Effects of Endosymbiont Disruption on the Nutritional Dynamics of the Pea Aphid Acyrthosiphon pisum
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plants and Insects
2.2. Antibiotic Screening for Endosymbiont Disruption in the Pea Aphid
2.3. Effects of Buchnera Disruption on the Growth of Pea Aphid
2.4. Quantification of the Dynamic Changes of Pea Aphid Nutrition
2.4.1. Protein Detection
2.4.2. Carbohydrate Detection
2.4.3. Lipid Detection
2.5. Statistical Analysis
3. Results
3.1. Endosymbiont Disruption in Pea Aphids
3.2. Effects of Buchnera Disruption on the Growth of Pea Aphid
3.3. Quantification of the Dynamic Changes of Proteins
3.4. Quantification for the Dynamic Changes of Carbohydrates
3.5. Quantification of the Dynamic Changes to Lipids
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Symbiotic Pea Aphids | Aposymbiotic Pea Aphids | ||
---|---|---|---|
Length (μm) | 3333.26 ± 70.19 | 1788.64 ± 44.07 * | t = 28.314, df = 58, p < 0.05 |
Width (μm) | 1411.80 ± 37.30 | 705.23 ± 32.59 * | t = 16.447, df = 58, p < 0.05 |
Weight (mg) | 3.525 ± 0.125 | 1.238 ± 0.132 * | t = 27.691, df = 58, p < 0.05 |
Number of offspring | 60.57 ± 1.98 | 0 * | t = 121.911, df = 58, p < 0.01 |
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Lv, N.; Wang, L.; Sang, W.; Liu, C.-Z.; Qiu, B.-L. Effects of Endosymbiont Disruption on the Nutritional Dynamics of the Pea Aphid Acyrthosiphon pisum. Insects 2018, 9, 161. https://doi.org/10.3390/insects9040161
Lv N, Wang L, Sang W, Liu C-Z, Qiu B-L. Effects of Endosymbiont Disruption on the Nutritional Dynamics of the Pea Aphid Acyrthosiphon pisum. Insects. 2018; 9(4):161. https://doi.org/10.3390/insects9040161
Chicago/Turabian StyleLv, Ning, Lei Wang, Wen Sang, Chang-Zhong Liu, and Bao-Li Qiu. 2018. "Effects of Endosymbiont Disruption on the Nutritional Dynamics of the Pea Aphid Acyrthosiphon pisum" Insects 9, no. 4: 161. https://doi.org/10.3390/insects9040161