Guardians of the Herd: Molecular Surveillance of Tick Vectors Uncovers Theileriosis Perils in Large Ruminants
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Area and Collection of Ticks
2.2. Collection of Blood
2.3. Identification and Dissections of Ticks
2.4. DNA Extraction and Amplification
2.5. Statistical Analyses
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Levels | Positive | Prevalence (%) | Odds Ratio | p-Value | 95% Confidence Interval |
---|---|---|---|---|---|
Hyalomma anatolicum | 136 | 35.4 | 1.39 | 0.000 | 41.56–45.54 |
Rhipicephalus (Boophilus) microplus | 116 | 30.2 | 1.18 | 0.001 | 27.41–31.19 |
Rhipicephalus sanguineus | 96 | 25 | - | - | 22.20–25.66 |
Variables | Levels | Examined | Positive | Odds Ratio | p-Value | 95% Confidence Interval |
---|---|---|---|---|---|---|
Host | Non-host | 116 | 42 | - | - | 21.61–51.17 |
Buffalo ticks | 66 | 24 | 2.47 | 0.029 | 20.30–52.51 | |
Cattle ticks | 70 | 60 | 1.37 | 0.000 | 70.03–93.46 | |
Sex and Infestation Status | Male ticks (infesting) | 62 | 36 | 4.49 | 0.007 | 41.24–73.22 |
Female ticks (infesting) | 74 | 48 | 5.14 | 0.003 | 47.50–77.76 | |
Male ticks (questing) | 62 | 8 | - | - | 03.13–27.14 | |
Female ticks (questing) | 54 | 34 | 4.76 | 0.006 | 42.74–78.31 | |
Ecological Variable | Non-riverine | 126 | 40 | - | - | 20.06–42.85 |
Riverine | 126 | 86 | 2.04 | 0.000 | 55.13–77.72 |
Variables | Levels | Animal Examined | Positive | Prevalence (%) | Odds Ratio | p-Value | 95% Confidence Interval |
---|---|---|---|---|---|---|---|
Species | Cattle | 192 | 83 | 43.2 | 1.12 | 0.001 | 41.58–45.26 |
Buffalo | 192 | 55 | 28.6 | 25.79–31.25 | |||
Cattle breed | Friesian | 38 | 22 | 57.9 | 1.42 | 0.002 | 54.45–60.38 |
Jersey | 69 | 31 | 44.9 | 2.34 | 0.043 | 42.16–47.36 | |
Sahiwal | 85 | 30 | 35.3 | - | - | 33.16–37.51 | |
Buffalo breeds | Nili Ravi | 114 | 20 | 17.5 | 2.18 | 0.000 | 15.38–19.04 |
Kundi | 78 | 35 | 44.9 | 41.65–46.64 | |||
Cattle sex | Male | 46 | 13 | 28.3 | 2.19 | 0.000 | 25.78–30.51 |
Female | 146 | 70 | 47.9 | 45.62–50.25 | |||
Buffalo sex | Male | 37 | 6 | 16.2 | 1.86 | 0.000 | 14.67–20.64 |
Female | 155 | 49 | 31.6 | 29.38–33.34 | |||
Cattle age | Young | 70 | 39 | 55.7 | 1.49 | 0.000 | 53.44–58.41 |
Adult | 122 | 44 | 36.1 | 33.08–38.05 | |||
Buffalo age | Young | 62 | 25 | 40.3 | 1.37 | 0.000 | 38.37–42.34 |
Adult | 130 | 30 | 23.1 | 21.72–25.79 | |||
Cattle | Riverine | 92 | 48 | 52.2 | 1.98 | 0.000 | 49.09–54.36 |
Non-riverine | 100 | 35 | 35 | 32.37–37.43 | |||
Buffalo | Riverine | 105 | 38 | 36.2 | 2.12 | 0.000 | 34.94–38.91 |
Non-riverine | 87 | 17 | 19.5 | 17.74–21.57 |
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Sajid, M.S.; Iqbal, A.; Rizwan, H.M.; Kausar, A.; Tahir, U.B.; Younus, M.; Maqbool, M.; Siddique, R.M.; Fouad, D.; Ataya, F.S. Guardians of the Herd: Molecular Surveillance of Tick Vectors Uncovers Theileriosis Perils in Large Ruminants. Microorganisms 2023, 11, 2684. https://doi.org/10.3390/microorganisms11112684
Sajid MS, Iqbal A, Rizwan HM, Kausar A, Tahir UB, Younus M, Maqbool M, Siddique RM, Fouad D, Ataya FS. Guardians of the Herd: Molecular Surveillance of Tick Vectors Uncovers Theileriosis Perils in Large Ruminants. Microorganisms. 2023; 11(11):2684. https://doi.org/10.3390/microorganisms11112684
Chicago/Turabian StyleSajid, Muhammad Sohail, Asif Iqbal, Hafiz Muhammad Rizwan, Asma Kausar, Urfa Bin Tahir, Muhammad Younus, Mahvish Maqbool, Rao Muhammad Siddique, Dalia Fouad, and Farid Shokry Ataya. 2023. "Guardians of the Herd: Molecular Surveillance of Tick Vectors Uncovers Theileriosis Perils in Large Ruminants" Microorganisms 11, no. 11: 2684. https://doi.org/10.3390/microorganisms11112684