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Article

Effect of α-Hemolysin Producing E. coli in Two Different Mouse Strains in a DSS Model of Inflammatory Bowel Disease

1
Department of Bacteria, Parasites and Fungi, Statens Serum Institut, 2300 Copenhagen, Denmark
2
Department of Biology, University of Copenhagen, 2200 Copenhagen, Denmark
3
Division of Gastroenterology, BC Children’s Hospital, University of British Columbia, Vancouver, BC V6H 3N1, Canada
4
Institute of Molecular and Medical Biology, Roskilde University, 4000 Roskilde, Denmark
5
Department of Gastroenterology, Copenhagen University Hospital Hvidovre, 2650 Hvidovre, Denmark
6
Department of Clinical Microbiology, Copenhagen University Hospital Hvidovre, 2650 Hvidovre, Denmark
7
Department of Virus & Microbiological Special Diagnostics, Statens Serum Institute, 2300 Copenhagen, Denmark
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(12), 1971; https://doi.org/10.3390/microorganisms8121971
Received: 19 November 2020 / Accepted: 9 December 2020 / Published: 11 December 2020
Background: Phylogroup B2 Escherichia coli have been associated with ulcerative colitis (UC). In this study, we aimed to compare colonization with the UC-associated E. coli p19A in different mice strains, to investigate the role of alpha hemolysin in a UC mouse model. Methods: In this study, Sigirr −/− and C57BL/6 mice were chosen, and UC was induced by adding dextran sulfate sodium (DSS) to the drinking water. The mice were pre-treated with ciprofloxacin. p19A expressing luminescence and GFP, alpha-hemolysin knock out p19A-ΔhlyI II, and non-pathogenic lab E. coli DH10B were cultured in LB broth, and orally gavaged into the mice. Colonization with p19A WT was visualized using an in vivo imaging system. Results: p19A WT colonized the colon, ileum, Peyer’s patches, liver, and spleen of infected C57BL/6 and Sigirr −/− mice. A total of 99% of the p19A WT infected C57BL/6 mice and 29% of the p19A WT infected Sigirr −/− mice survived to the 4th post infection day. Conclusion: UC-associated E. coli p19A WT colonized the intestines of DSS-treated mice and caused extra-intestinal infection. Hemolysin is an important factor in this pathogenesis, since isogenic hemolysin mutants did not cause the same inflammation. View Full-Text
Keywords: inflammatory-bowel-disease; Sigirr −/− mice; C57BL/6-mice; Escherichia coli; alpha hemolysin inflammatory-bowel-disease; Sigirr −/− mice; C57BL/6-mice; Escherichia coli; alpha hemolysin
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MDPI and ACS Style

Mirsepasi-Lauridsen, H.C.; Struve, C.; Petersen, A.M.; Krogfelt, K.A. Effect of α-Hemolysin Producing E. coli in Two Different Mouse Strains in a DSS Model of Inflammatory Bowel Disease. Microorganisms 2020, 8, 1971. https://doi.org/10.3390/microorganisms8121971

AMA Style

Mirsepasi-Lauridsen HC, Struve C, Petersen AM, Krogfelt KA. Effect of α-Hemolysin Producing E. coli in Two Different Mouse Strains in a DSS Model of Inflammatory Bowel Disease. Microorganisms. 2020; 8(12):1971. https://doi.org/10.3390/microorganisms8121971

Chicago/Turabian Style

Mirsepasi-Lauridsen, Hengameh C., Carsten Struve, Andreas M. Petersen, and Karen A. Krogfelt. 2020. "Effect of α-Hemolysin Producing E. coli in Two Different Mouse Strains in a DSS Model of Inflammatory Bowel Disease" Microorganisms 8, no. 12: 1971. https://doi.org/10.3390/microorganisms8121971

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