Physiological Analysis and Transcriptome Analysis of Asian Honey Bee (Apis cerana cerana) in Response to Sublethal Neonicotinoid Imidacloprid
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Insects Material
2.2. Pesticide Exposure Experiment
2.3. Climbing Assays
2.4. Sucrose Responsiveness
2.5. Enzyme Activity Assay
2.6. RNA Isolation and RNA Sequencing Analysis
2.7. Bioinformatics Analysis of RNA-seq Data
2.8. Confirmation of RNA-Seq Results by qRT- PCR
2.9. Statistical Analyses
3. Results
3.1. Climbing Ability, and Sucrose Responsiveness Changes of A. cerana after Exposed to Imidacloprid
3.2. Influences of Imidacloprid on Detoxification Enzyme Activities
3.3. Illumina Sequencing and Transcriptome Assembly
3.4. DEGs in Response to Sublethal Concentration of Imidacloprid
3.5. Trend Analysis of DEGs
3.6. Functional Annotation and Classification of Differentially Expressed Genes
3.7. Validation of RNA-seq Data by Quantitative Real-Time PCR
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Gene Name | Pathway | Gene Description | Profile |
---|---|---|---|
LOC108002218 | AMP | abaecin | 12 |
LOC108000468 | AMP | apidaecins type 22 | 12 |
LOC107993803 | AMP | defensin-1 | 12 |
LOC108000415 | AMP | defensin-2 | 12 |
LOC107993492 | AMP | hymenoptaecin | 12 |
LOC108001747 | AMP | hymenoptaecin pseudogene | 12 |
LOC108004481 | Jak/STAT | E3 ubiquitin-protein ligase TRIM71-like | 17 |
LOC108000724 | Jak/STAT | E3 ubiquitin-protein ligase MARCH2-like | 1 |
LOC108003398 | Ribosomal Biosynthesis | 60S ribosomal protein L35 | 1 |
LOC107992528 | Ribosomal Biosynthesis | 60S ribosomal protein L37a | 1 |
LOC108000385 | Ribosomal Biosynthesis | 60S ribosomal protein L39 | 2 |
LOC108001255 | Ribosomal Biosynthesis | 60S ribosomal protein L28-like | 2 |
LOC107994696 | Ribosomal Biosynthesis | ribosomal RNA processing protein 1 homolog | 7 |
LOC108002849 | Ribosomal Biosynthesis | 60S ribosomal protein L37 | 8 |
LOC108003508 | Ribosomal Biosynthesis | histone H2A-like | 1 |
LOC107998816 | Ribosomal Biosynthesis | histone H2A.Z-specific chaperone CHZ1-like | 7 |
LOC107996866 | Toll/TLR | toll-like receptor 4 | 7 |
LOC107998162 | Toll/TLR | toll-like receptor 13 | 17 |
LOC108001172 | Toll/TLR | protein toll-like | 11 |
LOC107995659 | Toll/TLR | protein toll | 12 |
LOC107996104 | Toll/TLR | NF-kappa-B inhibitor cactus-like | 16 |
LOC108002391 | Toll/TLR | NF-kappa-B inhibitor cactus-like | 16 |
LOC107998380 | Toll/TLR | serine protease inhibitor 3-like | 10 |
LOC107994873 | Toll/TLR | serine protease gd-like | 12 |
LOC108002098 | Toll/TLR | serine protease snake-like | 12 |
LOC108004289 | Toll/TLR | serine/threonine-protein kinase 32B | 12 |
LOC107999607 | Toll/TLR | serine/threonine-protein kinase PLK1-like | 16 |
LOC108003108 | Toll/TLR | serine/threonine-protein kinase Nek5-like | 17 |
LOC107996922 | IG Superfamily Genes | lachesin-like | 12 |
LOC107995322 | leukocyte | leukocyte elastase inhibitor-like | 12 |
LOC107996108 | Lysozyme | lysosomal alpha-mannosidase | 9 |
LOC107999764 | Scav. Receptor B | scavenger receptor class B member 1 | 1 |
Gene Name | Gene Description | Profile |
---|---|---|
LOC107996029 | odorant-binding protein 59a-like | 1 |
LOC108002840 | general odorant-binding protein 69a-like (ASP1) | 4 |
LOC107999620 | general odorant-binding protein 56d-like (OBP13) | 9 |
LOC107996745 | odorant receptor 4-like | 10 |
LOC107996764 | odorant receptor 4-like | 12 |
LOC107996743 | odorant receptor 4-like | 14 |
LOC107996763 | odorant receptor 4-like | 15 |
LOC107996720 | odorant receptor 4-like | 7 |
LOC108000540 | odorant receptor 13a-like | 5 |
LOC107994426 | odorant receptor 13a-like | 17 |
LOC107996760 | odorant receptor 22c-like | 10 |
LOC107999581 | odorant receptor 63a-like | 15 |
LOC107996752 | odorant receptor 49b-like | 17 |
LOC108002046 | glutamate receptor ionotropic, kainate 2 | 17 |
LOC108000903 | glutamate receptor ionotropic, NMDA 2C-like | 3 |
LOC107997799 | gustatory receptor for sugar taste 64f-like | 10 |
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Gao, J.; Jin, S.-S.; He, Y.; Luo, J.-H.; Xu, C.-Q.; Wu, Y.-Y.; Hou, C.-S.; Wang, Q.; Diao, Q.-Y. Physiological Analysis and Transcriptome Analysis of Asian Honey Bee (Apis cerana cerana) in Response to Sublethal Neonicotinoid Imidacloprid. Insects 2020, 11, 753. https://doi.org/10.3390/insects11110753
Gao J, Jin S-S, He Y, Luo J-H, Xu C-Q, Wu Y-Y, Hou C-S, Wang Q, Diao Q-Y. Physiological Analysis and Transcriptome Analysis of Asian Honey Bee (Apis cerana cerana) in Response to Sublethal Neonicotinoid Imidacloprid. Insects. 2020; 11(11):753. https://doi.org/10.3390/insects11110753
Chicago/Turabian StyleGao, Jing, San-Sheng Jin, Yan He, Jin-Hong Luo, Chun-Qin Xu, Yan-Yan Wu, Chun-Shen Hou, Qiang Wang, and Qing-Yun Diao. 2020. "Physiological Analysis and Transcriptome Analysis of Asian Honey Bee (Apis cerana cerana) in Response to Sublethal Neonicotinoid Imidacloprid" Insects 11, no. 11: 753. https://doi.org/10.3390/insects11110753