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SpoVG Is Necessary for Sporulation in Bacillus anthracis

State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Biotechnology, 20 Dongdajie Street, Fengtai District, Beijng 100071, China
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Microorganisms 2020, 8(4), 548;
Received: 3 March 2020 / Revised: 1 April 2020 / Accepted: 8 April 2020 / Published: 10 April 2020
(This article belongs to the Special Issue Bacillus: Molecular Considerations)
The Bacillus anthracis spore constitutes the infectious form of the bacterium, and sporulation is an important process in the organism’s life cycle. Herein, we show that disruption of SpoVG resulted in defective B. anthracis sporulation. Confocal microscopy demonstrated that a ΔspoVG mutant could not form an asymmetric septum, the first morphological change observed during sporulation. Moreover, levels of spoIIE mRNA were reduced in the spoVG mutant, as demonstrated using β-galactosidase activity assays. The effects on sporulation of the ΔspoVG mutation differed in B. anthracis from those in B. subtilis because of the redundant functions of SpoVG and SpoIIB in B. subtilis. SpoVG is highly conserved between B. anthracis and B. subtilis. Conversely, BA4688 (the protein tentatively assigned as SpoIIB in B. anthracis) and B. subtilis SpoIIB (SpoIIBBs) share only 27.9% sequence identity. On complementation of the B. anthracis ΔspoVG strain with spoIIBBs, the resulting strain pBspoIIBBsspoVG could not form resistant spores, but partially completed the prespore engulfment stage. In agreement with this finding, mRNA levels of the prespore engulfment gene spoIIM were significantly increased in strain pBspoIIBBsspoVG compared with the ΔspoVG strain. Transcription of the coat development gene cotE was similar in the pBspoIIBBsspoVG and ΔspoVG strains. Thus, unlike in B. subtilis, SpoVG appears to be required for sporulation in B. anthracis, which provides further insight into the sporulation mechanisms of this pathogen. View Full-Text
Keywords: Bacillus anthracis; spoVG; spoIIB; sporulation Bacillus anthracis; spoVG; spoIIB; sporulation
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MDPI and ACS Style

Chen, M.; Lyu, Y.; Feng, E.; Zhu, L.; Pan, C.; Wang, D.; Liu, X.; Wang, H. SpoVG Is Necessary for Sporulation in Bacillus anthracis. Microorganisms 2020, 8, 548.

AMA Style

Chen M, Lyu Y, Feng E, Zhu L, Pan C, Wang D, Liu X, Wang H. SpoVG Is Necessary for Sporulation in Bacillus anthracis. Microorganisms. 2020; 8(4):548.

Chicago/Turabian Style

Chen, Meng, Yufei Lyu, Erling Feng, Li Zhu, Chao Pan, Dongshu Wang, Xiankai Liu, and Hengliang Wang. 2020. "SpoVG Is Necessary for Sporulation in Bacillus anthracis" Microorganisms 8, no. 4: 548.

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