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Article

Biofilm Formation and Expression of Virulence Genes of Microorganisms Grown in Contact with a New Bioactive Glass

1
Human Exposome and Infectious Diseases Network—HEID, School of Nursing of Ribeirão Preto, University of São Paulo, Bandeirantes Avenue 3900, Ribeirão Preto 14040-904, São Paulo, Brazil
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Department of Dental Materials and Prostheses, School of Dentistry of Ribeirão Preto, University of São Paulo, Café Avenue S/N, Ribeirão Preto 14040-904, São Paulo, Brazil
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Vitreous Materials Laboratory—LaMaV, Department of Materials Engineering, Federal University of São Carlos, Rod. Washington Luiz km 235, São Carlos 13565-905, São Paulo, Brazil
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Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Café Avenue S/N, Ribeirão Preto 14040-904, São Paulo, Brazil
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Department of Orthopedic Surgery, Experimental Orthopedics, Medical University of Innsbruck, Peter‒Mayr-Strasse 4b, 6020 Innsbruck, Austria
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(11), 927; https://doi.org/10.3390/pathogens9110927
Received: 20 October 2020 / Revised: 3 November 2020 / Accepted: 5 November 2020 / Published: 10 November 2020
Bioactive glass F18 (BGF18), a glass containing SiO2–Na2O–K2O–MgO–CaO–P2O5, is highly effective as an osseointegration buster agent when applied as a coating in titanium implants. Biocompatibility tests using this biomaterial exhibited positive results; however, its antimicrobial activity is still under investigation. In this study we evaluated biofilm formation and expression of virulence-factor-related genes in Candida albicans, Staphylococcus epidermidis, and Pseudomonas aeruginosa grown on surfaces of titanium and titanium coated with BGF18. C. albicans, S. epidermidis, and P. aeruginosa biofilms were grown on specimens for 8, 24, and 48 h. After each interval, the pH was measured and the colony-forming units were counted for the biofilm recovery rates. In parallel, quantitative real-time polymerase chain reactions were carried out to verify the expression of virulence-factor-related genes. Our results showed that pH changes of the culture in contact with the bioactive glass were merely observed. Reduction in biofilm formation was not observed at any of the studied time. However, changes in the expression level of genes related to virulence factors were observed after 8 and 48 h of culture in BGF18. BGF18 coating did not have a clear inhibitory effect on biofilm growth but promoted the modulation of virulence factors. View Full-Text
Keywords: biofilms; gene expression; Candida albicans; Pseudomonas aeruginosa; Staphylococcus epidermidis; bioactive glass biofilms; gene expression; Candida albicans; Pseudomonas aeruginosa; Staphylococcus epidermidis; bioactive glass
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MDPI and ACS Style

Oliveira, V.d.C.; Souza, M.T.; Zanotto, E.D.; Watanabe, E.; Coraça-Huber, D. Biofilm Formation and Expression of Virulence Genes of Microorganisms Grown in Contact with a New Bioactive Glass. Pathogens 2020, 9, 927. https://doi.org/10.3390/pathogens9110927

AMA Style

Oliveira VdC, Souza MT, Zanotto ED, Watanabe E, Coraça-Huber D. Biofilm Formation and Expression of Virulence Genes of Microorganisms Grown in Contact with a New Bioactive Glass. Pathogens. 2020; 9(11):927. https://doi.org/10.3390/pathogens9110927

Chicago/Turabian Style

Oliveira, Viviane d.C., Marina T. Souza, Edgar D. Zanotto, Evandro Watanabe, and Débora Coraça-Huber. 2020. "Biofilm Formation and Expression of Virulence Genes of Microorganisms Grown in Contact with a New Bioactive Glass" Pathogens 9, no. 11: 927. https://doi.org/10.3390/pathogens9110927

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