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10.3390/insects11060392
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Article

Are the Yellow and Red Marked Club-Tail Losaria coon the Same Species?

by
Zhen-Bang Xu
1,†,
Yun-Yu Wang
2,†,
Fabien L. Condamine
3,
Adam M. Cotton
4 and
Shao-Ji Hu
5,6,*
1
School of Agriculture, Yunnan University, Kunming 650500, China
2
Plant Protection College, Yunnan Agricultural University, Kunming 650201, China
3
CNRS, Institut des Sciences de l’Evolution de Montpellier (Université de Montpellier|CNRS|IRD|EPHE), Place Eugène Bataillon, 34095 Montpellier, France
4
86/2 Moo 5, Tambon Nong Kwai, Hang Dong, Chiang Mai 50230, Thailand
5
Yunnan Key Laboratory of International Rivers and Transboundary Eco-security, Yunnan University, Kunming 650500, China
6
Institute of International Rivers and Eco-Security, Yunnan University, Kunming 650500, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally.
Insects 2020, 11(6), 392; https://doi.org/10.3390/insects11060392
Received: 15 May 2020 / Revised: 19 June 2020 / Accepted: 23 June 2020 / Published: 24 June 2020
(This article belongs to the Collection Butterfly Diversity and Conservation)
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Abstract

Losaria coon (Fabricius, 1793) is currently comprised of ten subspecies, which were originally described under two names, Papilio coon and P. doubledayi before 1909, when they were combined as one species. The main difference between them is the colour of abdomen and hindwing subterminal spots—yellow in coon and red in doubledayi. Wing morphology, male and female genitalia, and molecular evidence (DNA barcodes) were analysed for multiple subspecies of L. coon and three other Losaria species—rhodifer, neptunus, and palu. Our molecular data support the separation of L. coon and L. doubledayi stat. rev. as two distinct species, with L. rhodifer positioned between them in phylogenetic analyses. Wing morphology and genitalic structures also confirm the molecular conclusions. Our findings divide L. coon into two species occupying different geographic ranges: with L. coon restricted to southern Sumatra, Java, and Bawean Island, while L. doubledayi occurs widely in regions from North India to northern Sumatra, including Hainan and Nicobar Islands. Hence, future conservation efforts must reassess the status and threat factors of the two species to form updated strategies.
Keywords: species delimitation; distribution range; molecular phylogeny; wing pattern; genitalic structure species delimitation; distribution range; molecular phylogeny; wing pattern; genitalic structure

1. Introduction

Losaria coon (Fabricius, 1793) is a club-tail papilionid butterfly of the genus Losaria (Moore, 1902), which contains three other species, namely L. rhodifer (Butler, 1876), L. neptunus (Guérin-Méneville, 1840), and L. palu (Martin, 1912) [1]. L. coon is distributed across the Oriental tropics, ranging from northern India to Java, including Indochina, southern China, and Sumatra [2].
The species currently recognised as Losaria coon contains a number of taxa, some of which were originally described as species or as subspecies of the yellow or red marked species, coon and doubledayi, respectively, which were originally described in the genus Papilio (Linnaeus, 1758) [3,4]. All 19th century publications (e.g., Rothschild [5]) treated P. coon and P. doubledayi as separate species, as did Moore [6]. Jordan [7], without explanation united them as a single species, Papilio coon, which has been followed by all subsequent publications (e.g., Evans [8] and Talbot [9]). Modern works have placed the species in other genera than Papilio, mostly treating it in the genus Atrophaneura (Reakirt, 1865), following Ford [10]. Tsukada and Nishiyama [2] and Hancock [1] separated the club-tailed species in Losaria (Moore, 1902), where they are currently placed. This genus designation is also supported by the current molecular phylogenies of the family Papilionidae [11].
To date, ten subspecies have been placed under L. coon [2,12,13,14], which can be divided into two groups with the following morphological characters and geographic distributions, as shown in Figure 1: (a) four subspecies occupying western and northern Java, southwest Sumatra, and Bawean Island, respectively, ssp. palembanganus (Rothschild, 1895), ssp. coon, ssp. patianus (Fruhstorfer, 1898), and ssp. sangkapurae (Bollino and Sala, 1992), possessing yellow marginal spots on the hindwing (referred to as yellow marked coon) with the hindwing discal cell patch reaching the base of the cell; (b) six subspecies found from northeastern India, northern Myanmar, Nicobar Islands, Indochina and Malay Peninsula, Hainan Island, and northern Sumatra, namely ssp. cacharensis (Butler, 1885), ssp. putaoa (Tytler, 1939), ssp. sambilanga (Doherty, 1886), ssp. doubledayi (Wallace, 1865), ssp. insperatus (Joicey and Talbot, 1921), and ssp. delianus (Fruhstorfer, 1895), which all share red marginal spots on the hindwing (referred to as red marked coon), and the hindwing discal cell patch does not reach the base of the cell.
The yellow and red marked coon are separated from each other geographically, even in Sumatra, where the yellow marked ssp. palembanganus and the red marked ssp. delianus are not sympatric, according to available records, as shown in Figure 1. The constant morphological differences coupled with this distribution pattern leave an unanswered question—whether the yellow and red marked coon really belong to the same species.
Speciation with limited morphological differentiation among closely related taxa is not unique to L. coon in Lepidoptera. Some recent studies revealed that speciation caused by recent vicariance produced multiple morphologically similar taxa which have long been treated as subspecies of the same species (e.g., the dragontail butterflies of genus Lamproptera (Gray, 1832) [15], the swordtail butterflies of subgenus Pazala (Moore, 1888) [16,17,18], the Asian moon moths of genus Actias (Leach, 1815) [19], the hawkmoths of genus Cechetra (Zolotuhin and Ryabov, 2012) [20], and genus Laothoe (Fabricius, 1807) [21]). Apart from traditional morphological comparisons, such as wing pattern and genitalia analyses, the abovementioned studies applied mitochondrial DNA analyses (mostly barcode sequences) [22] to facilitate elucidating some long-lasting taxonomic confusions.
The aim of the present study is to revise the taxonomic identities of the yellow and red marked coon using mitochondrial DNA barcode sequences combined with morphological comparisons. Our findings will first clarify taxonomic confusions, draw updated geographic ranges for relevant taxa, and finally benefit from the formulation of better conservation strategies [12].

2. Materials and Methods

2.1. Taxon Sampling

Specimens of L. coon (including four subspecies), L. rhodifer (Butler, 1876), L. neptunus (Guérin-Méneville, 1840) (including two subspecies), and L. palu (Martin, 1912) in this study were mainly sampled from the authors’ private collections. Subspecies identification for Losaria species mainly followed Tsukada and Nishiyama [2]. Specimens were collected and dried in paper triangles at room temperature until use. For each individual used in molecular analysis, two legs from the same side were taken for DNA extraction before the specimen was rehydrated for spreading. An individual of Pharmacophagus antenor (Drury, 1773) and an individual of Pachliopta polyphontes (Boisduval, 1836) were chosen as outgroups for phylogenetic analyses, similar to those used by Hancock [1]. In addition, three sequences, including two individuals of L. coon doubledayi and an individual of L. neptunus neptunus, were mined from the Barcode of Life Database v.4 (BOLD) (http://www.boldsystems.org) for the molecular analyses. The collected data and GenBank accession numbers of all samples and mined sequences used in our molecular analyses are listed in Table 1.
Due to difficulties obtaining fresh samples of some Indian, Myanmar, and Indonesian insular subspecies, namely L. coon cacharensis (Butler, 1885), L. coon sambilanga (Doherty, 1886), L. coon putaoa (Tytler, 1939), L. coon delianus (Fruhstorfer, 1895), L. coon patianus (Fruhstorfer, 1898), and L. coon palembanganus (Rothschild, 1896), these taxa are not included in the molecular analysis of this study, but will be discussed based on morphological characters from photos of type specimens and original descriptions (Appendix A).

2.2. DNA Extraction and Amplification

The phenol–chloroform protocol was used to extract genomic DNA from two legs pulled from the same side of a specimen of all sampled taxa. The legs were homogenised in protease buffer containing 450 μL STE (10mmol/L Tris-HCl, 1 mmol/L EDTA, 100 mmol/L NaCl, pH = 8.0), 25 μL Proteinase K (20 mg/mL) and 75 μL SDS (10%) and incubated at 55 °C for 12 h to rehydrate and lyse the tissue. The subsequent extraction protocol followed that reported by Hu et al. [23]. The resultant genomic DNA was preserved at −40 °C.
The polymerase chain reaction (PCR) was carried out in a 25 μL system by using the TaKaRa Ex Taq Kit (TaKaRa Biotechnology Co., Ltd., Dalian, China). The system contained 2.5 μL 10× PCR buffer, 2.0 μL MgCl2 (25 mmol/L), 2.0 μL dNTP mixture (2.5 mmol/L each), 0.25 μL Taq DNA polymerase (5 U/μL), and 0.5 μL each of forward and reverse primers (20 μmol/L). The mitochondrial cox1 gene fragment (the DNA barcode) was amplified and sequenced with the following primers LCO1490 (5′- GGT CAA CAA ATC ATA AAG ATA TTG G-3′) and HCO2198 (5′- TAA ACT TCA GGG TGA CCA AAA AAT CA-3′) [24]. The thermal profile of PCR consisted of an initial denaturation at 95 °C for 3 min, 30 cycles of denaturation at 94 °C for 1 min, annealing at 50 °C for 1 min, and elongation at 72 °C for 1 min; then a final elongation at 72 °C for 5 min. The sequences were obtained using an ABI Prism 3730 sequencer (Applied Biosystems, Foster City, CA, USA).

2.3. Phylogenetic Analyses

We proofread and aligned the raw sequences with Clustal W [25] in BioEdit 7.0.9 [26] by examining the chromatograms to ascertain polymorphic sites, and problematic sequences were excluded when double peaks were present in the chromatograms. MEGABLAST was applied to check the identities of all sequences against the genomic references and nucleotide collections in GenBank, and amino acid translation was realised with the invertebrate mitochondrial criterion in MEGA 7.0 [27] to detect possible Numts (nuclear copies of mtDNA fragments). A search for non-synonymous mutations in-frame stop codons and indels was carried out to further minimise the existence of cryptic Numts [28,29]. The Kimura two-parameter distances [30] between taxa were calculated in MEGA 7.0.
All sequences were used in phylogenetic reconstructions without pruning identical haplotypes, since the monophyly of samples identified as species by morphological characters [2,13,14] needs to be tested. The phylogeny was reconstructed using a Bayesian Inference (BI) as implemented in MrBayes 3.2.6 [31], with the most appropriate partition scheme recovered by PartitionFinder 2.1.1 [32] using the unlinked branch lengths and the greedy algorithm. We used the partitioning scheme among site rate variation, suggested by PartitionFinder, but instead of selecting one substitution model a priori, we used reversible-jump Markov Chain Monte Carlo (rj-MCMC) to allow sampling across the entire substitution rate model space [33]. BI analyses consisted of two independent runs, each with eight rj-MCMC running for five million generations (sampled every 1000th generation) to calculate the clade posterior probabilities (PP). The marginal likelihood estimate was performed with the stepping-stone sampling [34], implemented in MrBayes with 100 steps, each with 10 million generations, and a diagnostic frequency of 1000. Through the computation of Bayes factors, marginal likelihood estimates were used to compare the model fit of an unconstrained topology with the fit of a constrained topology, in which a group of specimens is forced to be monophyletic.

2.4. Molecular Species Delimitation

In order to test the delimitation of Losaria species, we relied on a tree-based approach with the Bayesian–Poisson tree process model (bPTP; https://species.h-its.org/ptp/) [35], and on a DNA-based approach with the Automatic Barcode Gap Discovery (ABGD; https://bioinfo.mnhn.fr/abi/public/abgd) [36]. The bPTP model estimates the probability of each clade being a putative species based on the branch lengths of a tree. The bPTP analyses were performed with the guide tree, reconstructed from the BI in MrBayes with the following parameters: 100,000 MCMC generations, thinning every 100 generations, 0.1% of generation discarded as burn-in. In comparison, the ABGD model estimates the barcode gap separated the intraspecific from the interspecific molecular divergence. Thus, the prior maximum divergence of intraspecific diversity (P) is an important component of ABGD as it provides approximate indications on the barcode gap. If P is set too high, the whole dataset will be considered as a single species, while if P is set too low, only identical sequences will be considered as part of the same species. Previous results showed that the number of species ranges from 1 (generally when P = 0.1) to a large number of species that corresponds to groups of identical sequences (generally when P = 0.001). We followed this practice and set the range of P from 0.001 to 0.1 to explore a lumped versus split delimitation. In addition, the Kimura two-parameter distance was selected but the analyses made with the Jukes–Cantor model provided identical results. The sensitivity of the method to gap width was left by default (1.5), but analyses performed with higher gap width (2 and 3) yielded identical results. The remaining parameters were left by default. Finally, the Monophylizer (http://monophylizer.naturalis.nl/) [37] was performed on the obtained BI tree to test the monophyly of each identified taxon.

2.5. Morphological Comparison

Specimens were spread for examination, with the anal scent scales exposed. Species identification was performed prior to molecular work. All spread specimens were photographed using a digital camera, and Adobe Photoshop CS (Adobe, San Jose, CA, USA) was used to adjust the exposure of these photos.
The methods for preparing male and female genitalia followed Hu, Cotton, Condamine, Duan, Wang, Hsu, Zhang, and Cao [16]. The abdomen was removed from the specimen and placed into a 1.5 mL microcentrifuge tube, and 1 mL water was added to rehydrate the tissue at 50 °C for 30 min, then 1 mL 10% sodium hydroxide solution was used to digest soft tissue at 70 °C for 1h. The treated abdomen was neutralised with 2% acetic acid and then dissected in a water-filled Petri dish under the stereoscope to remove residual tissues, scales, and hair. The genitalia were then transferred to 80% glycerol for 12 h to render them transparent. Photographs were taken with a Nikon DMX1200 digital camera (Nikon, Japan), mounted on a Nikon SMZ1500 stereoscope (Nikon, Japan) and automatically stacked using Helicon Focus 7 (Helicon Software, Kharkiv, Ukraine). All parts of the genitalia were fixed on a glue card with water-soluble white glue and pinned with the specimen after observation and photography.

3. Results

3.1. Molecular Phylogenetic Relationships

Bayesian phylogenetic analyses converged well, as indicated by the average standard deviation of split frequencies close to 0 (0.003864), potential scale reduction factors equal to 1 (maximum = 1.003), and effective sample size >> 200 for all parameters. The phylogeny of the Losaria species was recovered as two major clades with maximal PP value, as shown in Figure 2. The clade (PP = 1) containing both the yellow and red marked coon and L. rhodifer is younger than the other clade including L. neptunus and L. palu (PP = 1).
In the first major clade, the yellow and red marked coon is divided into two monophyletic subclades by L. rhodifer (PP = 0.83), indicating a specific level divergence between the two morphological types. The subclade of yellow marked coon contains L. coon coon and L. coon sangkapurae, and that of red marked coon contains L. coon insperatus and L. coon doubledayi, which were further split into two paraphyletic branches, as shown in Figure 2. The basal branch includes samples collected from the northern Malay Peninsula (not far from the type locality of doubledayi), and the sister branch includes samples from Indochina, implying a certain degree of divergence between the two geographical populations, as shown in Figure 2.
The estimations of marginal likelihoods with stepping-stone sampling in MrBayes confirmed the non-monophyly of Losaria coon when all specimens (coon coon, coon sangkapurae, coon doubledayi, and coon insperatus) are constrained to form a monophyletic group (logL = −1955.68 for the unconstrained analysis, logL = −1960.01 for the constrained analysis and Bayes factors = 8.66).

3.2. Molecular Species Delimitation

Both bPTP and ABGD approaches produced similar results of taxon delimitation. They identified five Losaria species and four subspecific taxa from the Bayesian tree, excluding two outgroups. The bPTP model identified the yellow and red marked coon as separate species with a probability of 0.81 for L. coon doubledayi and of 0.89 for L. coon coon, and with probabilities above 0.65 for L. rhodifer (probability = 0.98) L. neptunus (probability = 0.76) and L. palu (probability = 0.87), as shown in Figure 2. For subspecies, the bPTP model identified subspecies of the red marked coon with supporting values ranging from 0.66 to 0.81, whereas a combination of L. coon sangkapurae with L. coon coon produced a very low supporting value of 0.03, as shown in Figure 2. The ABGD approach also identified the yellow and red marked L. coon as distinct species in all the analyses, whatever the settings for the nucleotide model, the gap width, and the range of P. At a prior intraspecific divergence P > 0.008, we inferred five groups of sequences corresponding to the five Losaria species. Four subspecific taxa are recovered at P > 0.002. ABGD lumps all coon coon, coon sangkapurae, coon doubledayi, coon insperatus, and rhodifer specimens into a single species for a prior intraspecific divergence P > 0.02.
The monophylizer analysis also identified the yellow and red marked coon as two monophyletic clades in the BI tree, along with L. rhodifer, L. neptunus, and L. palu. Paraphyletic clades were found in subspecies, such as L. coon coon (one of the yellow marked coon) and the two doubledayi races (red marked coon) from southern Thailand to the Malay Peninsula and Indochina, as shown in Table 2.
The Kimura two-parameter (K2P) distances between the yellow and red marked coon reached 2.95%. The K2P distances between all taxa within both groups, and the species of L. rhodifer, L. neptunus, and L. palu ranged from 0.19% to 9.61%, with that between L. coon coon and L. coon sangkapurae being the smallest, and that between L. rhodifer and L. palu being the greatest, as shown in Table 3. For those distances between the yellow and red marked coon, the overall range fell from 2.44% to 3.17%, which exceeds the proposed barcoding gap of 2% [38], while the K2P distances within the yellow or red marked coon, respectively, remained at infraspecific level (0.19–1.08%), as shown in Table 3. It is noteworthy that the K2P distances between the yellow marked coon and L. coon insperatus (one of the red marked subspecies) are greater (3.57–3.77%) than those between the yellow marked coon and L. rhodifer (3.17–3.35%), and the distances between the yellow marked coon and the Malay Peninsular doubledayi are very close to those between the yellow marked coon and L. rhodifer (3.08–3.28% vs. 3.17–3.35%), as shown in Table 3.
Given the aforementioned molecular evidence, we propose that the yellow and red marked coon belong to two distinct species, namely Losaria coon (Fabricius, 1793) and Losaria doubledayi (Wallace, 1865) stat. rev., respectively. The subsequent morphological analyses and discussion will be based on the separated names.

3.3. Morphological Differences

Comparison of the wing morphology between L. coon and L. doubledayi showed constant differences, as illustrated in Figure 3. Beside the evident yellow and red colouration on the abdomens and hindwings of both species, the following characters are also important in separating the two species: (1) the hindwing white discal cell patch reaches the wing base in L. coon, but only reaches about half way to the base in L. doubledayi (a); (2) the forewing ground colour in L. coon is buffish brown, whereas in L. doubledayi it is blackish (b); the colour of the male scent scales is light buffish in L. coon, but greyish black in L. doubledayi (f); (3) the subterminal spot in the cell M2 of the hindwing is usually larger in L. coon than in L. doubledayi (c); (4) the junction between the termen and the lobe at the end of vein CuA1 on the hindwing is evidently angled in L. coon, but much straighter in L. doubledayi (d); (5) in the hindwing tail, the stalk is broader in L. coon than that in L. doubledayi, and the club is narrower in L. coon than that in L. doubledayi (e).
In overall appearance, among the remaining three Losaria species, L. rhodifer is more similar to L. coon and L. doubledayi. However, the more fragmented hindwing white patch, much larger subterminal crimson spots, and the iconic crimson-tipped tail are ready separation characters, as shown in Figure 4. Morphological similarity and differences of L. rhodifer agrees with its phylogenetic position obtained by our molecular analyses, as shown in Figure 2. The other two species, L. neptunus and L. palu, are less closely related to the previously mentioned taxa, judging from the distinctive yellow-tipped abdomens and lack of hindwing discal patches, as shown in Figure 4.
Male genitalia of available subspecies were dissected, constant differences were found between L. coon and L. doubledayi, while the characters remain consistent within each group, as shown in Figure 5. The main differences in male genitalia are: (1) the dorsal view of the superuncus is broader in L. coon (0.29 ± 0.03 mm, n = 12), while it is rather slender (0.16 ± 0.02 mm, n = 15) in L. doubledayi; (2) the tip of the valve is only truncated in L. coon but strongly indented in L. doubledayi, forming a bifid tip; (3) the apical half of the juxta is 1.3 to 1.5 times broader in L. coon than in L. doubledayi.
Female genitalia of available subspecies were dissected, constant differences were found between L. coon and L. doubledayi, while the characters remain consistent within each group, as shown in Figure 6. The main differences in female genitalia are: (1) the ostium is smaller in L. coon but broader in L. doubledayi; (2) the lamella antevaginalis sunk inwardly, forming a crescent edge, in L. coon, while forming an even edge in L. doubledayi; (3) the lamella postvaginalis is 0.5 to 0.7 times shorter and 1.8 to 2.0 times broader in L. coon than that in L. doubledayi; (4) the lateral area surrounding the ostium is less developed in L. coon than in L. doubledayi; (5) the signum is leaf-shaped with one end longer than the other in L. coon, while nearly round in L. doubledayi, and the length of the signum in L. coon is 1.7 to 2.0 times longer than that in L. doubledayi.
Among the male and female genitalia of the other three species of Losaria, those of L. rhodifer are similar to the previously mentioned two species, but the shape of valve tip of the male genitalia and the ostium, lamella antevaginalis, and signum are still different, as shown in Figure 7A and Figure 8A,D. Such resemblance indicates that L. rhodifer is closely related to both L. coon and L. doubledayi, as previously mentioned. The genitalia of both sexes of L. neptunus and L. palu are all significantly different, as shown in Figure 7B,C and Figure 8B–F, supporting the phylogenetic relationships with L. coon and L. rhodifer, as inferred by the molecular data.

3.4. Updated Subspecies Checklist of Losaria (Moore, 1902)

Losaria (Moore, 1902)
Losaria (Moore, 1902); Lepidoptera Indica, 5(57): 184; TS: Papilio coon (Fabricius, 1793).
  Balignina (Moore, 1902); Lepidoptera Indica, 5(57): 187; TS: Papilio neptunus (Guérin-Méneville, 1840).
Losaria palu (Martin, 1912)
Papilio palu (Martin, 1912); D. ent. Z. Iris, 26(3): 164; TL: “Lewara ... 2000 m ... Palu” [Lewara, Palu, Sulawesi, Indonesia].
  Distribution: Only known from the vicinity of Palu, Sulawesi.
Losaria neptunus (Guérin-Méneville, 1840)
  L. neptunus manasukkiti (Cotton, Racheli and Sukhumalind, 2005)
  Losaria neptunus manasukkiti (Cotton, Racheli and Sukhumalind, 2005); Fragm. ent., 37(1): 130, f. 1-2; TL “Kamphuan, Ranong, Thailand”.
  Distribution: Ranong Province, Thailand and southernmost Myanmar.
  L. neptunus neptunus (Guérin-Méneville, 1840)
  Papilio neptunus (Guérin-Méneville, 1840); Revue zool., 3(2): 43; TL: “Côte Malaye” [Malay Peninsula].
     Papilio thetys (Guenée, 1872); Mém. Soc. Phys. Hist. nat. Genève 21(2): 378 pl. f. 5; TL: “pas d’indication de localité” [without indication of locality].
  Distribution: West Malaysia, southernmost Thailand.
  L. neptunus creber (van Eecke, 1914)
  Papilio neptunus ssp. creber (van Eecke, 1914); Notes Leyden Mus., 36(3/4): 197; TL: “Sinabang; Sibigo” [Sinabang and Sibigo, Simeulue Is., Indonesia].
  Distribution: Simeulue Is. [Indonesia].
  L. neptunus fehri (Honrath, 1892)
  Papilio neptunus var. fehri (Honrath, 1892); Berl. Ent. Z., 36(2): 432; TL: “Insel Nias”.
  Distribution: Nias Is. [Indonesia].
  L. neptunus lepida (Hanafusa, 1994)
  Losaria neptunus lepida (Hanafusa, 1994); Futao, 17: 18, pl. 3, f. 13-14; TL: “Tanahmasa Is., Batu Islands, Indonesia”.
  Distribution: Tanahmasa Is. [Indonesia].
  L. neptunus eminens (Hanafusa, 1990)
  Losaria neptunus eminens (Hanafusa, 1990); Futao, 6: 9, pl. 2, f. 5-7; TL: “Sipora Island, Kep. Mentawai, Indonesia”.
  Distribution: Sipora Is. [Indonesia].
  L. neptunus siborangitana (Tsukada and Nishiyama, 1980)
  Losaria neptunus ssp. siborangitana (Tsukada and Nishiyama, 1980); in Tsukada (Ed.), Butts SE Asian Islands, 1: 259, pl. 52, f. 3-4; TL: “near Bandar Baru N. Sumatra”. [near Bandar Baru, Northern Sumatra, Indonesia].
    Papilio neptunus var. Sumatrana (Hagen, 1894); Dt. ent. Z. Iris, 7(1): 21; TL: “Sumatra ... Vorbergen Delis” [Medan foothills, North Sumatra, Indonesia]. [Junior homonym of Papilio cloanthus var. Sumatrana (Hagen, 1894), chosen by Moonen [39] as the senior name of three homonyms introduced in the same work.]
     Pachliopta neptunus muadder Koçak and Kemal, 2000; Misc. Pap. Centre ent. Stud., 71: 3 [unnecessary replacement name].
  Distribution: Northern Sumatra [Indonesia].
  L. neptunus padanganus (Rothschild, 1908)
  Papilio neptunus padanganus (Rothschild, 1908); Novit. zool., 15(1): 165; TL: “West Sumatra: Padang and Padang Sidempoean”. [Padang and Padang Sidempuan, Western Sumatra, Indonesia].
  Distribution: Western Sumatra [Indonesia].
  L. neptunus doris (Rothschild, 1908)
  Papilio neptunus doris (Rothschild, 1908); Novit. zool., 15(1): 165; TL: “North Borneo”.
  Distribution: The island of Borneo.
  L. neptunus dacasini (Schröder, 1976)
  Pachliopta neptunus ssp. dacasini (Schröder, 1976); Ent. Z., 86(24): 269; TL: “Philippinen, Palawan”.
  Distribution: Palawan Is. [Philippines].
  L. neptunus matbai (Schröder and Treadaway, 1990)
  Losaria neptunus ssp. matbai (Schröder and Treadaway), 1990; Ent. Z., 100: 381; TL: “Philippinen, Sulu-Archipel, Tawitawi-Gruppe” [Tawitawi Islands, Sulu Archipelago, Philippines].
 Distribution: Tawitawi Is. [Sulu Archipelago, southern Philippines].
Losaria coon (Fabricius, 1793)
  L. coon palembanganus (Rothschild, 1896)
  Papilio coon palembanganus (Rothschild, 1896); Novit. zool., 3(4): 421; TL: “Upper Musi River, Palembang district, Sumatra; 103° E. Long., 3° S. Lat.”.
  Distribution: Southern Sumatra [Indonesia].
  L. coon coon (Fabricius, 1793)
  Papilio Coon (Fabricius, 1793); Ent. syst., 3(1): 10, no. 27; TL: “China” [loc. err. = Java].
     Papilio Hypenor (Godart, 1819); in: Latreille M. and Godart M., Encyc. méth., 9(1): 65; TL: “Java”.
  Distribution: West Java [Indonesia].
  L. coon patianus (Fruhstorfer, 1898)
  Papilio coon patianus (Fruhstorfer, 1898); Soc. ent., 12(23): 179; TL: “Pati in der Residenz Djapara, dem nördlichsten Teile von Central-Java” [Pati, northernmost part of Central Java].
  Distribution: Northern Central Java [Indonesia].
L. coon sangkapurae (Bollino and Sala, 1992)
  Atrophaneura coon sangkapurae (Bollino and Sala, 1992); Trop. Lepid., 3(2): 119, f. 1, 5-8; TL: “INDONESIA: Pulau Bawean” [Bawean Is., Indonesia].
  Distribution: Bawean Is. [Indonesia].
Losaria rhodifer (Butler, 1876)
Papilio rhodifer (Butler, 1876); Ent. month. Mag. 13(147): 57; TL: “Andaman Islands”.
  Distribution: Andaman Islands, [India].
Losaria doubledayi (Wallace, 1865) stat. rev.
  L. doubledayi cacharensis (Butler, 1885) comb. nov.
  Papilio cacharensis (Butler, 1885); Ann. Mag. Nat. Hist. (Ser. 5), 16: 344; TL: “Near Assam … Cachar” [Cachar, Assam, N.E. India].
  Distribution: Assam and Meghalaya, N.E. India.
  L. doubledayi putaoa (Tytler, 1939) comb. rev.
  Polydorus doubledayi putaoa (Tytler, 1939); J. Bomb. nat. Hist. Soc., 41(2): 236; TL: “Putao, N.-E. Burma” [Putao, Kachin State, North Myanmar].
  Distribution: Upper Irrawaddy River valley, N. Myanmar.
  L. doubledayi sambilanga (Doherty, 1886) comb. rev.
  Papilio doubledaii[sic] var. sambilanga (Doherty, 1886); J. asiat. Soc. Bengal (Pt. II), 55(3): 263; TL: “Great Nicobar”.
  Distribution: Nicobar Islands [India].
  L. doubledayi delianus (Fruhstorfer, 1895) comb. rev.
  Papilio doubledayi var. delianus (Fruhstorfer, 1895); Ent. Nachr., 21(12/13): 196; TL: “Deli, Sumatra” [Medan, N. Sumatra, Indonesia].
  Distribution: Northeastern and northern Sumatra [Indonesia].
  L. doubledayi doubledayi (Wallace, 1865)
  Papilio Doubledayi (Wallace, 1865); Trans. linn. Soc. Lond., 25: 42; TL: “Moulmein, Assam” [Mawlamyine, southern Myanmar and Assam, N. E. India].
     Polydorus doubledayi merguia (Tytler, 1939); J. Bomb. nat. Hist. Soc., 41(2): 236; TL: “Victoria Pt., Mergui” [Myeik, Tanintharyi, southern Myanmar].
  Distribution: Malay Peninsula, southern Myanmar, Thailand, Laos, Cambodia, Vietnam.
  L. doubledayi insperatus (Joicey and Talbot, 1921) comb. nov.
  Papilio coon insperatus (Joicey and Talbot, 1921); Bull. Hill Mus. Witley, 1(1): 168, pl. XIX, f. 2; TL: “Interior, Hainan”.
  Distribution: Hainan Is. [China].

4. Discussion

Our findings showed that L. coon and L. doubledayi are two distinct species within the genus Losaria. The molecular data suggested that L. coon is sister to L. rhodifer, which are then sister to L. doubledayi, as shown in Figure 2. Morphological analyses of genitalic structures and wing patterns are consistent with the molecular evidence, as shown in Figure 3, Figure 4, Figure 5, Figure 6, Figure 7 and Figure 8; therefore, we confirm the species status of L. doubledayi. Furthermore, our morphological analysis, based on the literature [2] and type photos of all recognised subspecies of L. coon and L. doubledayi, allowed us to update the subspecies list of each species, with L. coon, containing four subspecies, and L. doubledayi, containing six subspecies. As stated under Materials and Methods (Taxon sampling), the present study was unable to include all subspecies due to difficulties in obtaining specimens from India, northern Myanmar and some Indonesian islands, and consequently partial findings can be presented here for subspecies. Future research must address this point to form a better understanding of the subspecies divergence, diversity, and distribution pattern when those taxa become accessible.
The divergence between L. coon coon and L. coon sangkapurae is limited, as shown in Table 2 and Table 3 and Figure 2, despite the two subspecies being isolated on different islands. Geographic proximity and recent separation between Sumatra, Java, and those offshore small islands might explain such limited divergence [40,41,42,43]. Based on the distribution pattern and morphological characters of the other two subspecies [2], namely ssp. palembanganus and ssp. patianus, it is likely that the divergence between them would also be limited.
In comparison, the divergence within L. doubledayi is greater. The phylogenetic tree, genetic distances and molecular species delimitation results showed that even the populations from the Malay Peninsula and upper Indochina are different, let alone the insular ssp. insperatus from Hainan, as shown in Table 3 and Figure 2. Our examination of a large series of specimens, type photos, and literature records also showed morphological differences among these populations/subspecies. For instance, populations from upper Indochina are usually smaller in size and possess larger hindwing white discal patches compared to populations from the Malay Peninsula, and the population from South Vietnam (samples LCN018 and LCN019 in our dataset, other examined specimens, and also in the cited literature) possesses unique ochreous abdomens, rather than crimson ones [44]. Similarly, photos of live specimens of ssp. cacharensis, plus type photos and the description of ssp. putaoa also showed constant morphological differences compared to other populations [14,45]. Future research should address the question of the population divergence of L. doubledayi across its distribution range.
The molecular and morphological divergence within L. doubledayi across its range might be attributed to two factors. The first factor lies in the more heterogeneous natural environment (e.g., terrain topology, climate, and landscape). The valley habitat in North Myanmar and Northeast India, separated by mountains, where ssp. putaoa and ssp. cacharensis, respectively, occur, is a typical example of divergence. Such extreme altitude shifts restricted populations within narrow ranges (mostly river valleys with broadleaf forest patches), and they subsequently became distinct evolutionary entities (subspecies). Similarly, the southern–central savannah plain in Thailand and Cambodia formed an effective barrier due to the lack of suitable forests for the larval foodplants. The second factor is the larval foodplants for Losaria, which constitute a handful of species in the genus Thottea (family Aristolochiaceae) [46]. Thottea is a genus of lowland forest-dwelling shrub species with high endemism; habitats without dense forests would be unsuitable for Losaria [47,48]. The superimposition of both factors resulted in spatially fragmented suitable habitats, which eventually lead to divergence within L. doubledayi.
Given the separation of L. coon and L. doubledayi, conservation strategy and threat evaluation of the original L. coon should be reassessed. According to Fernando, Jangid, Chowdhury, Kehimar, Lo, and Moonen [12], L. coon and L. doubledayi were considered as a single wide-ranging species, with an IUCN rank of Least Concern (LC). After its work separating these two species, L. coon now becomes a narrow-ranged species that only occurs in several Indonesian islands, including southern Sumatra, Java, and Bawean, as shown in Figure 1. Hence, conservation and assessment strategies must be updated accordingly to ascertain the current population status. Furthermore, as a separate species, although L. doubledayi occupies a much wider range, it still possesses more localised subspecies, even within continental Indochina, as shown in Figure 1 and Figure 2. Each population represents a distinct evolutionary pool, while some of them only occupy a much smaller geographic range than others (e.g., ssp. cacharensis and ssp. putaoa). Since anthropogenic disturbance to most of its distribution range remains active to date [49,50,51,52,53], we think that the population status, vulnerability, and threat factors of each subspecies of L. doubledayi must be re-evaluated for future conservation.

5. Conclusions

The present study used DNA barcode data and morphological characters to unveil the relationship of the yellow and red marked Losaria coon across its distribution range. Our analyses support the separation of L. coon (yellow marked) and L. doubledayi (red marked) as two distinct species. Our findings also updated the geographic ranges of the two species: with L. coon restricted to southern Sumatra, Java, and Bawean Island, while L. doubledayi occurs widely in regions from North India to northern Sumatra, including Hainan and Nicobar Islands. The separation effectively made L. coon a narrow-ranged species which may require more conservation attention in the future, while that for L. doubledayi must also be revised due to its higher subspecies diversity reflecting multiple evolutionary pools associated with larval foodplant restriction and landscape heterogeneity.

Author Contributions

Conceptualization, S.-J.H. and A.M.C.; methodology, S.-J.H., F.L.C, and Y.-Y.W.; investigation: Z.-B.X. and Y.-Y.W.; resources, S.-J.H. and A.M.C.; data curation, Z.-B.X., S.-J.H., and Y.-Y.W.; writing—original draft preparation, Z.-B.X.; writing—review and editing, S.-J.H., A.M.C., F.L.C., and Z.-B.X.; supervision, S.-J.H.; funding acquisition, S.-J.H. All authors have read and agreed to the published version of the manuscript.

Funding

This study was supported by the NSFC Programme of China (41761011) and the Biodiversity Conservation Programme of the Ministry of Ecology and Environment, China (China-BON Butterflies) (SDZXWJZ01013). F.L.C. has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (project GAIA, agreement No. 851188).

Acknowledgments

The authors wish to thank Barcode of Life (Ontario, Canada) for sequencing some among the museum specimens for this study. We also thank Philip Yik-Fui Lo (Kadoorie Farm and Botanic Garden, Hong Kong, China), Tian-Zhu Xiong (Harvard University, USA), and Hui-Hong Zhang (Yunnan University, Kunming, China) for sample collection and analytical assistance. Additionally the authors wish to thank: the Trustees of the Natural History Museum (BMNH), London, UK, for their assistance in accessing the BMNH collection; Gerardo Lamas (Museo de Historia Natural, Lima, Peru) for the information provided; Yutaka Inayoshi (Chiang Mai, Thailand) for access to his collection; Maurizio Bollino (Lecce, Italy) for providing papers; Jan Moonen (Cadier en Keer, Netherlands) and Krushnamegh Kunte (Bangalore, India) for sharing photographs of specimens; Christoph Häuser and Axel Steiner (Stuttgart, Germany) for providing photographs of type specimens under the GART/GloBis project.

Conflicts of Interest

The authors declare no conflict of interest.

Appendix A. List of Losaria Specimens Examined, Different Labels in Museum Specimens Are Separated with a ‘/’

Names of depositories are given in alphabetical order, with institutions listed after private collections, and are abbreviated as follows: AMC: collection of Adam M. Cotton (Chiang Mai, Thailand); SJH: collection of Shao-Ji Hu (Kunming, China); YI: collection of Yutaka Inayoshi (Chiang Mai, Thailand); PYFL: collection of Philip Yik-Fui Lo (Kadoorie Farm and Botanic Garden, Hong Kong, China); TR: collection of Tommaso Racheli (Rome, Italy); HHZ: collection of Hui-Hong Zhang (Kunming, China). [OD]: illustrated in the original description; [T&N]: illustrated in Tsukada and Nishiyama, 1980. BMNH: collections of the Natural History Museum (London, United Kingdom); MNHN: collection of Muséum National d’Histoire Naturelle (Paris, France); NBC: collections of the Naturalis Biodiversity Center (Leiden, The Netherlands); ZSSM: collection of Zoologische Staatssammlung, München (Munich, Germany).
Losaria palu (Martin, 1912)
1♀, HOLOTYPE, Lewara, bei Palue, 26. V. 12/Type Pap. palu Martin/Sammlung L. Martin Celebes c. Palu, Lewara 26. V. 12/[red label] Cotypus ♀ Papilio palu Martin Zool. Staatssammlung München [ZSSM]; 1♀, N. W. Celebes Lewara bei Palue, VIII. 12/Type Pap. palu Mart./Sammlung L. Martin Celebes c. Palu, Lewara VIII. 12/[red label] Cotypus ♀ Papilio palu Martin Zool. Staatssammlung München [note, this specimen is not a type] [ZSSM]; 1♂, Palu, Sulawesi, 1983–IV–08 [NBC]; 1♀, Palu, Sulawesi, 1983–IV–08 [NBC]; 1♂, Palu, Sulawesi, 1987–III–04 [NBC]; 1♂, W. Palu, C. Sulawesi, Sahudini leg. 1983–VI–15 [AMC]; 1♂, 1♀, W. Palu, C. Sulawesi, 1983–IV–14 [AMC]; 1♀, W. Palu, C. Sulawesi, 1983–IV–20 [AMC]; 1♂, W. Palu, C. Sulawesi, Nakamoto and Detani leg. 1983–III–07 [AMC]; 1♀, W. Palu, C. Sulawesi, Nakamoto and Detani leg. 1983–III–13 [AMC]; 1♂, Palolo, Palu, C. Sulawesi, ex David Cassat 2000–III [AMC]; 1♂, Palu, Sulawesi, ex Hadi Hendra 1998–V [AMC]; 1♂, Palu, Sulawesi, ex Hadi Hendra 1998–X [AMC]; 1♂, Palu, Sulawesi, ex Hadi Hendra 1999–IV [AMC]; 1♂, Donggala, C. Sulawesi, 2017–II [SJH]; 1♂, Donggala, C. Sulawesi, 2017–X [SJH]; 1♀, Donggala, C. Sulawesi, 2018–I [SJH].
Losaria neptunus manasukkiti (Cotton, Racheli and Sukhumalind, 2005)
1♀ HOLOTYPE, Kam Phuan, Ranong, Thailand, K. Ruengrith leg., 2005–I–02 [TR]; 1♂ PARATYPE, Kampuan Ranong Thailand Y. Inayoshi leg. 1988–III–28 [YI]; 1♀ PARATYPE, Kampuan Ranong Thailand Y. Inayoshi leg. 1991–III–20 [YI]; 1♂ PARATYPE, Muang Chone Ranong Thailand Y. Inayoshi leg. 1984–III–28 [YI]; 1♂ PARATYPE, Muang Chone Ranong Thailand S. Ruangrit leg. 1995–IV–05 [YI]; 1♂ PARATYPE, Muang Chone Ranong Thailand S. Ruangrit leg. 1995–IV–06 [YI]; 1♀ PARATYPE, Muang Chone Ranong Thailand S. Ruangrit leg. 1992–IV–14 [YI]; 1♂ PARATYPE, Kamphuan, Kapoe, Ranong Prov. Thailand P. Sukkit leg. 1989–I–23 [AMC]; 1♂ PARATYPE, Kamphuan, Kapoe, Ranong Prov. Thailand Prasobsuk Sukkit leg. 1989–IV–20 [AMC]; 1♂ PARATYPE, Kamphuan, Kapoe, Ranong Prov. Thailand P. Sukkit leg. 1989–IV–29 [AMC]; 1♂ PARATYPE, Kamphuan, Kapoe, Ranong Prov. Thailand P. Sukkit leg. 1989–III [AMC]; 1♂, 1♀ PARATYPE, Klong Na Kha, Ranong Province, Thailand Prasobsuk Sukkit leg. 1980–VI–23-26 [AMC]; 1♂ PARATYPE, Kamphuan, Kapoe, Ranong Prov. Thailand Prasobsuk Sukkit leg. 1988–I–19 [AMC]; 1♂ PARATYPE, Kamphuan, Kapoe, Ranong Prov. Thailand Prasobsuk Sukkit leg. 1988–I–28 [AMC]; 1♀ PARATYPE, Klong Na Kha, Ranong Province, Thailand Prasobsuk Sukkit leg. 1979–III–31 [AMC]; 2♂ PARATYPES, Hot Springs, Ranong, Thailand Mana Sukkit leg. 1977–X–19 [AMC]; 1♂ PARATYPE, Klong Nakha, Ranong Province, Thailand Kriangkrai Ruengrith leg. 2004–XII–30 [AMC]; 1♀ PARATYPE, Kam Phuan, 90 km south of Ranong, Thailand 1988–IV–30 [AMC]; 1♂, Kam Phuan, 90 km south of Ranong, Thailand ex Prasobsuk Sukkit 1988–III–28 [AMC]; 1♀ Suksamran, Kapoe, Ranong Province, Thailand ex Prasobsuk Sukkit 2004–III–27 [AMC].
Losaria neptunus neptunus (Guérin-Méneville, 1840)
1♂ SYNTYPE, [illegible handwriting]/Typicum Specimen/EX-MUSÆO Dris. BOISDUVAL/Levick Bequest B. M.1941-83./BMNH(E) # 149290 [BMNH]; 1♀ SYNTYPE, Pap. Neptunus guérin [illegible handwriting]/neptunus guérin Voyage de Delessert, pl. 19. Pulopinang/Typicum Specimen/EX-MUSÆO Dris. BOISDUVAL/Levick Bequest B. M.1941-83./BMNH(E) # 149291 [BMNH]; 1♀, Phrom Lok Waterfall, Nakhon Sri Thammarat, Thailand Y. Inayoshi leg. 1992–III–14 [YI]; 3♂, Than To, Yala, Thailand Y. Inayoshi leg. 1992–III–18-20 [YI]; 1♂ Khao Chong, Trang Province, Thailand Prasobsuk Sukkit leg. 1983–I–20 [AMC]; 1♀ Than To Waterfall, Yala Province, Thailand Prasobsuk Sukkit leg. 1992–II–19 [AMC]; 1♀ N of Betong, 787 m., Yala Prov., Thailand 5° 53.865’ N, 101° 2.344’ E Prasobsuk Sukkit leg. 2014–VI–19 [AMC]; 1♂, 1♀ Tapah, W. Malaysia 1978–V [AMC]; 1♂, 2♀ Tapah, Perak, W Malaysia 1987–XII [AMC]; 1♀ Hot springs, 1000 ft., seven miles from Tapah, W. Malaysia A. M. Cotton leg. 1979–VII–31 [AMC]; 1♂, 1♀, Ipoh, W. Malaysia 1979–II [T&N].
Losaria neptunus creber (van Eecke, 1914)
3♀, Simeulue, ex David Cassat 2005–VII [AMC]; 3♂, Simeulue, ex Andrew Rawlins 2005–IX [AMC]; 2♀, Simeulue, ex David Cassat 2005–IX [AMC]; 1♀, Simeulue, ex Andrew Rawlins [AMC]; 1♂, Simeulue, ex David Cassat 2006–III [AMC]; 1♂, 2♀, E. Simeulue, ex Hadi Hendra 2006–III [AMC]; 1♂, E. Simeulue, ex Hadi Hendra 2006–VII [AMC]; 1♂, Simeulue Is., W. Sumatra [T&N]; 2♂, 2♀, Simeulue, 2010–VIII [SJH].
Losaria neptunus fehri (Honrath, 1892)
1♂ SYNTYPE, Ins Nias/var fehri Honr/Ex specimini- bus typicis/[red edged round label] Type [BMNH]; 2♂, 2♀, Nias, ex Andrew Rawlins 1997–V [AMC]; 2♂, 4♀, S. Nias, ex Hadi Hendra 2002–IV [AMC]; 1♂, Nias [T&N]; 1♂, Gunongsitoli, N. Nias 1957–V [T&N]; 1♀, Tehemberau, N. Nias 1979–V–23 [T&N]; 1♀, Nias, 1991–II–8 [SJH].
Losaria neptunus lepida (Hanafusa, 1994)
1♂ HOLOTYPE, Tanahmasa Is., Batu Islands, Indonesia 1994–V [OD]; 1♀, Tanahmasa, Batu Is. ex David Cassat 2007–V [AMC].
Losaria neptunus eminens (Hanafusa, 1990)
1♂ HOLOTYPE, Sipora Island, Kep. Mentawai, Indonesia 1989–V–28 [OD]; 1♀ PARATYPE, Sipora Island, Kep. Mentawai, Indonesia 1989–V [OD]; 1♀ Siberut ex Andrew Rawlins 2005–VII [AMC]; 1♀ Siberut ex Andrew Rawlins [AMC].
Losaria neptunus siborangitana (Tsukada and Nishiyama, 1980)
1♂, 1♀ PARATYPE, Kalo Hill, Sumatra 1977–III [T&N]; 1♂ PARATYPE, Kalo Hill, Sumatra 1979–III [T&N]; 1♀, Sumatra Oost Kust 1912 186 Coll. v. d. Bergh. Sibolangit ♀ Papilio neptunus Guérin/Collectie Zoölogisch Museum Amsterdam/Losaria neptunus sumatranum (Hagen) [= ssp. neptunus in my revision] det. J.D. Weintraub, 1990/♀ Atrophaneura (Losaria) neptunus siborangitana Ts. and N. det. J. Moonen, 2013 [NBC]; 1♀, Brastagi, N. Sumatra, ex Andrew Rawlins 2005–VI [AMC].
Losaria neptunus padanganus (Rothschild, 1908)
1♂ SYNTYPE, Padang Sidem- poean, W. Sum. (Ericsson)./P. neptunus padanganus Type Rothsch. Nov. Zool. 1908 p. 165 [BMNH]; 1♀, Sumatra West Kust 1912 45 Coll. v. d. Bergh. Si. Matorkis ♀ Papilio neptunus Guérin [back] zelf gerangen Mei 1912/Collectie Zoölogisch Museum Amsterdam/Losaria neptunus sumatranum (Hagen) [= ssp. neptunus in my revision] det. J.D. Weintraub, 1990/♀ Atrophaneura (Losaria) neptunus siborangitana Ts. and N. det. J. Moonen, 2013 [NBC]; 1♂, Jambi, C. Sumatra 2005–VII [AMC]; 1♀, Anai Valley, 350 m., 10 km. s. of Padang Panjang, W. Sumatra Leo Liang leg. 1979–VIII–7 [AMC]; 1♀, Anai Valley, 350 m., 10 km. s. of Padang Panjang, W. Sumatra Leo Liang leg. 1979–VIII–15 [AMC]; 1♀, Anai Valley, 350 m., 10 km. South of Padang Panjang, W. Sumatra ex Andrew Rawlins 2004–IV [AMC]; 1♂, Ujuug[sic] Rejo, W. Sumatra 1979–X [T&N]; 1♀, Lembah Anai, W. Sumatra 1976–XII [T&N].
Losaria neptunus doris (Rothschild, 1908)
1♂ SYNTYPE, N. Borneo/P. neptunus doris Rothsch. Type. Nov. Zool. 1908 p. 165. [BMNH]; 1♂, Mt. Kinabalu, N. Borneo 1962–V [T&N]; 1♂, Sibatik Is., N.E. Borneo 1962–II–10 [T&N]; 1♂, Mt. Kapari, S. Borneo 1973–III–16 [T&N]; 1♀, Mt. Kapari, S. Borneo 1973–III–21 [T&N]; 1♂, W. Kalimantan, 2018–I [SJH].
Losaria neptunus dacasini (Schröder, 1976)
1♂ HOLOTYPE, Philippinen, Palawan D. and G. Dacasin leg. 1975–VI–16 [OD]; 4♂, 4♀, Roxas, Palawan, ex Coll. Greg Watson 1980–IV–14-18 [AMC]; 1♂, 1♀, N. Palawan 1983–XII [AMC]; 1♂, 1♀, N. Palawan, G. Dacasin leg. ex Roger de Keyser 1984–IX [AMC]; 2♂, 3♀, Barangay Magara, 20m., Roxas, Palawan, Philippines 2014–X [AMC]; 1♂, 1♀, Palawan 1979–IV [T&N]; 1♂, 1♀, Palawan, 2015–VIII–25 [SJH].
Losaria neptunus matbai (Schröder and Treadaway, 1990)
1♀ HOLOTYPE, Philippinen, Sulu-Archipel, Tawitawi-Gruppe C. G. Treadaway leg. 1990–VI–09 [OD]; 1♂, 1♀, Tawi-Tawi, Philippines, ex Nishiyama 2007–IV [AMC]; 3♂, 3♀, Tawi-Tawi, Philippines, ex David Cassat 2007–V [AMC].
Losaria coon palembanganus (Rothschild, 1896)
1♂ SYNTYPE, Musi R., Palembang distr. 103° E Long 3° S Lat/P. coon palembanganus Type! Rothsch. Nov. Zool. 96. [BMNH]; 1♂, 1♀, Kurui, S. Sumatra 1979–IX [T&N].
Losaria coon coon (Fabricius, 1793)
1♀ SYNTYPE [of Papilio hypenor Godart, 1819], TYPE/MUSÉUM PARIS/3/type de Godart/This specimen to be designated LECTOTYPE of Papilio hypenor Godt. det. J.D. Weintraub, 1990/[purple edged round label] LECTO-TYPE [not designated] [MNHN]; 1♂, WESTJAVA MT.SALAK 850M 5-6-1986 leg. G. LAM/INDONESIA W. Java, Mt. Salak. 850 m 5. VI. 1986 leg. G. Lam/Museum Leiden Collectie RMNH/♂ Atr. (Losaria) coon coon (Fabricius) det. J.Moonen, 2014 [NBC]; 1♀, INDONESIA W. Java, Mt. Salak. 850 m 4. IV. 1986 leg. G. Lam/Museum Leiden Collectie RMNH/♀ Atr. (Losaria) coon coon (Fabricius) det. J.Moonen, 2014 [NBC]; 1♂, INDONESIA, W. Java, Bogor. (1989)/collectie J.Moonen./Pachl. (Losaria Moore) coon Fabricius ♂ coon Fabricius det. J.Moonen [NBC]; 2♂, Gunung Gede, nr. Bogor, W. Java 1975–VI [AMC]; 1♀, Gunung Gede, nr. Bogor, W. Java 1975–VIII [AMC]; 1♀, Cilaungsi, Ciawi, Bogor, W. Java Jarujin Nabhitabhata leg. 1977–VI–17 [AMC]; 1♂, W. Java ex Coll. Prasobsuk Sukkit 1981–VII–21 [AMC]; 2♀, Cianjur, W Java, ex Hasan 2004–V [AMC]; 2♂, 4♀, Cianjur, W Java, ex Hadi Hendra 2004–X [AMC]; 2♂, Cianjur, W Java, ex Hasan 2005–V [AMC]; 1♂, 1♀, Mt. Sarak[sic], W. Java 1972–IX [T&N]; 1♂, Mt. Gede, W. Java 1892–VIII [T&N]; 4♂, 4♀, W. Java, 2018–I [SJH].
Losaria coon patianus (Fruhstorfer, 1898)
[no specimens or photos available for examination]
Losaria coon sangkapurae (Bollino and Sala, 1992)
1♂ HOLOTYPE, Pulau Bawean [OD]; 1♂, 1♀ PARATYPE, Pulau Bawean [OD]; 1♂, 1♀, Bawean Is., 2018–II [SJH].
Losaria rhodifer (Butler, 1876)
1♀ HOLOTYPE, Andaman Islands [back] 1497.47./Andaman Is. Druce Coll./♀/Papilio rhodifer Butl. Godman-Salvin Coll. 1918.—4./B. M. TYPE No. Rh.10869. Papilio rhodifer. ♀ Butl./P. rhodifer Butler Type/[red edged round label] Type H. T./[BMNH]; 1♂, Andamans Hewitson coll. 79.69 Papilio coon .3./BMNH(E) # 986165 [BMNH]; 1♀, Andaman Is. VII. 1923. G. G. Field. B.M.1924-351./BMNH(E) # 986273 [BMNH]; 1♂, S. Andaman 1984–II–19 [AMC]; 1♂, Andaman Is. Eric Khoo leg., ex Nishiyama 2007–V [AMC]; 1♀, Andaman Is. Eric Khoo leg., ex Nishiyama 2007–V–07 [AMC]; 1♂, Port Blair, S. Andaman 1979–X–13 [T&N]; 1♀, Port Blair, S. Andaman 1979–XI [T&N]; 1♂, Andaman Is., 2014–X [SJH]; 1♀, Andaman Is., 1962–III [SJH].
Losaria doubledayi cacharensis (Butler, 1885)
1♂ SYNTYPE, Assam 85. 69 [back] Papilio cacharensis ♂ type Butler/B. M. TYPE No. Rh.10864. Papilio cacharensis ♂ Butl./[red edged round label] Type/BMNH(E) # 149293 [BMNH]; 1♀ SYNTYPE, Cachar 78. 70/B. M. TYPE No. Rh.10865. Papilio cacharensis ♀ Butl./[red edged round label] Type/BMNH(E) # 149294 [BMNH]; 1♂, ♂ Lengba R Manipur E. 00 4 13/H. C. Tytler Coll. B.M.1941-92./BMNH(E) #986210 [BMNH]; 1♀, Irang Riv Manipur E 10 00 3 . 14/H. C. Tytler Coll. B.M.1941-92./BMNH(E) #986369 [BMNH].
Losaria doubledayi putaoa (Tytler, 1939)
1♀ SYNTYPE, ♀ Putao Rd. N. E. Burma 4. 27/P. doubledayi putaoa s. sp. nov./[red edged round label] Type HT/BMNH(E) # 149292 [BMNH].
Losaria doubledayi sambilanga (Doherty, 1886)
[no specimens or photos available for examination]
Losaria doubledayi delianus (Fruhstorfer, 1895)
1♂ SYNTYPE, Sumatra Deli ex coll. Fruhstorfer [back] Döhrn/[black edged rectangle] delianus Fruhst./Fruhstorfer Coll. B.M. 1933-131. [BMNH]; 1♂, Gayoe Mts., N. E. Sum., V-93 Dr. Martin. [BMNH]; 1♂, 1♀, Gedong Biara, Sumatra 1952–IV–20 [T&N].
Losaria doubledayi doubledayi (Wallace, 1865)
1♂ SYNTYPE, Moulmein. Pur. from Arch. Clark 43 — 43. 549d/[round label] 43 43/[round label] 549 d/[purple edged round label] LECTO-TYPE [BMNH]; 1♂ SYNTYPE [of Polydorus doubledayi merguia (Tytler, 1939)], ♂ Pt. Victoria 1. 19/doubledayi merguia Tytl./[red edged round label] Type HT/BMNH(E) # 149295 [BMNH]; 1♀ SYNTYPE [of Polydorus doubledayi merguia (Tytler, 1939)], ♀ Victoria Pt 12. 25/[red edged round label] Type AT/BMNH(E) # 149296 [BMNH]; 1♂ Phong Saly, Laos KP 2012–VIII–17 [YI]; 1♂, Wang Chin, Phrae, Thailand 1988–I–29 [YI]; 1♀, Phu Pahn, Mukdaharn, Thailand 1986–V–24 [YI]; 1♂, 1♀, Klong Nakha, Ranong, Thailand Y. Inayoshi leg. 1984–II–27 [YI]; 1♂, Bo Nam Ron, Ranong, Thailand Y. Inayoshi leg. 1983–IV–17 [YI]; 1♀, Wang Chin, Phrae Prov., Thailand 1983–X–25 [AMC]; 2♂, Wang Chin, Phrae Prov., Thailand 1987–XI–16 [AMC]; 1♂, Wang Chin, Phrae Prov., Thailand 1999–II–24 [AMC]; 2♀, Wang Chin, Phrae Prov., Thailand 2006–III–10 [AMC]; 4♂, Wang Chin, Phrae Prov., Thailand 2006–III–08 [AMC]; 1♂, 1♀, Wang Chin, Phrae Prov., Thailand 2006–V–01 [AMC]; 1♀, Wang Chin, Phrae Prov., Thailand 2006–V–21 [AMC]; 2♀, Wang Chin, Phrae Prov., Thailand 2006–VI–3-4 [AMC]; 1♂, Wang Chin, Phrae Prov., Thailand 2007–II–28 [AMC]; 1♂, Wang Chin, Phrae Prov., Thailand 2007–XII–11 [AMC]; 1♂, Wang Chin, Phrae Prov., Thailand 2008–III–13 [AMC]; 1♂, Wang Chin, Phrae Prov., Thailand 2008–VI–26 [AMC]; 1♀, Phu Wua, Nong Khai Province, Thailand Mana Sukkit leg. 1979–X–17 [AMC]; 1♂, Phu Wua, Nong Khai Province, Thailand Mana Sukkit leg. 1984–XII–22 [AMC]; 1♀, 1 km west of Ban Koun Ngun, 177 m., Hinboun District, Laos 18° 09.0’ N, 104° 26.7’ E A. M. Cotton leg. 2005–VII–31 [AMC]; 1♂, viewpoint, 460 m., Phu Pha Man, Hinboun Dist., Laos 18° 10.7’ N, 104° 29.0’ E A. M. Cotton leg. 2008–VII–28 [AMC]; 1♀, viewpoint, 470 m., Phu Pha Man, Hinboun Dist., Laos 18° 10.7’ N, 104° 29.1’ E A. M. Cotton leg. 2005–VIII–02 [AMC]; 1♀, viewpoint, 470 m., Phu Pha Man, Hinboun Dist., Laos 18° 10.7’ N, 104° 29.1’ E A. M. Cotton leg. 2007–VIII–08 [AMC]; 1♂, 1♀, east slope, 423 m., Phu Pha Man, Hinboun Dist., Laos 18° 11.1’ N, 104° 29.3’ E A. M. Cotton leg. 2008–VII–30 [AMC]; 1♂, east slope, 423 m., Phu Pha Man, Hinboun Dist., Laos 18° 11.1’ N, 104° 29.3’ E A. M. Cotton leg. 2008–VII–31 [AMC]; 1♀, east slope, 386 m., Phu Pha Man, Hinboun Dist., Laos 18° 11.1’ N, 104° 29.4’ E A. M. Cotton leg. 2008–VII–31 [AMC]; 1♀, viewpoint, 460 m., Phu Pha Man, Hinboun Dist., Laos 18° 10.7’ N, 104° 29.0’ E A. M. Cotton leg. 2008–VIII–02 [AMC]; 1♀, east slope, 413 m., Phu Pha Man, Hinboun Dist., Laos 18°11.2’ N, 104° 29.3’ E A. M. Cotton leg. 2008–VIII–07 [AMC]; 1♀, east slope, 425 m., Phu Pha Man, Hinboun Dist., Laos 18°11.0’ N, 104° 29.3’ E A. M. Cotton leg. 2008–VIII–07 [AMC]; 1♀, east slope, 425 m., Phu Pha Man, Hinboun Dist., Laos 18°11.0’ N, 104° 29.3’ E A. M. Cotton leg. 2008–VIII–12 [AMC]; 1♀, Tad Nam Sanam Stream, 182 m., Koun Kham valley, Hinboun Dist., Laos 18° 12.9’ N, 104° 31.0’ E Khamboun SP. leg. 2008–III–04 [AMC]; 1♂, Khao Soi Dao, Chantaburi Province, Thailand Paopong Wijitponglagen leg. 1979–VII [AMC]; 1♂, Klong Ai Saw, Pattawi, Makham, Chantaburi, Thailand Mana Sukkit leg. 1988–IX–12 [AMC]; 1♀, Klong Ai Saw, Pattawi, Makham, Chantaburi, Thailand Mana Sukkit leg. 1989–IX–12 [AMC]; 1♂, Klong Ai Saw, Pattawi, Makham, Chantaburi, Thailand ex Prasobsuk Sukkit 2009–VII–03 [AMC]; 5♂, 1♀, Trapeang Rung, 20 m., s of Koh Kong, SW Cambodia Sergey Murzin leg. 2008–XII–20-27 [AMC]; 1♀, Ranong, Thailand Bro. Amnuay Pinratana leg. 1976–X [AMC]; 1♂, Ranong, Thailand Prasobsuk Sukkit leg. 1983–III–31 [AMC]; 1♂, 1♀, Hot Springs, Ranong, Thailand Mana Sukkit leg. 1977–X–20 [AMC]; 1♂, Hot Springs, Ranong, Thailand A. M. Cotton leg. 1984–II–26 [AMC]; 3♂, Hot Springs, Ranong, Thailand Prasobsuk Sukkit leg. 2013–IV–06 [AMC]; 1♀, Suksamran, Ranong Province, Thailand ex Prasobsuk Sukkit 1996–II–24 [AMC]; 1♂, Suksamran, Ranong Province, Thailand ex Prasobsuk Sukkit 1996–IV–10 [AMC]; 1♂, Suksamran, Ranong Province, Thailand ex Prasobsuk Sukkit 1996–IV–17 [AMC]; 1♀, Suksamran, Ranong Province, Thailand ex Prasobsuk Sukkit 2004–III–11 [AMC]; 1♂, Suksamran, Ranong Province, Thailand ex Prasobsuk Sukkit 2004–III–22 [AMC]; 1♂, Suksamran, Ranong Province, Thailand ex Prasobsuk Sukkit 2004–III–29 [AMC]; 1♀, Suksamran, Ranong Province, Thailand ex Prasobsuk Sukkit 2004–III–30 [AMC]; 2♂, Kamphuan, Kapoe, Ranong Province, Thailand Prasobsuk Sukkit leg. 1986–I–19 [AMC]; 3♂, Kamphuan, Kapoe, Ranong Province, Thailand Prasobsuk Sukkit leg. 1986–I–28 [AMC]; 1♂, Kamphuan, Kapoe, Ranong Province, Thailand S. Ruangrit leg. 1989–I–19 [AMC]; 3♂, Kamphuan, Kapoe, Ranong Province, Thailand Prasobsuk Sukkit leg. 1989–I–20 [AMC]; 1♂, Kamphuan, Kapoe, Ranong Province, Thailand Prasobsuk Sukkit leg. 1989–I–23 [AMC]; 1♂, Kamphuan, Kapoe, Ranong Province, Thailand Prasobsuk Sukkit leg. 1989–IV–21 [AMC]; 1♂, Kamphuan, Kapoe, Ranong Province, Thailand C. Taruishi leg. 1989–IV–23 [AMC]; 1♂, Kamphuan, Kapoe, Ranong Province, Thailand Prasobsuk Sukkit leg. 1989–IV–24 [AMC]; 1♂, Kamphuan, Kapoe, Ranong Province, Thailand Mana Sukkit leg. 1989–VIII–15 [AMC]; 2♂, Kamphuan, Kapoe, Ranong Province, Thailand Prasobsuk Sukkit leg. 1992–II–27 [AMC]; 1♂, 1♀, Klong Na Kha, Ranong Province, Thailand Prasobsuk Sukkit leg. 1980–VI–22 [AMC]; 1♂, Klong Na Kha, Ranong Province, Thailand Prasobsuk Sukkit leg. 1980–VI–23-26 [AMC]; 1♂, Kathu Waterfall, Phuket, Thailand A. M. Cotton leg. 1986–XI–08 [AMC]; 1♂, forested Mt near Coral Beach Hotel, Patong, Phuket, Thailand Bob Borth leg. 1995–XI–23 [AMC]; 2♂, Koh Lanta, Krabi Province, Thailand Prasobsuk Sukkit leg. 1992–II–23 [AMC]; 1♀, Khao Hang Nak, Krabi, Thailand Prasobsuk Sukkit leg. 2004–II–21 [AMC]; 1♀, Khao Chong, Trang Province, Thailand Mana Sukkit leg. 1981–I–06 [AMC]; 1♀, Khao Chong, Trang Province, Thailand Mana Sukkit leg. 1985–I–13 [AMC]; 1♂, Khao Chong, Trang Province, Thailand Prasobsuk Sukkit leg. 1988–XII–31 [AMC]; 1♂, Khao Chong, Trang Province, Thailand Prasobsuk Sukkit leg. 1989–I–06 [AMC]; 1♂, Thaleban, Satun Province, Thailand Prasobsuk Sukkit leg. 1997–III–29 [AMC]; 2♂, Thaleban, 100m., Satun, Thailand 6° 42.55′N, 100° 10.23′E Prasobsuk Sukkit leg. 2014–IV–16 [AMC]; 3♂, Phong Phong Waterfall, Pattani Province, Thailand Prasobsuk Sukkit leg. 1997–III–19 [AMC]; 1♂, Betong, Yala Prov., S. Thailand Prasobsuk Sukkit leg. 2014–VII–26 [AMC]; 2♂, 2♀, Tapah, Perak, W. Malaysia 1987–XII [AMC]; 1♀, Tapah Hills, Perak, W. Malaysia ex Michael Yeh 2010–II [AMC]; 1♂, 1♀, Ulu Piah, W. Malaysia 1979–XII [T&N]; 6♂, Ranong, S. Thailand, 2018–II [SJH]; 3♂, Wang Chin, Phrae, Thailand, 2017–XI–11 [SJH].
Losaria doubledayi insperatus (Joicey and Talbot, 1921)
1♂, SYNTYPE, 47.2I./69. 20 Hainan Inter. July 1920. C. T. Bowring./Presented by J.J.Joicey Esq. Brit. Mus.1931-291./P. coon insperatus J. and T./[red edged round label] Type HT/BMNH(E) # 149297 [BMNH]; 1♀, SYNTYPE, 89. 20 Interior Hainan, September 1920. C. T. Bowring./Presented by J.J.Joicey Esq. Brit. Mus.1931-291./P. coon insperatus ♀ J. and T./[red edged round label] Type/BMNH(E) # 149298 [BMNH]; 1♂, 1♀, Jianfeng Ling, Hainan, China, P. Y.-F. Lo leg. 2013–III–15 [PYFL]; 1♂, Wuzhi Shan, Hainan, China Lu Ji leg. 2014–III–12 [AMC]; 3♂, Wuzhi Shan, Hainan, China Lu Ji leg. 2014–III–15 [AMC]; 3♂, Sanya, Hainan Is., China, 2018–V [HHZ].

References

  1. Hancock, D.L. Phylogeny of the Troidine butterflies (Lepidoptera: Papilionidae) revisited: Are the red-bodied swallowtails monophyletic. Aust. Entomol. 2007, 34, 33–42. [Google Scholar]
  2. Tsukada, E.; Nishiyama, Y. Butterflies of the South East Asian Islands. Part I. Papilionidae; Plapac Co., Ltd.: Tokyo, Japan, 1980. [Google Scholar]
  3. Wallace, A.R. On the phenomena of variation and geographical distribution as illustrated by the Papilionidae of the Malayan Region. Trans. Linn. Soc. Lond. 1865, 25, 1–71, pl. 71–78. [Google Scholar] [CrossRef]
  4. Fabricius, J.C. Entomologia Systematica: Emendata et Aucta. Tom. III. Pars I; Impensis C. G. Proft, Fil. et Soc.: Hafniae, Denmark, 1793. [Google Scholar]
  5. Rothschild, W. A Revision of the Papilios of the Eastern Hemisphere, Exclusive of Africa. Novit. Zool. 1895, 2, 167–463. [Google Scholar]
  6. Moore, F. Lepidoptera Indica Vol. V. Rhopalocera. Family Nymphalidæ. Family Riodinidæ. Family Papilionidæ; Lovell Reeve: London, UK, 1902. [Google Scholar]
  7. Jordan, H.E.K. Papilio cont’d, Eurycus, Leptocircus, Teinopalpus & Armandia. In Seitz, Macrolepidoptera of the World 9, Fauna Indoaustralica; Fritz Lehmann Verlag: Stuttgart, Germany, 1909. [Google Scholar]
  8. Evans, W.H. The Identification of Indian Butterflies, 2nd ed. Revised; Bombay Natural History Society: Madras, India, 1932. [Google Scholar]
  9. Talbot, G. The Fauna of British India, Including Ceylon and Burma. Butterflies. Vol. I. (Papilionidae & Pieridae); Taylor & Francis: London, UK, 1939. [Google Scholar]
  10. Ford, E.B. Studies on the chemistry of pigments in the Lepidoptera, with reference to their bearing on systematics. 4. The classification of of the Papilionidae. Trans. R. Entomol. Soc. 1944, 94, 201–223. [Google Scholar] [CrossRef]
  11. Condamine, F.L.; Sperling, F.A.H.; Wahlberg, N.; Rasplus, J.-Y.; Kergoat, G.J. What causes latitudinal gradients in species diversity? Evolutionary processes and ecological constraints on swallowtail biodiversity. Ecol. Lett. 2012, 15, 267–277. [Google Scholar] [CrossRef]
  12. Fernando, E.; Jangid, A.K.; Chowdhury, S.; Kehimar, I.; Lo, P.; Moonen, J. Common Clubtail. IUCN Red List Threat. Species 2019. [Google Scholar] [CrossRef]
  13. Bollino, M.; Sala, G. A new species of Atrophaneura coon from Bawean Island (Indonesia) (Lepidoptera: Papilionidae). Trop. Lepid. 1992, 3, 119–122. [Google Scholar]
  14. Tytler, H.C. Notes on some new and interesting butterflies chiefly from Burma. Part I. J. Bombay Nat. Hist. Soc. 1939, 41, 235–252. [Google Scholar]
  15. Hu, S.J.; Zhang, X.; Cotton, A.M.; Ye, H. Discovery of a third species of Lamproptera Gray, 1832 (Lepidoptera: Papilionidae). Zootaxa 2014, 3786, 469–482. [Google Scholar] [CrossRef]
  16. Hu, S.J.; Cotton, A.M.; Condamine, F.L.; Duan, K.; Wang, R.J.; Hsu, Y.F.; Zhang, X.; Cao, J. Revision of Pazala Moore, 1888: The Graphium (Pazala) mandarinus (Oberthür, 1879) group, with treatments of known taxa and descriptions of new species and new subspecies (Lepidoptera: Papilionidae). Zootaxa 2018, 4441, 401–446. [Google Scholar] [CrossRef] [PubMed]
  17. Hu, S.J.; Condamine, F.L.; Monastyrskii, A.L.; Cotton, A.M. A new species of the Graphium (Pazala) mandarinus group from Central Vietnam (Lepidoptera: Papilionidae). Zootaxa 2019, 4554, 286–300. [Google Scholar] [CrossRef] [PubMed]
  18. Zhang, H.H.; Cotton, A.M.; Condamine, F.L.; Wang, R.J.; Hsu, Y.F.; Duan, K.; Zhang, X.; Hu, S.J. Revision of Pazala Moore, 1888: The Graphium (Pazala) alebion and G. (P.) tamerlanus groups, with notes on taxonomic and distribution confusions (Lepidoptera: Papilionidae). Zootaxa 2020, 4759, 77–97. [Google Scholar] [CrossRef]
  19. Ylla, J.; Peigler, R.S.; Kawahara, A.Y. Cladistic analysis of moon moths using morphology, molecules, and behaviour: Actias Leach, 1815; Argema Wallengren, 1858; Graellsia Grote, 1896 (Lepidoptera: Saturniidae). Shilap Rev. Lepidopterol. 2005, 33, 299–317. [Google Scholar]
  20. Ivshin, N.; Krutov, V.; Romanov, D. Three new taxa of the genus Cechetra Zolotuhin & Ryabov, 2012 (Lepidoptera, Sphingidae) from South-East Asia with notes on other species of the genus. Zootaxa 2018, 4450, 1–25. [Google Scholar] [PubMed]
  21. Zolotuhin, V.V. Laothoe Fabricius, 1807 (Lepidoptera: Sphingidae): How many species? Eversmannia 2018, 54, 3–12. [Google Scholar]
  22. Hebert, P.D.N.; Cywinska, A.; Ball, S.L.; de Waard, J.R. Biological identifications through DNA barcodes. Proc. R. Soc. Lond. 2003, 270, 313–321. [Google Scholar] [CrossRef]
  23. Hu, S.J.; Ning, T.; Fu, D.Y.; Haack, R.A.; Zhang, Z.; Chen, D.D.; Ma, X.Y.; Ye, H. Dispersal of the Japanese pine sawyer, Monochamus alternatus (Coleoptera: Cerambycidae), in mainland China as inferred from molecular data and associations to indices of human activity. PLoS ONE 2013, 8, e57568. [Google Scholar] [CrossRef]
  24. Folmer, O.; Black, M.B.; Hoch, W.; Lutz, R.A.; Vrijehock, R.C. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol. Mar. Biol. Biotechnol. 1994, 3, 294–299. [Google Scholar]
  25. Thompson, J.D.; Higgins, D.G.; Gibson, T.J. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl. Acids Res. 1994, 22, 4673–4680. [Google Scholar] [CrossRef]
  26. Hall, T.A. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids Symp. Ser. 1999, 41, 95–98. [Google Scholar]
  27. Kumar, S.; Stecher, G.; Tamura, K. Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 2016, 33, 1870–1874. [Google Scholar] [CrossRef] [PubMed]
  28. Song, H.; Buhay, J.E.; Whiting, M.F.; Crandall, K.A. Many species in one: DNA barcoding overestimates the number of species when nuclear mitochondrial pseudogenes are coamplified. Proc. Natl. Acad. Sci. USA 2008, 105, 13486–13491. [Google Scholar] [CrossRef] [PubMed]
  29. Bertheau, C.; Schuler, H.; Krumböck, S.; Arthofer, W.; Stauffer, C. Hit or miss in phylogenetic analyses: The case of the cryptic NUMTs. Mol. Ecol. Resour. 2011, 11, 1056–1059. [Google Scholar] [CrossRef]
  30. Kimura, M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 1980, 16, 111–120. [Google Scholar] [CrossRef] [PubMed]
  31. Ronquist, F.; Teslenko, M.; Van Der Mark, P.; Ayres, D.L.; Darling, A.; Höhna, S.; Larget, B.; Liu, L.; Suchard, M.A.; Huelsenbeck, J.P. MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Syst. Biol. 2012, 61, 539–542. [Google Scholar] [CrossRef] [PubMed]
  32. Lanfear, R.; Frandsen, P.B.; Wright, A.M.; Senfeld, T.; Calcott, B. PartitionFinder 2: New methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Mol. Biol. Evol. 2017, 34, 772–773. [Google Scholar] [CrossRef]
  33. Huelsenbeck, J.P.; Larget, B.; Alfaro, M.E. Bayesian phylogenetic model selection using reversible jump Markov Chain Monte Carlo. Mol. Biol. Evol. 2004, 21, 1123–1133. [Google Scholar] [CrossRef]
  34. Xie, W.G.; Lewis, P.O.; Fan, Y.; Kuo, L.; Chen, M.H. Improving marginal likelihood estimation for Bayesian phylogenetic model selection. Syst. Biol. 2011, 60, 150–160. [Google Scholar] [CrossRef]
  35. Zhang, J.J.; Kapli, P.; Pavlidis, P.; Stamatakis, A. A general species delimitation method with applications to phylogenetic placements. Bioinformatics 2013, 29, 2869–2876. [Google Scholar] [CrossRef]
  36. Puillandre, N.; Lambert, A.; Brouillet, S.; Achaz, G. ABGD, Automatic Barcode Gap Discovery for primary species delimitation. Mol. Ecol. 2012, 21, 1864–1877. [Google Scholar] [CrossRef]
  37. Mutanen, M.; Kivelä, S.M.; Vos, R.A.; Doorenweerd, C.; Ratnasingham, S.; Hausmann, A.; Huemer, P.; Dincă, V.; van Nieukerken, E.J.; Lopez-Vaamonde, C.; et al. Species-level para- and polyphyly in DNA barcode gene trees: Strong operational bias in European Lepidoptera. Syst. Biol. 2016, 65, 1024–1040. [Google Scholar] [CrossRef]
  38. Meyer, C.P.; Paulay, G. DNA barcoding: Error rates based on comprehensive sampling. PLoS Biol. 2005, 3, e422. [Google Scholar] [CrossRef] [PubMed]
  39. Moonen, J.J.M. Notes on some Papilionidae (Lepidoptera) from Indonesia. Trans. Lepidopterol. Soc. Jpn. 1998, 49, 219–228. [Google Scholar]
  40. Condamine, F.L.; Toussaint, E.F.A.; Cotton, A.M.; Genson, G.S.; Sperling, F.A.H.; Kergoat, G.J. Fine-scale biogeographical and temporal diversification processes of peacock swallowtails (Papilio subgenus Achillides) in the Indo-Australian Archipelago. Cladistics 2013, 29, 88–111. [Google Scholar] [CrossRef]
  41. Condamine, F.L.; Toussaint, E.F.A.; Clamens, A.-L.; Genson, G.S.; Sperling, F.A.H.; Kergoat, G.J. Deciphering the evolution of birdwing butterflies 150 years after Alfred Russel Wallace. Sci. Rep. 2015, 5, 11860. [Google Scholar] [CrossRef]
  42. Beck, J.; Kitching, I.J.; Linsenmair, K.E. Wallace’s line revisited: Has vicariance or dispersal shaped the distribution of Malesian hawkmoths (Lepidoptera: Sphingidae)? Biol. J. Linn. Soc. 2006, 89, 455–468. [Google Scholar] [CrossRef]
  43. Voris, H.K. Maps of Pleistocene sea levels in Southeast Asia: Shorelines, river systems and time durations. J. Biogeogr. 2000, 27, 1153–1167. [Google Scholar] [CrossRef]
  44. Miyazaki, S.; Saito, T.; Saito, K. Notes on the butterflies of the southern part of Vietnam (1). Yadoriga 2006, 208, 2–19. [Google Scholar]
  45. Kunte, K.; Sondhi, S.; Roy, P. Butterflies of India, v. 2.53. Available online: http://www.ifoundbutterflies.org/ (accessed on 1 May 2020).
  46. Igarashi, S.; Fukuda, K. The Life Histories of Asian Butterflies; Tokai University Press: Tokyo, Japan, 1997; Volume 1. [Google Scholar]
  47. Yao, T.L. Nine new species of Thottea (Aristolochiaceae) in Peninsular Malaysia and Singapore, with two taxa in Peninsular Malaysia redefined and a taxon lectotypified. Blumea 2013, 58, 245–262. [Google Scholar] [CrossRef]
  48. Robi, A.J.; Jose, P.A.; Udayan, P.S. Thottea sasidharaniana sp. nov (Aristolochiaceae) from Peninsular India. Nord. J. Bot. 2014, 32, 11–14. [Google Scholar] [CrossRef]
  49. Pao, N.T.; Upadhaya, K. Effect of fragmentation and anthropogenic disturbances on floristic composition and structure of subtropical broad leaved humid forest in Meghalaya, Northeast India. Appl. Ecol. Environ. Res. 2017, 15, 385–407. [Google Scholar] [CrossRef]
  50. Ansari, F.; Jeong, Y.; Putri, I.A.; Kim, S. Sociopsychological aspects of butterfly souvenir purchasing behavior at Bantimurung Bulusaraung National Park in Indonesia. Sustainability 2019, 11, 1789. [Google Scholar] [CrossRef]
  51. Hansen, M.C.; Wang, L.; Song, X.P.; Tyukavina, A.; Turubanova, S.; Potapov, P.V.; Stehman, S.V. The fate of tropical forest fragments. Sci. Adv. 2020, 6, 9. [Google Scholar] [CrossRef] [PubMed]
  52. McMorrow, J.; Talip, M.A. Decline of forest area in Sabah, Malaysia: Relationship to state policies, land code and land capability. Glob. Environ. Chang.-Hum. Policy Dimens. 2001, 11, 217–230. [Google Scholar] [CrossRef]
  53. Latinne, A.; Saputro, S.; Kalengkongan, J.; Kowel, C.L.; Gaghiwu, L.; Ransaleleh, T.A.; Nangoy, M.J.; Wahyuni, I.; Kusumaningrum, T.; Safari, D.; et al. Characterizing and quantifying the wildlife trade network in Sulawesi, Indonesia. Glob. Ecol. Conserv. 2020, 21, e00887. [Google Scholar] [CrossRef]
Insects 11 00392 g001 550
Figure 1. Distribution of the yellow and red marked Losaria coon (Fabricius, 1793), with currently accepted subspecies delimitation; the blue dash line encircles the tentative distribution range of L. coon.
Figure 1. Distribution of the yellow and red marked Losaria coon (Fabricius, 1793), with currently accepted subspecies delimitation; the blue dash line encircles the tentative distribution range of L. coon.
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Insects 11 00392 g002 550
Figure 2. The Bayesian phylogenetic tree of genus Losaria (Moore, 1902) with Pharmacophagus antenor and Pachliopta polyphontes as outgroups. The branch tip labels of taxon names follow the current taxonomy, while the names in red colour blocks reflect changes proposed in this study. Black values at nodes indicate the posterior probability, and the blue values indicate the probabilities for Losaria taxa identified by the Bayesian–Poisson tree process model (bPTP).
Figure 2. The Bayesian phylogenetic tree of genus Losaria (Moore, 1902) with Pharmacophagus antenor and Pachliopta polyphontes as outgroups. The branch tip labels of taxon names follow the current taxonomy, while the names in red colour blocks reflect changes proposed in this study. Black values at nodes indicate the posterior probability, and the blue values indicate the probabilities for Losaria taxa identified by the Bayesian–Poisson tree process model (bPTP).
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Figure 3. Morphological comparison of Losaria coon and L. doubledayi A: L. coon coon, West Java, Indonesia, male ♂; B: ditto, female ♀; C: L. doubledayi doubledayi, Ranong, Thailand, male ♂; D: L. doubledayi doubledayi, Perak, Malaysia, female ♀; E: L. doubledayi doubledayi, Wang Chin, Thailand, male ♂; F: L. doubledayi insperatus, Hainan, China. Scale bar = 10 mm.
Figure 3. Morphological comparison of Losaria coon and L. doubledayi A: L. coon coon, West Java, Indonesia, male ♂; B: ditto, female ♀; C: L. doubledayi doubledayi, Ranong, Thailand, male ♂; D: L. doubledayi doubledayi, Perak, Malaysia, female ♀; E: L. doubledayi doubledayi, Wang Chin, Thailand, male ♂; F: L. doubledayi insperatus, Hainan, China. Scale bar = 10 mm.
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Figure 4. Morphological comparison of Losaria rhodifer (A: male ♂, B: female ♀), L. neptunus (C: male ♂, D: female ♀), and L. palu (E: male ♂, F: female ♀). Upper side on the first row, underside on the second row; scale bar = 10 mm.
Figure 4. Morphological comparison of Losaria rhodifer (A: male ♂, B: female ♀), L. neptunus (C: male ♂, D: female ♀), and L. palu (E: male ♂, F: female ♀). Upper side on the first row, underside on the second row; scale bar = 10 mm.
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Figure 5. Comparison of male genitalia of Losaria coon (A) and L. doubledayi (B); scale bars = 1.0 mm.
Figure 5. Comparison of male genitalia of Losaria coon (A) and L. doubledayi (B); scale bars = 1.0 mm.
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Figure 6. Comparison of female genitalia of Losaria coon coon (A) and L. doubledayi (B), scale bars = 1.0 mm; with enlarged signum (C) and (D), scale bars = 0.5 mm.
Figure 6. Comparison of female genitalia of Losaria coon coon (A) and L. doubledayi (B), scale bars = 1.0 mm; with enlarged signum (C) and (D), scale bars = 0.5 mm.
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Insects 11 00392 g007 550
Figure 7. Male genitalia of Losaria rhodifer (A), L. neptunus (B), and L. palu (C); scale bars = 1.0 mm.
Figure 7. Male genitalia of Losaria rhodifer (A), L. neptunus (B), and L. palu (C); scale bars = 1.0 mm.
Insects 11 00392 g007
Insects 11 00392 g008 550
Figure 8. Female genitalia of Losaria rhodifer (A), L. neptunus (B), and L. palu (C), scale bars = 1.0 mm; with enlarged signum (D–F), scale bars = 0.5 mm.
Figure 8. Female genitalia of Losaria rhodifer (A), L. neptunus (B), and L. palu (C), scale bars = 1.0 mm; with enlarged signum (D–F), scale bars = 0.5 mm.
Insects 11 00392 g008
Table
Table 1. Sampling information and GenBank/BOLD accession numbers of the Losaria species and outgroups used in this study. The taxon names follow current taxonomy, mentioned above.
Table 1. Sampling information and GenBank/BOLD accession numbers of the Losaria species and outgroups used in this study. The taxon names follow current taxonomy, mentioned above.
Taxon (Sample Code)LocalityCollecting DateAccession No.
Losaria coon coon (LCN002)West Java, Indonesia2018-IMT417883
Losaria coon coon (LCN011)West Java, Indonesia2018-IMT417884
Losaria coon coon (LCN012)West Java, Indonesia2018-IMT417883
Losaria coon coon (LCN014)West Java, Indonesia2018-IMT417883
Losaria coon sangkapurae (LCN015)Bawean Is., Indonesia2018-IIMT417885
Losaria coon sangkapurae (LCN016)Bawean Is., Indonesia2018-IIMT417885
Losaria coon doubledayi (MYENT161-17)Lenya, Tanintharyi, Myanmar2015-V-29MYENT161-17
Losaria coon doubledayi (KHCBT552-11)Khao Chong, Trang, Thailand2010-XII-26KHCBT552-11
Losaria coon doubledayi (LCN005)Ranong, Thailand2018-IIMT417886
Losaria coon doubledayi (LCN009)Ranong, Thailand2018-IIMT417886
Losaria coon doubledayi (LCN018)Dong Tien, Binh Thuan, Vietnam2018-VIIMT417886
Losaria coon doubledayi (LCN019)Thac Mai, Dong Nai, Vietnam2018-VIIIMT417886
Losaria coon doubledayi (LCN133)Wang Chin, Phrae, Thailand2017-XI-11MT417888
Losaria coon doubledayi (LCN134)Wang Chin, Phrae, Thailand2017-XI-11MT417888
Losaria coon doubledayi (LCN135)Wang Chin, Phrae, Thailand2017-XI-11MT417888
Losaria coon insperatus (LCN128)Sanya, Hainan Is., China2018-VMT417887
Losaria coon insperatus (LCN129)Sanya, Hainan Is., China2018-VMT417887
Losaria coon insperatus (LCN130)Sanya, Hainan Is., China2018-VMT417887
Losaria rhodifer (LRH04)Andaman Is., India2014-XMT417889
Losaria rhodifer (LRH05)Andaman Is., India1962-IIIMT417890
Losaria neptunus neptunus (KHCBT1271-16)Khao Chong, Trang, Thailand2014-V-2KHCBT1271-16
Losaria neptunus doris (LNE01)West Kalimantan, Indonesia2018-IMT417891
Losaria neptunus creber (LNE02)Simeulue Is., Indonesia2010-VIIIMT417892
Losaria neptunus creber (LNE04)Simeulue Is., Indonesia2010-VIIIMT417893
Losaria neptunus creber (LNE05)Simeulue Is., Indonesia2010-VIIIMT417894
Losaria palu (LPA01)Donggala, Sulawesi, Indonesia2017-XMT417895
Losaria palu (LPA02)Donggala, Sulawesi, Indonesia2018-IIMT417895
Losaria palu (LPA03)Donggala, Sulawesi, Indonesia2018-IIMT417895
Pachliopta polyphontes (PAC01)Muna Is., Indonesia2017-XIIMT417896
Pharmacophagus antenor (PHA01)Madagascar2016-VIMT417897
Table
Table 2. Monophylizer assessment of the Losaria species and subspecies used in this study.
Table 2. Monophylizer assessment of the Losaria species and subspecies used in this study.
TaxonAssessmentTanglees
1. L. coonmonophyletic—
1a. L. coon coonparaphyleticL. coon sangkapurae
1b. L. coon sangkapuraemonophyletic—
2. L. doubledayimonophyletic—
2a. L. doubledayi doubledayi 1paraphyleticL. doubledayi doubledayi 2;L. doubledayi insperatus
2b. L. doubledayi doubledayi 2paraphyleticL. doubledayi insperatus
2c. L. doubledayi insperatusmonophyletic—
3. L. rhodifermonophyletic—
4. L. neptunusmonophyletic—
5. L. palumonophyletic
Table
Table 3. The Kimura two-parameter distances (in percentages) between all taxa of genus Losaria (Moore, 1902), with species and subspecies identified as in the Bayesian phylogenetic tree, as shown in Figure 2.
Table 3. The Kimura two-parameter distances (in percentages) between all taxa of genus Losaria (Moore, 1902), with species and subspecies identified as in the Bayesian phylogenetic tree, as shown in Figure 2.
1a1b2a2b2c345
1a. L. coon coon
1b. L. coon sangkapurae0.19
2a. L. doubledayi doubledayi 13.083.28
2b. L. doubledayi doubledayi 22.442.640.61
2c. L. doubledayi insperatus3.573.770.461.08
3. L. rhodifer3.173.353.353.003.35
4. L. neptunus6.116.326.766.327.036.47
5. L. palu7.838.048.928.409.289.615.19
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