Next Article in Journal
AfSwi6 Regulates the Stress Response, Chlamydospore Production, and Pathogenicity in the Nematode-Trapping Fungus Arthrobotrys flagrans
Next Article in Special Issue
Ingesting Stellera chamaejasme Significantly Impacts the Gastrointestinal Tract Bacterial Community and Diversity in Plateau Zokors (Eospalax baileyi)
Previous Article in Journal
Impact of Transgenic Maize Ruifeng125 on Diversity and Dynamics of Bacterial Community in Rhizosphere Soil
Previous Article in Special Issue
Comparative Analysis of Gut Microbiota between Captive and Wild Long-Tailed Gorals for Ex Situ Conservation
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Microorganisms
Volume 12
Issue 9
10.3390/microorganisms12091764
microorganisms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Structural and Functional Differences in Small Intestinal and Fecal Microbiota: 16S rRNA Gene Investigation in Rats

by
Xiao-Wei Sun
*,
Hong-Rui Li
,
Xiao-Lei Jin
,
Xiao Tang
,
Da-Wen Wang
,
Xiao Zhang
and
Jian-Gang Zhang
*
Pathology Institute, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China
*
Authors to whom correspondence should be addressed.
Microorganisms 2024, 12(9), 1764; https://doi.org/10.3390/microorganisms12091764
Submission received: 9 July 2024 / Revised: 13 August 2024 / Accepted: 22 August 2024 / Published: 25 August 2024
(This article belongs to the Special Issue Gut Microbiota: Metagenomics to Study Ecology)
Browse Figures
Review Reports Versions Notes

Abstract

To compare the differences in floral composition and functions between the two types of microbiota, ileal contents and feces were collected from Sprague Dawley (SD) rats fed in a conventional or specific-pathogen free (SPF) environment and rats fed a high-fat diet (HFD), and the V3–V4 region of the 16S ribosomal ribonucleic acid (rRNA) gene in these rats was then amplified and sequenced. Compared with feces, about 60% of the bacterial genera in the ileum were exclusive, with low abundance (operational taxonomic units (OTUs) < 1000). Of bacteria shared between the ileum and feces, a few genera were highly abundant (dominant), whereas most had low abundance (less dominant). The dominant bacteria differed between the ileum and feces. Ileal bacteria showed greater β-diversity, and the distance between in-group samples was nearer than that between paired ileum–feces samples. Moreover, the ileum shared various biomarkers and functions with feces (p < 0.05). The HFD and SPF conditions had a profound influence on α-diversity and abundance but not on the exclusive/shared features or β-diversity of samples. The present findings suggested that, under conventional circumstances, fecal bacteria can represent approximately 40% of the low abundant ileal bacterial genera and that dominant fecal bacteria failed to represent the ileal dominant flora. Moreover, fecal flora diversity does not reflect β-diversity in the ileum.
Keywords: ileum; feces; microbiota; 16S rRNA gene; sequencing; rats ileum; feces; microbiota; 16S rRNA gene; sequencing; rats

Share and Cite

MDPI and ACS Style

Sun, X.-W.; Li, H.-R.; Jin, X.-L.; Tang, X.; Wang, D.-W.; Zhang, X.; Zhang, J.-G. Structural and Functional Differences in Small Intestinal and Fecal Microbiota: 16S rRNA Gene Investigation in Rats. Microorganisms 2024, 12, 1764. https://doi.org/10.3390/microorganisms12091764

AMA Style

Sun X-W, Li H-R, Jin X-L, Tang X, Wang D-W, Zhang X, Zhang J-G. Structural and Functional Differences in Small Intestinal and Fecal Microbiota: 16S rRNA Gene Investigation in Rats. Microorganisms. 2024; 12(9):1764. https://doi.org/10.3390/microorganisms12091764

Chicago/Turabian Style

Sun, Xiao-Wei, Hong-Rui Li, Xiao-Lei Jin, Xiao Tang, Da-Wen Wang, Xiao Zhang, and Jian-Gang Zhang. 2024. "Structural and Functional Differences in Small Intestinal and Fecal Microbiota: 16S rRNA Gene Investigation in Rats" Microorganisms 12, no. 9: 1764. https://doi.org/10.3390/microorganisms12091764

APA Style

Sun, X.-W., Li, H.-R., Jin, X.-L., Tang, X., Wang, D.-W., Zhang, X., & Zhang, J.-G. (2024). Structural and Functional Differences in Small Intestinal and Fecal Microbiota: 16S rRNA Gene Investigation in Rats. Microorganisms, 12(9), 1764. https://doi.org/10.3390/microorganisms12091764

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop