Does Saccharomyces cerevisiae Require Specific Post-Translational Silencing against Leaky Translation of Hac1up?
Abstract
:1. Introduction
2. Materials and Methods
2.1. Retrieving the HAC1 Genes from 32 Yeast Species and Determining Their Introns
2.2. Determining the Putative 3′ Degron Sequence and Its Degree of Conservation in 32 Yeast Species
2.3. Reconstructing the Phylogenetic Relationship of 32 Yeast Species and Determining Relative Conservation at HAC1 Intronic and Exonic Regions
2.4. Retrieving and Processing S. cerevisiae Transcriptomic Data
2.5. Characterizing HAC1 Splicing Efficiency and Quantifying the Number of Translation Units Mapped to HAC1 Transcripts in S. cerevisiae
3. Results
3.1. Efficient HAC1 Splicing Occurs in Unfolded Protein Response (UPR)-Induced S. cerevisiae Cells
3.2. Translation of HAC1u Is Constitutive in Both UPR-Induced and Non-UPR S. cerevisiae Cells
3.3. The Putative Intron-Encoded Degron Is Conserved by Saccharomyces Yeasts but Not by Other Yeasts
3.4. The Degron Sequence Is the Most Conserved Segment at the HAC1 Intron
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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SRA Run | NEE 1 | NEI5 1 | NEI3 1 | p 2 | NTotal 3 | SE 4 | |
---|---|---|---|---|---|---|---|
UPR-induced (DTT) | SRR7265165 | 140 | 1 | 0 | 1 | 141 | 0.993 |
SRR7265166 | 90 | 0 | 0 | - | 90 | 1 | |
UPR-induced (TM) | SRR7265167 | 64 | 1 | 0 | 1 | 65 | 0.985 |
SRR7265168 | 42 | 2 | 0 | 1 | 44 | 0.955 |
SRA Run | NEE 1 | NEI5 1 | NEI3 1 | N_TargetHAC1u 2 | TIHAC1u 3 | |
---|---|---|---|---|---|---|
non-UPR | SRR7265151 | 5 | 134 | 137 | 126 | 128 |
SRR7265152 | 2 | 79 | 92 | 98 | 87 | |
UPR-induced | SRR7265153 | 178 | 73 | 95 | 126 | 91 |
SRR7265154 | 107 | 76 | 56 | 79 | 69 | |
SRR7265155 | 113 | 131 | 87 | 134 | 108 | |
SRR7265156 | 85 | 68 | 83 | 129 | 86 |
HAC1 Gene Region | Average Conservation Score | |
---|---|---|
Complete Alignment | Separate Alignment | |
First Exon | 0.207 | 0.197 |
Intron (Degron) | 0.256 | 0.308 |
Intron (Region downstream of Degron) | 0.017 | 0.049 |
Second Exon | 0.235 | 0.127 |
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Tehfe, A.; Roseshter, T.; Wei, Y.; Xia, X. Does Saccharomyces cerevisiae Require Specific Post-Translational Silencing against Leaky Translation of Hac1up? Microorganisms 2021, 9, 620. https://doi.org/10.3390/microorganisms9030620
Tehfe A, Roseshter T, Wei Y, Xia X. Does Saccharomyces cerevisiae Require Specific Post-Translational Silencing against Leaky Translation of Hac1up? Microorganisms. 2021; 9(3):620. https://doi.org/10.3390/microorganisms9030620
Chicago/Turabian StyleTehfe, Ali, Talia Roseshter, Yulong Wei, and Xuhua Xia. 2021. "Does Saccharomyces cerevisiae Require Specific Post-Translational Silencing against Leaky Translation of Hac1up?" Microorganisms 9, no. 3: 620. https://doi.org/10.3390/microorganisms9030620
APA StyleTehfe, A., Roseshter, T., Wei, Y., & Xia, X. (2021). Does Saccharomyces cerevisiae Require Specific Post-Translational Silencing against Leaky Translation of Hac1up? Microorganisms, 9(3), 620. https://doi.org/10.3390/microorganisms9030620