Pharmacological Properties and Function of the PxOctβ3 Octopamine Receptor in Plutella xylostella (L.)
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Insects
2.2. Total RNA Extraction and cDNA Synthesis
2.3. Cloning of the Full-Length PxOctβ3 ORF Sequence
2.4. Bioinformatic Analysis of the PxOctβ3
2.5. Analysis of the Pattern of Expression of PxOctβ3
2.6. Construction of a Cell Line That Stably Expresses PxOctβ3
2.7. Cyclic AMP (cAMP) Assays
2.8. Synthesis and Microinjection of dsRNA
2.9. Data Analysis
3. Results
3.1. Molecular Cloning and Sequence Analysis
3.2. Pattern of Expression of PxOctβ3
3.3. Pharmacological Property Analysis of PxOctβ3
3.4. RNA Interference and Pupation Rate Determination after RNAi
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Primer Name | Primer Sequences | Purpose of Primers |
---|---|---|
Octβ3R_XhoI_F | CTCGAGCCACCATGACCCATGGCACGCTGC | DNA cloning |
Octβ3R_PstI_R | TCTCTATATACTGCAGCACAAACTGTGC | |
Octβ3_qF | CGTCTAGCGTATCGTTTTGGAT | Quantitative RT-PCR |
Octβ3_qR | GTGGAGGTAGTGTGAATGGTGT | |
Rpl32_qF | TGCCCAACATTGGTTACGG | |
Rpl32_qR | ACGATGGCCTTGCGCTTC | |
dsOctβ3_F | TAATACGACTCACTATAGGGCCATCGTCAGACCCTTAG | RNAi |
dsOctβ3_R | TAATACGACTCACTATAGGCGTGCTGTAGTTCGGACTTAT | |
Octβ3_ygF | CTGCCCTTCTTCCTGTGG | |
Octβ3_ygR | GTGCGTTATTAGCGTCCTT |
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Zhu, H.; Liu, Z.; Ma, H.; Zheng, W.; Liu, J.; Zhou, Y.; Man, Y.; Zhou, X.; Zeng, A. Pharmacological Properties and Function of the PxOctβ3 Octopamine Receptor in Plutella xylostella (L.). Insects 2022, 13, 735. https://doi.org/10.3390/insects13080735
Zhu H, Liu Z, Ma H, Zheng W, Liu J, Zhou Y, Man Y, Zhou X, Zeng A. Pharmacological Properties and Function of the PxOctβ3 Octopamine Receptor in Plutella xylostella (L.). Insects. 2022; 13(8):735. https://doi.org/10.3390/insects13080735
Chicago/Turabian StyleZhu, Hang, Zheming Liu, Haihao Ma, Wei Zheng, Jia Liu, Yong Zhou, Yilong Man, Xiaomao Zhou, and Aiping Zeng. 2022. "Pharmacological Properties and Function of the PxOctβ3 Octopamine Receptor in Plutella xylostella (L.)" Insects 13, no. 8: 735. https://doi.org/10.3390/insects13080735