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Article

Plasmepsin-like Aspartyl Proteases in Babesia

1
Institute of Parasitology, Biology Centre, Academy of Sciences of the Czech Republic, Branišovská 1160/31, CZ-37005 České Budějovice, Czech Republic
2
Faculty of Science, University of South Bohemia in České Budějovice, Branišovská 1760c, CZ-37005 České Budějovice, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editors: Estrella Montero, Jeremy Gray, Cheryl Ann Lobo and Luis Miguel González
Pathogens 2021, 10(10), 1241; https://doi.org/10.3390/pathogens10101241
Received: 3 August 2021 / Revised: 17 September 2021 / Accepted: 22 September 2021 / Published: 26 September 2021
(This article belongs to the Special Issue Babesia and Human Babesiosis)
Apicomplexan genomes encode multiple pepsin-family aspartyl proteases (APs) that phylogenetically cluster to six independent clades (A to F). Such diversification has been powered by the function-driven evolution of the ancestral apicomplexan AP gene and is associated with the adaptation of various apicomplexan species to different strategies of host infection and transmission through various invertebrate vectors. To estimate the potential roles of Babesia APs, we performed qRT-PCR-based expressional profiling of Babesia microti APs (BmASP2, 3, 5, 6), which revealed the dynamically changing mRNA levels and indicated the specific roles of individual BmASP isoenzymes throughout the life cycle of this parasite. To expand on the current knowledge on piroplasmid APs, we searched the EuPathDB and NCBI GenBank databases to identify and phylogenetically analyse the complete sets of APs encoded by the genomes of selected Babesia and Theileria species. Our results clearly determine the potential roles of identified APs by their phylogenetic relation to their homologues of known function—Plasmodium falciparum plasmepsins (PfPM I–X) and Toxoplasma gondii aspartyl proteases (TgASP1–7). Due to the analogies with plasmodial plasmepsins, piroplasmid APs represent valuable enzymatic targets that are druggable by small molecule inhibitors—candidate molecules for the yet-missing specific therapy for babesiosis. View Full-Text
Keywords: aspartyl protease; plasmepsin; apicomplexa; piroplasmida; Babesia aspartyl protease; plasmepsin; apicomplexa; piroplasmida; Babesia
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Figure 1

  • Externally hosted supplementary file 1
    Doi: 10.17632/ds3f2j32ny.1
    Link: http://dx.doi.org/10.17632/ds3f2j32ny.1.
    Description: The multiple alignmet data used to construct the maximum likelihood tree in figure 2
MDPI and ACS Style

Šnebergerová, P.; Bartošová-Sojková, P.; Jalovecká, M.; Sojka, D. Plasmepsin-like Aspartyl Proteases in Babesia. Pathogens 2021, 10, 1241. https://doi.org/10.3390/pathogens10101241

AMA Style

Šnebergerová P, Bartošová-Sojková P, Jalovecká M, Sojka D. Plasmepsin-like Aspartyl Proteases in Babesia. Pathogens. 2021; 10(10):1241. https://doi.org/10.3390/pathogens10101241

Chicago/Turabian Style

Šnebergerová, Pavla, Pavla Bartošová-Sojková, Marie Jalovecká, and Daniel Sojka. 2021. "Plasmepsin-like Aspartyl Proteases in Babesia" Pathogens 10, no. 10: 1241. https://doi.org/10.3390/pathogens10101241

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