Identification of 35 C-Type Lectins in the Oriental Armyworm, Mythimna separata (Walker)
School of Plant Protection, Anhui Agricultural University, Hefei 230036, China
Anhui Province Key Laboratory of Integrated Pest Management on Crops, Anhui Agricultural University, Hefei 230036, China
Author to whom correspondence should be addressed.
Academic Editor: Peter Piermarini
Received: 28 May 2021 / Revised: 12 June 2021 / Accepted: 14 June 2021 / Published: 16 June 2021
The oriental armyworm Mythimna separata is a lepidopteral agricultural pest that causes serious damage to many crops, such as maize, wheat, and sorghum. To control this pest, it is advisable to take comprehensive measures, including the use of chemical pesticides, microbial pesticides, and cultural practices. However, microbial pesticides (entomopathogens) can be eliminated by the insect immune system. C-type lectins (CTLs) are a family of pattern-recognition receptors that recognize carbohydrates and mediate immune responses. C-type lectins in the oriental armyworm have not yet been identified and characterized. In this study, a transcriptome of M. separata larvae was constructed and a total of 35 CTLs containing single or dual carbohydrate-recognition domains (CRDs) were identified from unigenes. Phylogenetic analyses, sequence alignments and structural predictions were performed. Gene expression profiles in different developmental stages, naïve larval tissues, and bacteria/fungi-challenged larvae were analyzed. Overall, our findings indicate that most dual-CRD CTLs are expressed in mid-late-stage larvae, pupae, and adults. Bacterial and fungal challenges can stimulate the expression of many CTLs in larval hemocytes, fat body, and midgut. Our data suggest the importance of CTLs in immune responses of M. separata.