisms9061113

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop