Impacts of Oak Mulch Amendments on Rhizosphere Microbiome of Citrus Trees Grown in Florida Flatwood Soils
Abstract
:1. Introduction
2. Materials and Methods
2.1. Site Description and Plant Materials
2.2. Leaf Nutrient Analysis
2.3. Soil Nutrient, Temperature, and Moisure Analysis
2.4. Sampling of Rhizosophere Soil and DNA Isolation
2.5. DNA Quantification and PCR Amplification of Rhizophere Soil Samples
2.6. Sequencing Analysis and Taxonomic Assignment of Microorganisms Associated with the Rhizosphere Soil Samples
2.7. Plant and Soil Data Statistical Analysis
2.8. Rhizosphere Composition Statistical Analysis
3. Results
3.1. Impact of Oak Mulch on Soil and Leaf Nutrient Concentrations
3.2. Impact of Oak Mulch on Soil Characteristics
3.3. Impact of Oak Mulch on Rhizosphere Bacterial Communities
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Bacteria | Oak Mulch | Control | p-Value |
---|---|---|---|
Rhizobiales | 15.78% | 11.20% | 0.006 |
Gemmatimonadales | 2.26% | 4.18% | 0.05 |
Vicinamibacterales | 5.43% | 4.01% | 0.05 |
Pirellulales | 1.13% | 2.82% | 0.03 |
Nitrospirales | 1.13% | 2.14% | 0.05 |
Frankiales | 0.34% | 1.12% | 0.003 |
Haliangiales | 0.67% | 1.00% | 0.03 |
Planctomycetales | 1.60% | 0.97% | 0.01 |
Latescibacterota | 0.29% | 0.75% | 0.003 |
Reyranellales | 0.73% | 0.32% | 0.03 |
Nitrosotaleales | 0.03% | 0.32% | 0.05 |
Caldilineales | 0.35% | 0.19% | 0.02 |
Entotheonellales | 0.34% | 0.19% | 0.01 |
Thermoanaerobaculales | 0.31% | 0.17% | 0.05 |
Flavobacteriales | 0.27% | 0.14% | 0.03 |
Bdellovibrionales | 0.24% | 0.12% | 0.003 |
Kapabacteriales | 0.05% | 0.10% | 0.04 |
Kallotenuales | 0.03% | 0.09% | 0.04 |
Pseudomonadales | 0.26% | 0.07% | 0.006 |
Azospirillales | 0.01% | 0.04% | 0.03 |
Babeliales | 0.32% | 0.02% | 0.02 |
Bacteria | Oak Mulch | Control | p-Value |
---|---|---|---|
Rhizobiales | 14.28% | 10.65% | 0.05 |
Burkholderiales | 5.74% | 7.36% | 0.05 |
Gemmatimonadales | 2.28% | 4.70% | 0.006 |
Rokubacteriales | 1.28% | 3.04% | 0.02 |
Nitrospirales | 1.15% | 2.38% | 0.02 |
Solirubrobacterales | 3.48% | 2.25% | 0.02 |
Latescibacterota | 0.23% | 0.94% | 0.02 |
Tepidisphaerales | 0.06% | 0.34% | 0.02 |
Reyranellales | 0.67% | 0.34% | 0.05 |
Diplorickettsiales | 0.48% | 0.23% | 0.02 |
Dadabacteriales | 0.07% | 0.22% | 0.05 |
Fimbriimonadales | 0.07% | 0.19% | 0.05 |
Bdellovibrionales | 0.28% | 0.16% | 0.05 |
Myxococcales | 0.28% | 0.17% | 0.04 |
Kapabacteriales | 0.06% | 0.13% | 0.05 |
Longimicrobiales | 0.02% | 0.07% | 0.05 |
Streptosporangiales | 0.15% | 0.06% | 0.04 |
Deinococcales | 0.04% | 0.005% | 0.01 |
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Santiago, J.M.; Hallman, L.M.; Fox, J.-P.; Pitino, M.; Shatters, R.G., Jr.; Cano, L.M.; Rossi, L. Impacts of Oak Mulch Amendments on Rhizosphere Microbiome of Citrus Trees Grown in Florida Flatwood Soils. Microorganisms 2023, 11, 2764. https://doi.org/10.3390/microorganisms11112764
Santiago JM, Hallman LM, Fox J-P, Pitino M, Shatters RG Jr., Cano LM, Rossi L. Impacts of Oak Mulch Amendments on Rhizosphere Microbiome of Citrus Trees Grown in Florida Flatwood Soils. Microorganisms. 2023; 11(11):2764. https://doi.org/10.3390/microorganisms11112764
Chicago/Turabian StyleSantiago, John M., Lukas M. Hallman, John-Paul Fox, Marco Pitino, Robert G. Shatters, Jr., Liliana M. Cano, and Lorenzo Rossi. 2023. "Impacts of Oak Mulch Amendments on Rhizosphere Microbiome of Citrus Trees Grown in Florida Flatwood Soils" Microorganisms 11, no. 11: 2764. https://doi.org/10.3390/microorganisms11112764