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Open AccessArticle

Rapid and Simple Species Identification of Cicada Exuviae Using COI-Based SCAR Assay

Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Naju 58245, Korea
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Insects 2020, 11(3), 168; https://doi.org/10.3390/insects11030168
Received: 5 February 2020 / Revised: 27 February 2020 / Accepted: 4 March 2020 / Published: 6 March 2020
Cicadidae periostracum (CP), the medicinal name of cicada exuviae, is well-known insect-derived traditional medicine with various pharmacological effects, e.g., anticonvulsive, anti-inflammatory, antitussive, and anticancer effects; it is also beneficial for the treatment of Parkinson’s disease. For appropriate CP application, accurate species identification is essential. The Korean pharmacopoeia and the pharmacopoeia of the People’s Republic of China define Cryptotympana atrata as the only authentic source of CP. Species identification of commercially distributed CP based on morphological features, however, is difficult because of the combined packaging of many cicada exuviae in markets, damage during distribution, and processing into powder form. DNA-based molecular markers are an excellent alternative to morphological detection. In this study, the mitochondrial cytochrome c oxidase subunit I sequences of C. atrata, Meimuna opalifera, Platypleura kaempferi, and Hyalessa maculaticollis were analyzed. On the basis of sequence alignments, we developed sequence-characterized amplified-region (SCAR) markers for efficient species identification. These markers successfully discriminated C. atrata from the three other cicada species, and detected the adulteration of market CP samples. This SCAR assay is a rapid, simple, cheap, reliable, and reproducible method for species identification, regardless of sample form and status, and contributes to CP quality control. View Full-Text
Keywords: cicadidae periostracum; Cryptotympana atrata; cytochrome C oxidase subunit I; molecular authentication; sequence-characterized amplified region cicadidae periostracum; Cryptotympana atrata; cytochrome C oxidase subunit I; molecular authentication; sequence-characterized amplified region
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Noh, P.; Kim, W.J.; Song, J.-H.; Park, I.; Choi, G.; Moon, B.C. Rapid and Simple Species Identification of Cicada Exuviae Using COI-Based SCAR Assay. Insects 2020, 11, 168.

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