Diversity of Gut Microbiota and Bifidobacterial Community of Chinese Subjects of Different Ages and from Different Regions
Abstract
:1. Introduction
2. Materials and Methods
2.1. Subject Recruitment and Fecal Sample Collection
2.2. DNA Extraction, PCR Amplification and High-Throughput Sequencing
2.3. Bioinformatics Analysis
2.4. Statistical Analysis
3. Results
3.1. Alpha-Diversity of Gut Microbiota of Subjects from Different Regions and across Age Groups
3.2. Beta-Diversity of Gut Microbiota of Subjects from Different Regions and across Age Groups
3.3. Composition of Gut Microbes of Subjects from Different Regions and Age Profiles
3.4. Functional Gene Composition of Gut Microbiota Predicted by PICRUSt
3.5. Alpha- and Beta-Diversity Analysis of Bifidobacterium Composition within Gut Microbiota of Subjects from Different Regions and across Age Groups
3.6. Composition of Bifidobacterium in Subjects from Different Regions and Age Groups
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Yang, B.; Yan, S.; Chen, Y.; Ross, R.P.; Stanton, C.; Zhao, J.; Zhang, H.; Chen, W. Diversity of Gut Microbiota and Bifidobacterial Community of Chinese Subjects of Different Ages and from Different Regions. Microorganisms 2020, 8, 1108. https://doi.org/10.3390/microorganisms8081108
Yang B, Yan S, Chen Y, Ross RP, Stanton C, Zhao J, Zhang H, Chen W. Diversity of Gut Microbiota and Bifidobacterial Community of Chinese Subjects of Different Ages and from Different Regions. Microorganisms. 2020; 8(8):1108. https://doi.org/10.3390/microorganisms8081108
Chicago/Turabian StyleYang, Bo, Shuang Yan, Yang Chen, R. Paul Ross, Catherine Stanton, Jianxin Zhao, Hao Zhang, and Wei Chen. 2020. "Diversity of Gut Microbiota and Bifidobacterial Community of Chinese Subjects of Different Ages and from Different Regions" Microorganisms 8, no. 8: 1108. https://doi.org/10.3390/microorganisms8081108