A Strain of Bacillus thuringiensis Containing a Novel cry7Aa2 Gene that Is Toxic to Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strains, Plasmids and Insect Culture Conditions
2.2. Total DNA Extraction and Genomic Sequencing
2.3. Identification of Potential Insecticidal Genes
2.4. Amplification and Cloning of the cry7Aa2 Gene
2.5. Production of Spores and Crystals from Wild and Recombinant Bt Strains
2.6. Analysis of Crystal Proteins
2.7. Leptinotarsa Decemlineata Bioassays
2.8. Nucleotide Sequence Accession Number
3. Results
3.1. Draft Genome Sequence of the Bacillus thuringiensis BM311.1 Strain
3.2. Characterization of Cry7Aa2
3.3. Insecticidal Activity of Cry7Aa2 for L. decemlineata
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Target Database | Identity (%) | MW (KDa) | Length (Nº Residues) | Predicted Location |
---|---|---|---|---|
Cry7Aa1 | 98 | 129 | 1138 | Plasmid |
Cry60Aa1 | 18 | 35 | 322 | Chromosome |
Cry60Aa3 | 19 | 33 | 303 | Chromosome |
Mtx-like | 94 | 57 | 515 | Plasmid |
Bacillolysin | 99 | 61 | 556 | Chromosome |
Bacillolysin | 96 | 98 | 893 | Plasmid |
Peptidase M4 | 99 | 65 | 583 | Chromosome |
Peptidase M4 | 99 | 62 | 567 | Unclassified |
Peptidase M4 | 99 | 62 | 552 | Plasmid |
Peptidase M4 | 99 | 61 | 566 | Chromosome |
Bt Strains/Protein | Regression Lines | LC50 (µg/mL) | Goodness of Fit | Relative Potency (b) | Fiducial Limits (95%) | |||
---|---|---|---|---|---|---|---|---|
Slope ± SE | Intercept ± SE | χ2 | df (a) | Lower | Upper | |||
BM311.1 | 0.63 ± 0.10 | 4.19 ± 0.13 | 18.89 | 0.99 | 3 | 1 | ||
BMB171-Cry7Aa2 | 1.16 ± 0.19 | 3.46 ± 0.29 | 20.80 | 1.18 | 3 | 0.91 | 0.39 | 2.13 |
BMB171-Cry7Aa2-TA (c) | 1.99 ± 0.54 | 3.61 ± 0.53 | 4.93 | 1.02 | 2 | 3.83 | 1.57 | 9.33 |
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Domínguez-Arrizabalaga, M.; Villanueva, M.; Fernandez, A.B.; Caballero, P. A Strain of Bacillus thuringiensis Containing a Novel cry7Aa2 Gene that Is Toxic to Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae). Insects 2019, 10, 259. https://doi.org/10.3390/insects10090259
Domínguez-Arrizabalaga M, Villanueva M, Fernandez AB, Caballero P. A Strain of Bacillus thuringiensis Containing a Novel cry7Aa2 Gene that Is Toxic to Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae). Insects. 2019; 10(9):259. https://doi.org/10.3390/insects10090259
Chicago/Turabian StyleDomínguez-Arrizabalaga, Mikel, Maite Villanueva, Ana Beatriz Fernandez, and Primitivo Caballero. 2019. "A Strain of Bacillus thuringiensis Containing a Novel cry7Aa2 Gene that Is Toxic to Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)" Insects 10, no. 9: 259. https://doi.org/10.3390/insects10090259