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Article

Development of a Genoserotyping Method for Salmonella Infantis Detection on the Basis of Pangenome Analysis

1
Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University, Yongin 17104, Korea
2
Department of Molecular Science and Technology, Ajou University, Suwon 16499, Korea
3
School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Korea
4
Department of Applied Chemistry and Biological Engineering, Ajou University, Suwon 16499, Korea
*
Authors to whom correspondence should be addressed.
Microorganisms 2021, 9(1), 67; https://doi.org/10.3390/microorganisms9010067
Received: 14 December 2020 / Revised: 24 December 2020 / Accepted: 25 December 2020 / Published: 29 December 2020
(This article belongs to the Special Issue Salmonella and Salmonellosis)
In recent years, Salmonella Infantis has become a predominant serovariant in clinical and poultry isolates, thereby imposing a substantial economic burden on both public health and the livestock industry. With the aim of coping with the steep increase in serovar Infantis prevalence, a polymerase chain reaction (PCR)-based rapid and accurate diagnostic assay was developed in this study through pangenome profiling of 60 Salmonella serovars. A gene marker, SIN_02055, was identified, which is present in the S. Infantis genome but not in the pangenome of the other serovars. Primers specific to SIN_02055 were used to accurately detect serovar Infantis, and to successfully differentiate Infantis from the other 59 serovars in real-time PCR with a R2 of 0.999 and an efficiency of 95.76%. The developed method was applied to 54 Salmonella strains belonging to eight dominant serovars, and distinguished Infantis from the other seven serovars with an accuracy of 100%. The diagnostic primer set also did not show false positive amplification with 32 strains from eight non-Salmonella bacterial species. This cost-effective and rapid method can be considered an alternative to the classic serotyping using antisera. View Full-Text
Keywords: Salmonella Infantis; genoserotyping; pangenome; real-time PCR Salmonella Infantis; genoserotyping; pangenome; real-time PCR
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MDPI and ACS Style

Yang, S.-M.; Baek, J.; Kim, E.; Kim, H.-B.; Ko, S.; Kim, D.; Yoon, H.; Kim, H.-Y. Development of a Genoserotyping Method for Salmonella Infantis Detection on the Basis of Pangenome Analysis. Microorganisms 2021, 9, 67. https://doi.org/10.3390/microorganisms9010067

AMA Style

Yang S-M, Baek J, Kim E, Kim H-B, Ko S, Kim D, Yoon H, Kim H-Y. Development of a Genoserotyping Method for Salmonella Infantis Detection on the Basis of Pangenome Analysis. Microorganisms. 2021; 9(1):67. https://doi.org/10.3390/microorganisms9010067

Chicago/Turabian Style

Yang, Seung-Min, Jiwon Baek, Eiseul Kim, Hyeon-Be Kim, Seyoung Ko, Donghyuk Kim, Hyunjin Yoon, and Hae-Yeong Kim. 2021. "Development of a Genoserotyping Method for Salmonella Infantis Detection on the Basis of Pangenome Analysis" Microorganisms 9, no. 1: 67. https://doi.org/10.3390/microorganisms9010067

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