Next Article in Journal
ResNet-Locust-BN Network-Based Automatic Identification of East Asian Migratory Locust Species and Instars from RGB Images
Previous Article in Journal
Process of Attack on Cashew Tree Branches by Diastocera trifasciata (Coleoptera: Cerambycidae) and the Relationship between These Attacks and the Phenological Stages in the Gbêkê Region (Central Côte d’Ivoire)
Open AccessArticle

EPNs Exhibit Repulsion to Prenol in Pluronic Gel Assays

by Tiffany Baiocchi 1,†, Chunjie Li 2,† and Adler R. Dillman 1,*
1
Department of Nematology, University of California, Riverside, CA 92521, USA
2
Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Insects 2020, 11(8), 457; https://doi.org/10.3390/insects11080457
Received: 16 June 2020 / Revised: 13 July 2020 / Accepted: 15 July 2020 / Published: 22 July 2020
(This article belongs to the Special Issue Entomopathogenic Nematodes: Lethal Parasites of Insects)
Entomopathogenic nematodes (EPNs) are lethal parasites of insects that have become valuable in biological control and as a model system for studying host–parasite interactions, behavioral ecology, neurobiology, and genomics, among other fields. Their ability to locate hosts is paramount to successful infection and host seeking has been extensively studied in many species in the lab. Here, we explored the usefulness of pluronic gel as a medium to assess EPN host seeking in the lab by characterizing the response of Steinernema carpocapsae, S. feltiae, S. glaseri, S. riobrave, Heterorhabditis bacteriophora, and H. indica to the odor prenol. We found that the infective juveniles (IJs) of these species were repelled by prenol in pluronic gel. We then evaluated how storing the IJs of S. carpocapsae, S. feltiae, and S. glaseri for different amounts of time affected their behavioral responses to prenol. The response of S. carpocapsae was significantly affected by the storage time, while the responses of S. feltiae and S. glaseri were unaffected. Our data support the notion that pluronic gel is a useful medium for studying EPN behavior and that the response of S. carpocapsae to informative odors is significantly affected by long-term storage. View Full-Text
Keywords: entomopathogenic nematodes; PF127; pluronic gel; chemotaxis entomopathogenic nematodes; PF127; pluronic gel; chemotaxis
Show Figures

Figure 1

MDPI and ACS Style

Baiocchi, T.; Li, C.; Dillman, A.R. EPNs Exhibit Repulsion to Prenol in Pluronic Gel Assays. Insects 2020, 11, 457.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop