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Open AccessArticle

Effects of Dietary Yogurt on the Healthy Human Gastrointestinal (GI) Microbiome

Biology Department, Youngstown State University, Youngstown, OH 44555, USA
Author to whom correspondence should be addressed.
Academic Editor: Giuseppe Comi
Microorganisms 2017, 5(1), 6;
Received: 15 December 2016 / Revised: 31 January 2017 / Accepted: 9 February 2017 / Published: 15 February 2017
(This article belongs to the Special Issue Microbes and Food)
The gastrointestinal (GI) tract performs key functions that regulate the relationship between the host and the microbiota. Research has shown numerous benefits of probiotic intake in the modulation of immune responses and human metabolic processes. However, unfavorable attention has been paid to temporal changes of the microbial composition and diversity of the GI tract. This study aimed to investigate the effects of yogurt consumption on the GI microbiome bacteria community composition, structure and diversity during and after a short-term period (42 days). We used a multi-approach combining classical fingerprinting techniques (T-RFLPs), Sanger analyses and Illumina MiSeq 16S rRNA gene amplicon sequencing to elucidate bacterial communities and Lactobacilli and Bifidobacteria populations within healthy adults that consume high doses of yogurt daily. Results indicated that overall GI microbial community and diversity was method-dependent, yet we found individual specific changes in bacterial composition and structure in healthy subjects that consumed high doses of yogurt throughout the study. View Full-Text
Keywords: 16S rDNA; gut microbiome; yogurt; diversity; next generation sequencing 16S rDNA; gut microbiome; yogurt; diversity; next generation sequencing
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MDPI and ACS Style

Lisko, D.J.; Johnston, G.P.; Johnston, C.G. Effects of Dietary Yogurt on the Healthy Human Gastrointestinal (GI) Microbiome. Microorganisms 2017, 5, 6.

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