The Impact of Ultraviolet-B Radiation on the Sugar Contents and Protective Enzymes in Acyrthosiphon pisum
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Insect Culture
2.2. Mode of UV-B Treatment
2.3. Determination of Trehalose and Glycogen Content
2.4. Preparation of Enzyme Solution
2.5. Protein Content Determination
2.6. Protective Enzyme Activities of Pea Aphids
2.7. Statistical Analysis
3. Results
3.1. Interaction between UV-B Treatment and Generation
3.2. Effects of UV-B Treatments on the Trehalose Content of Aphids
3.3. Effects of UV-B Treatments on the Glycogen Content of Aphids
3.4. Effects of UV-B Treatments on the Protein Content of Aphids
3.5. Effects of UV-B Treatment on SOD Activity of Aphids
3.6. Effects of UV-B Treatment on the POD Activity of Aphids
3.7. Effects of UV-B Treatment on the CAT Activity of Aphids
3.8. Comparison of UV-B Treatments on Sugar and the Enzyme Activity at G0 and G7 Generations
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Measurement | Morph | Type III Sum of Squares | Df | Mean Square | F-Value | p-Value |
---|---|---|---|---|---|---|
Trehalose | Red | 1.61 | 28 | 0.06 | 2.85 | <0.001 |
Green | 0.80 | 28 | 0.03 | 1.48 | 0.089 | |
Glycogen | Red | 4.62 | 28 | 0.17 | 3.89 | <0.001 |
Green | 2.66 | 28 | 0.10 | 4.88 | <0.001 | |
Protein | Red | 489.78 | 28 | 17.49 | 12.09 | <0.001 |
Green | 352.13 | 28 | 12.58 | 12.91 | <0.001 | |
SOD | Red | 701.47 | 28 | 25.05 | 54.56 | <0.001 |
Green | 300.34 | 28 | 10.73 | 31.71 | <0.001 | |
POD | Red | 3.28 | 28 | 0.12 | 0.54 | 0.966 |
Green | 20.08 | 28 | 0.72 | 1.78 | 0.024 | |
CAT | Red | 36.92 | 28 | 1.32 | 4.90 | <0.001 |
Green | 27.46 | 28 | 0.98 | 2.88 | <0.001 |
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Li, C.; Yuan, W.; Gou, Y.; Zhang, K.; Zhang, Q.; Zhou, J.-J.; Liu, C. The Impact of Ultraviolet-B Radiation on the Sugar Contents and Protective Enzymes in Acyrthosiphon pisum. Insects 2021, 12, 1053. https://doi.org/10.3390/insects12121053
Li C, Yuan W, Gou Y, Zhang K, Zhang Q, Zhou J-J, Liu C. The Impact of Ultraviolet-B Radiation on the Sugar Contents and Protective Enzymes in Acyrthosiphon pisum. Insects. 2021; 12(12):1053. https://doi.org/10.3390/insects12121053
Chicago/Turabian StyleLi, Chunchun, Weining Yuan, Yuping Gou, Kexin Zhang, Qiangyan Zhang, Jing-Jiang Zhou, and Changzhong Liu. 2021. "The Impact of Ultraviolet-B Radiation on the Sugar Contents and Protective Enzymes in Acyrthosiphon pisum" Insects 12, no. 12: 1053. https://doi.org/10.3390/insects12121053