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The Utilization of Linear Polylysine Coupled with Mechanic Forces to Extract Microbial DNA from Different Matrices

1
Gl-Biocontrol, 34830 Clapiers, France
2
Institute National de la Santé et de la Recherche Médicale, U1047, University Montpellier, UFR de Médecine, 30908 Nimes, France
3
Department of Microbiology, CHU Nimes, University Montpellier, 30029 Nimes, France
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EA7352 CHROME, University Nimes, Rue du Dr G. Salan, CEDEX 1, 30021 Nimes, France
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(12), 1901; https://doi.org/10.3390/microorganisms8121901
Received: 25 October 2020 / Revised: 26 November 2020 / Accepted: 27 November 2020 / Published: 30 November 2020
(This article belongs to the Section Molecular Microbiology and Immunology)
Molecular approaches are powerful tools that are used for medical or environmental diagnoses. However, the main limitations of such a tools are that they extract low levels of DNA and they do not remove the inhibitors of polymerase chain reaction (PCR). Although the use of polycation to complex and purify DNA has been described in the literature, elution often requires a high ionic strength or pH levels not compatible with molecular analyses. In this paper, we described a new process that is based on the complexation of DNA with linear polylysine, followed by capturing the complex by a cation exchange resin. The originality of the process consisted of using mechanic force to elute DNA from the complex. The extraction method showed several advantages when compared to existing methods, such as being compatible with pH levels that range from 5 to 11, as well as high levels of DNA recovery and elimination of PCR inhibitors from complex samples. This method was successfully applied to different types of samples, such as environmental samples, beverage samples, and medical samples. Furthermore, it was proven to be a good solution for removing PCR inhibitors and assuring good DNA recovery yield. View Full-Text
Keywords: linear polylysine; purification DNA; PCR inhibitors; complex samples linear polylysine; purification DNA; PCR inhibitors; complex samples
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MDPI and ACS Style

François, C.; Martinez, C.; Faye, C.; Pansu, N.; Dunyach-Remy, C.; Garrelly, L.; Roig, B.; Cadiere, A. The Utilization of Linear Polylysine Coupled with Mechanic Forces to Extract Microbial DNA from Different Matrices. Microorganisms 2020, 8, 1901. https://doi.org/10.3390/microorganisms8121901

AMA Style

François C, Martinez C, Faye C, Pansu N, Dunyach-Remy C, Garrelly L, Roig B, Cadiere A. The Utilization of Linear Polylysine Coupled with Mechanic Forces to Extract Microbial DNA from Different Matrices. Microorganisms. 2020; 8(12):1901. https://doi.org/10.3390/microorganisms8121901

Chicago/Turabian Style

François, Celia, Celia Martinez, Clement Faye, Nathalie Pansu, Catherine Dunyach-Remy, Laurent Garrelly, Benoit Roig, and Axelle Cadiere. 2020. "The Utilization of Linear Polylysine Coupled with Mechanic Forces to Extract Microbial DNA from Different Matrices" Microorganisms 8, no. 12: 1901. https://doi.org/10.3390/microorganisms8121901

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