Possible Regulation of Larval Juvenile Hormone Titers in Bombyx mori by BmFAMeT6
Abstract
:Simple Summary
Abstract
1. Introduction
1.1. Synthesis Pathway of Juvenile Hormone
1.2. Role of FAMeT in Juvenile Hormone Synthesis
2. Materials and Methods
2.1. Experimental Insects and Feeding
2.2. Fluorescence Screening of Transgenic Silkworm and Insertion Site Detection of Overexpressed Genes
2.3. Molecular Detection of Transgenic Bombyx Mori
2.4. Detection of Farnesoic Acid (FA), Methyl Farnesoate (MF), Juvenile Hormone I and Ⅱ (JH Ⅰ and JH Ⅱ)
2.5. Construction of the Recombinant Expression Vector
2.6. Injection of Exogenous Farnesoic Acid
2.7. Data Processing
3. Results
3.1. Insertion Sites of Overexpressed Genes
3.2. Identification of the Overexpression Silkworm Strain
3.3. Construction and Verification of the Knockout Silkworm Strain
3.4. Observation of Phenotype in Transgenic Individuals
3.5. Analysis of FA and JH Related Indexes in Transgenic Individuals
3.6. Effect of Exogenous Farnesoic Acid on JH Titer
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Yu, Y.; Li, T.; Guo, M.; Xiong, R.; Yan, D.; Chen, P. Possible Regulation of Larval Juvenile Hormone Titers in Bombyx mori by BmFAMeT6. Insects 2023, 14, 644. https://doi.org/10.3390/insects14070644
Yu Y, Li T, Guo M, Xiong R, Yan D, Chen P. Possible Regulation of Larval Juvenile Hormone Titers in Bombyx mori by BmFAMeT6. Insects. 2023; 14(7):644. https://doi.org/10.3390/insects14070644
Chicago/Turabian StyleYu, Yang, Tian Li, Meiwei Guo, Rong Xiong, Dongshen Yan, and Ping Chen. 2023. "Possible Regulation of Larval Juvenile Hormone Titers in Bombyx mori by BmFAMeT6" Insects 14, no. 7: 644. https://doi.org/10.3390/insects14070644