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Pathogens 2018, 7(3), 75; https://doi.org/10.3390/pathogens7030075

In Vitro Activity of Newer and Conventional Antimicrobial Agents, Including Fosfomycin and Colistin, against Selected Gram-Negative Bacilli in Kuwait

1
Microbiology Unit, Department of Laboratories, Farwaniya Hospital, Farwaniya 81004, Kuwait
2
Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait City 13110, Kuwait
3
Center of Anti-Infective Research, Hartford Hospital, Hartford, CT 06102, USA
*
Author to whom correspondence should be addressed.
Received: 20 June 2018 / Revised: 6 September 2018 / Accepted: 11 September 2018 / Published: 17 September 2018
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Abstract

Limited data are available on susceptibilities of these organisms to some of the recently made accessible antimicrobial agents. The in vitro activities of newer antibiotics, such as, ceftolozane/tazobactam (C/T) and ceftazidime/avibactam (CZA) along with some “older” antibiotics, for example fosfomycin (FOS) and colistin (CL) were determined against selected strains (resistant to 3 antimicrobial agents) of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Minimum inhibitory concentrations (MIC) were determined by Clinical and Laboratory Standards Institute microbroth dilution. 133 isolates: 46 E. coli, 39 K. pneumoniae, and 48 P. aeruginosa were tested. Results showed that E. coli isolates with MIC50/90, 0.5/1 μ g / mL for CL; 4/32 μ g / mL for FOS; 0.25/32 μ g / mL for C/T; 0.25/8 μ g / mL for CZA, exhibited susceptibility rates of 95.7%, 97.8%, 76.1%, and 89.1%, respectively. On the other hand, K. pneumoniae strains with MIC50/90, 0.5/1 μ g / mL for CL; 256/512 μ g / mL for FOS; 2/128 μ g / mL for C/T; 0.5/128 μ g / mL for CZA showed susceptibility rates of 92.3%, 7.7%, 51.3%, and 64.1%, respectively. P. aeruginosa isolates with MIC50/90, 1/1 μ g / mL for CL; 128/128 μ g / mL for C/T; 32/64 μ g / mL for CZA presented susceptibility rates of 97.9%, 33.3%, and 39.6%, respectively. Higher MICs were demonstrated against most of the antibiotics. However, CL retained efficacy at low MICs against most of the isolates tested. View Full-Text
Keywords: in vitro activity; Gram-negative bacilli; antimicrobial agents; ceftolozane/tazobactam; ceftazidime/avibactam; fosfomycin; colistin in vitro activity; Gram-negative bacilli; antimicrobial agents; ceftolozane/tazobactam; ceftazidime/avibactam; fosfomycin; colistin
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Alfouzan, W.; Dhar, R.; Nicolau, D.P. In Vitro Activity of Newer and Conventional Antimicrobial Agents, Including Fosfomycin and Colistin, against Selected Gram-Negative Bacilli in Kuwait. Pathogens 2018, 7, 75.

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