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Morphology, Ultrastructure, and Mitochondrial Genome of the Marine Non-Photosynthetic Bicosoecid Cafileria marina Gen. et sp. nov.

1
Institute of Parasitology, Biology Centre, Czech Academy of Sciences, Branišovská 31, 370 05 České Budějovice, Czech Republic
2
Faculty of Science, University of South Bohemia, Branišovská 31, 370 05 České Budějovice, Czech Republic
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Microorganisms 2019, 7(8), 240; https://doi.org/10.3390/microorganisms7080240
Received: 26 June 2019 / Revised: 25 July 2019 / Accepted: 1 August 2019 / Published: 5 August 2019
(This article belongs to the Section Systems Microbiology)
In this paper, we describe a novel bacteriophagous biflagellate, Cafileria marina with two smooth flagellae, isolated from material collected from a rock surface in the Kvernesfjorden (Norway). This flagellate was characterized by scanning and transmission electron microscopy, fluorescence, and light microscopy. The sequence of the small subunit ribosomal RNA gene (18S) was used as a molecular marker for determining the phylogenetic position of this organism. Apart from the nuclear ribosomal gene, the whole mitochondrial genome was sequenced, assembled, and annotated. Morphological observations show that the newly described flagellate shares key ultrastructural characters with representatives of the family Bicosoecida (Heterokonta). Intriguingly, mitochondria of C. marina frequently associate with its nucleus through an electron-dense disc at the boundary of the two compartments. The function of this association remains unclear. Phylogenetic analyses corroborate the morphological data and place C. marina with other sequence data of representatives from the family Bicosoecida. We describe C. marina as a new species from a new genus in this family. View Full-Text
Keywords: heterotrophic nano-flagellate; heterokonta; bicosoecida; phylogeny; flagellar apparatus; new genus heterotrophic nano-flagellate; heterokonta; bicosoecida; phylogeny; flagellar apparatus; new genus
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    Doi: 10.5281/zenodo.3351652
    Link: http://doi.org/10.5281/zenodo.3351652
    Description: Figure S1. Phylogenetic tree constructed by the software PHYML is based on 18S sequences showing detailed branching with bootstraps as branch supports. Sequences of apicomplexans were used as an outgroup. Figure S2. Annotated mitochondrial (mt) genome of Cafileria marina. Total of the genome is 42,797bp and the genetic code is of type 4. In this type, the Trp code is UGA and UAA/UAG are translated as stops. Table S1. Table compares the lengths and procentual GC content of known mitochondrial genoms of stramenopiles with Cafileria marina. Table S2. Table provides comparison of selected stramenopiles mitochondrial genomes and Cafileria marina, including tRNA and rRNA genes, protein-coding genes encoded by mitochondrial genome.
MDPI and ACS Style

Jirsová, D.; Füssy, Z.; Richtová, J.; Gruber, A.; Oborník, M. Morphology, Ultrastructure, and Mitochondrial Genome of the Marine Non-Photosynthetic Bicosoecid Cafileria marina Gen. et sp. nov.. Microorganisms 2019, 7, 240.

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