Duration of Low Temperature Exposure Affects Egg Hatching of the Colorado Potato Beetle and Emergence of Overwintering Adults
Round 1
Reviewer 1 Report
This paper provides a straightforward study assessing the effect of cold exposure on the Colorado potato beetle. The general methods are sound, but I do have one concern with the analysis (see comments below pertaining to figure 2). The results are clear, and the discussion uses good science to interpret and discuss the results.
There are several run-on sentences that make reading the text a little difficult at times. The content is generally good, but should be broken up into more individual sentences at times (see some specific comments below for examples). There are also several spelling and other grammatical errors in the paper. I recommend a thorough read-through from an outside party.
Specific comments:
Simple summary – check to make sure you have periods and not commas. For example, in line 11, there should be a period after “China”, or “More” should not be capitalized.
Line 41: change “fed” to “feed”.
Lines 40-45: break this up into at least two sentences. This is a run on sentence.
Lines 61-62: this is not really a sentence. Are you saying that global warming causes frequent extreme climatic events and abnormal temperature fluctuations in the spring? Please clarify the sentence.
Lines 63-65: rewrite as “Because insects are ectotherms, there growth, development and survival are affected by environmental factors, such as temperature, humidity, and precipitation.
Lines 63-65: while the statement is not wrong, endotherms are also affected by all these factors. Maybe add something stating why ectotherms are more affected by these factors? Or why they are sensitive to these factors? That they do not internally regulate temperature like endotherms?
Line 71: what is meant by “overwintering”. I know what overwintering is generally, and diapause is one way organisms overwinter. This seems a bit vague here.
Lines 73-77: this is a run-on sentence. Break up for clarity.
Methods:
-how did you record the temperature of the refrigerator? Some type of thermometer/temperature logger?
Data analysis:
-I have concerns with the statistical analysis when looking at figure 2. How is 3d not significantly different than 0d when the SE does not overlap? Similarly, how is 7d significantly different than 5d when the SE do overlap? I certainly believe there are some significant differences, but should some bars be delineated as “ab” and “bc” with the overlap of several SE bars? If you provide the post-hoc/Fisher’s LSD test results in a table (possibly a supplemental table), this concern would be addressed immediately.
Lines 178-179: I generally agree, but duration of exposure is important. Perhaps add “when exposed for long enough for damage to occur”.
Line 194: change “dispera” to “dispersal”.
Line 204: change “affects” to “affect”.
Lines 227-228: change to “only indoors” or “was studied under laboratory conditions”.
Lines 236-241: while the plot contains interesting and useful data, the line chart is difficult to read. What about removing the hash lines from the histogram bars? This will likely make the line chart stick out more. And possibly make the line chart a dashed line so it sticks out even more from the histogram lines?
Author Response
Response to Reviewer 1 Comments
Point 1: There are several run-on sentences that make reading the text a little difficult at times. The content is generally good, but should be broken up into more individual sentences at times (see some specific comments below for examples). There are also several spelling and other grammatical errors in the paper. I recommend a thorough read-through from an outside party.（check to make sure you have periods and not commas.
For example, in line 11, there should be a period after “China”, or “More” should not be capitalized.
Line 41: change “fed” to “feed”.
Lines 40-45: break this up into at least two sentences. This is a run on sentence.
Lines 61-62: this is not really a sentence. Are you saying that global warming causes frequent extreme climatic events and abnormal temperature fluctuations in the spring? Please clarify the sentence.
Lines 63-65rewrite as “Because insects are ectotherms, there growth, development and survival are affected by environmental factors, such as temperature, humidity, and precipitation.
Lines 178-179: I generally agree, but duration of exposure is important. Perhaps add “when exposed for long enough for damage to occur”.
Line 194: change “dispera” to “dispersal”.
Line 204: change “affects” to “affect”.
Lines 227-228: change to “only indoors” or “was studied under laboratory conditions
）
Response 1: spelling and rammatical errors had been modified.
Point 2: Lines 63-65: while the statement is not wrong, endotherms are also affected by all these factors. Maybe add something stating why ectotherms are more affected by these factors? Or why they are sensitive to these factors? That they do not internally regulate temperature like endotherms?
Response 2: The explanation of why ectotherms are affected by these factors, why they do not internally regulate temperature like endotherms are added in the paper
Point 3: Line 71: what is meant by “overwintering”. I know what overwintering is generally, and diapause is one way organisms overwinter. This seems a bit vague here.
Response 3: This article would like to quote the way insects cope with adverse environment. Diapause is a way of insect overwintering, so this paper has been modified, citing the literature as insect overwintering, so diapause has been removed.
Point 4: Lines 73-77: this is a run-on sentence. Break up for clarity.
Response 4:It has already been modified.
Point 5: how did you record the temperature of the refrigerator? Some type of thermometer/temperature logger?
Response 5: Stress temperature was measured with a Fuju thermometer. measuring accuracy: ±1°C
Point 6: I have concerns with the statistical analysis when looking at figure 2. How is 3d not significantly different than 0d when the SE does not overlap? Similarly, how is 7d significantly different than 5d when the SE do overlap? I certainly believe there are some significant differences, but should some bars be delineated as “ab” and “bc” with the overlap of several SE bars? If you provide the post-hoc/Fisher’s LSD test results in a table (possibly a supplemental table), this concern would be addressed immediately.
Response 6:Significant labeling error, it has already been modified.
Table 1 ANOVE
|
ANOVE |
|||||
|
Hacthing rate |
|||||
|
|
Sum of Squares |
df |
Mean Square |
F |
Sig. |
|
Between Groups |
1.361 |
5 |
.272 |
22.281 |
.000 |
|
Within Groups |
.293 |
24 |
.012 |
|
|
|
Total |
1.654 |
29 |
|
|
|
Table 2 Multiple Comparisons
|
Multiple Comparisons |
||||||
|
Dependdent Variable: Hacthing rate |
||||||
|
LSD |
||||||
|
(I) Treatment times |
(J) Treatment times |
Mean Difference (I-J) |
SE |
sig |
95%Confidence interval |
|
|
Lower Bound |
Lower Bound |
|||||
|
1d |
3d |
.0684000 |
.0698995 |
.338 |
-.075865 |
.212665 |
|
5d |
.1116200 |
.0698995 |
.123 |
-.032645 |
.255885 |
|
|
7d |
.2622800* |
.0698995 |
.001 |
.118015 |
.406545 |
|
|
10d |
.5862400* |
.0698995 |
.000 |
.441975 |
.730505 |
|
|
CK |
-.0491600 |
.0698995 |
.489 |
-.193425 |
.095105 |
|
|
3d |
1d |
-.0684000 |
.0698995 |
.338 |
-.212665 |
.075865 |
|
5d |
.0432200 |
.0698995 |
.542 |
-.101045 |
.187485 |
|
|
7d |
.1938800* |
.0698995 |
.011 |
.049615 |
.338145 |
|
|
10d |
.5178400* |
.0698995 |
.000 |
.373575 |
.662105 |
|
|
CK |
-.1175600 |
.0698995 |
.106 |
-.261825 |
.026705 |
|
|
5d |
1d |
-.1116200 |
.0698995 |
.123 |
-.255885 |
.032645 |
|
3d |
-.0432200 |
.0698995 |
.542 |
-.187485 |
.101045 |
|
|
7d |
.1506600* |
.0698995 |
.041 |
.006395 |
.294925 |
|
|
10d |
.4746200* |
.0698995 |
.000 |
.330355 |
.618885 |
|
|
CK |
-.1607800* |
.0698995 |
.030 |
-.305045 |
-.016515 |
|
|
7d |
1d |
-.2622800* |
.0698995 |
.001 |
-.406545 |
-.118015 |
|
3d |
-.1938800* |
.0698995 |
.011 |
-.338145 |
-.049615 |
|
|
5d |
-.1506600* |
.0698995 |
.041 |
-.294925 |
-.006395 |
|
|
10d |
.3239600* |
.0698995 |
.000 |
.179695 |
.468225 |
|
|
CK |
-.3114400* |
.0698995 |
.000 |
-.455705 |
-.167175 |
|
|
10d |
1d |
-.5862400* |
.0698995 |
.000 |
-.730505 |
-.441975 |
|
3d |
-.5178400* |
.0698995 |
.000 |
-.662105 |
-.373575 |
|
|
5d |
-.4746200* |
.0698995 |
.000 |
-.618885 |
-.330355 |
|
|
7d |
-.3239600* |
.0698995 |
.000 |
-.468225 |
-.179695 |
|
|
CK |
-.6354000* |
.0698995 |
.000 |
-.779665 |
-.491135 |
|
|
CK |
1d |
.0491600 |
.0698995 |
.489 |
-.095105 |
.193425 |
|
3d |
.1175600 |
.0698995 |
.106 |
-.026705 |
.261825 |
|
|
5d |
.1607800* |
.0698995 |
.030 |
.016515 |
.305045 |
|
|
7d |
.3114400* |
.0698995 |
.000 |
.167175 |
.455705 |
|
|
10d |
.6354000* |
.0698995 |
.000 |
.491135 |
.779665 |
|
|
*. The significance level of the mean difference is 0.05. |
||||||
.
Point 7: Lines 236-241: while the plot contains interesting and useful data, the line chart is difficult to read. What about removing the hash lines from the histogram bars? This will likely make the line chart stick out more. And possibly make the line chart a dashed line so it sticks out even more from the histogram lines?
Response 7: The line chart has made the dashed line.
Reviewer 2 Report
Section 2.3 “The emergence survey of adults Colorado potato beetles” contains a description of the method for accounting for the emergence of adults, but these data are not described in the "Results" section, however are mentioned in the "Discussion". This analysis and graphs should be moved to the Results section. It is also necessary to give a more precise explanation of the scientific novelty of the study, since similar studies have been conducted since the 70s of the last century (possibly even earlier) and a lot of data have been accumulated on this issue.
Author Response
Response to Reviewer 2 Comments
Point 1: Section 2.3 “The emergence survey of adults Colorado potato beetles” contains a description of the method for accounting for the emergence of adults, but these data are not described in the "Results" section, however are mentioned in the "Discussion". This analysis and graphs should be moved to the Results section. It is also necessary to give a more precise explanation of the scientific novelty of the study, since similar studies have been conducted since the 70s of the last century (possibly even earlier) and a lot of data have been accumulated on this issue.
Response 1: The emergence survey of adults Colorado potato beetles data have been put into the results section and analyzed
Round 2
Reviewer 1 Report
General
While the paper is much improved, there are still some minor typos and grammatical errors in the manuscript. It appears that many of the brackets for the citations are lacking spaces from the preceding words. I point out some below, but the paper could use another read through. The content is good, so possibly a person not associated with the paper could help?
Specific
Lines 11-12: I believe the authors have placed commas where periods should be.
Line 44: delete space after period and add a space after the period (before “As Colorado”).
Lines 61-62: I do not follow this general statement. It reads as a random fact. Possibly expand or link to the proceeding sentence?
Line 67: while not wrong, this statement could be a little more specific as there are several thermoregulatory mechanisms (e.g., behavioral, physiological, and morphological). So maybe be more specific to ectotherms and just say “behavioral thermoregulation” as they are physiologically limited.
Line 95: remove citation #33 superscript.
Line 188: looking and figure 3 and the figure legend, it is unclear if this is the first date of beetle emergence. Please clarify in the legend.
Lines 251-257: the mean temperatures are interesting, but what about adding in error bars in the form of standard deviations to show the levels of variance?
Lines 257-258: is it necessary to include every year on the x-axis? This makes the axis very busy.
Author Response
Response to Reviewer 1 Comments
Point 1: Lines 11-12: I believe the authors have placed commas where periods should be.
Line 44: delete space after period and add a space after the period (before “As Colorado”).
Line 95: remove citation #33 superscript.
Response 1: They have already been modified.
Point 2: Lines 61-62: I do not follow this general statement. It reads as a random fact. Possibly expand or link to the proceeding sentence?
Response 2: It has already been modified.
Point 3: Line 67 while not wrong, this statement could be a little more specific as there are several thermoregulatory mechanisms (e.g., behavioral, physiological, and morphological). So maybe be more specific to ectotherms and just say “behavioral thermoregulation” as they are physiologically limited.
Response 3: This statement have been changed to a more specific statement.
Point 4: Line 188: looking and figure 3 and the figure legend, it is unclear if this is the first date of beetle emergence. Please clarify in the legend.
Response 4:It has already been clarified in the legend..
Point 5: Lines 251-257: the mean temperatures are interesting, but what about adding in error bars in the form of standard deviations to show the levels of variance?
Response 5: It has already been modified.
Point 6: Lines 257-258: is it necessary to include every year on the x-axis? This makes the axis very busy.
Response 6: It has already been modified.
