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Open AccessArticle

High-Resolution Profiling of Gut Bacterial Communities in an Invasive Beetle using PacBio SMRT Sequencing System

by Letian Xu 1,†, Liuwei Sun 1,†, Shihan Zhang 2,†, Shanshan Wang 3 and Min Lu 4,*
1
State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China
2
Cardiff Sixth Form College, Cardiff CF24 0AA, UK
3
Institute of Physical Science and Information Technology, Anhui University, Hefei 230601, China
4
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to the study.
Insects 2019, 10(8), 248; https://doi.org/10.3390/insects10080248
Received: 12 July 2019 / Revised: 3 August 2019 / Accepted: 11 August 2019 / Published: 14 August 2019
(This article belongs to the Special Issue Ecology and Management of Invasive Insects in Forest Ecosystems)
Dendroctonus valens, an invasive bark beetle, has caused severe damage to Chinese forests. Previous studies have highlighted the importance of the gut microbiota and its fundamental role in host fitness. Culture-dependent and culture-independent methods have been applied in analyzing beetles’ gut microbiota. The former method cannot present a whole picture of the community, and the latter mostly generates short read lengths that cannot be assigned to species. Here, the PacBio sequencing system was utilized to capture full-length 16S rRNA sequences in D. valens gut throughout its ontogeny. A total of eight phyla, 55 families, 102 genera, and 253 species were identified. Bacterial communities in colonized beetles have the greatest richness but the lowest evenness in all life stages, which is different from those in young larvae. Pseudomonas sp., Serratia liquefaciens possess high abundance throughout its ontogeny and may serve as members of the core bacteriome. A phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) analysis predicted that gut microbiota in larvae are rich in genes involved in carbohydrate, energy metabolism. Gut microbiota in both larvae and colonized beetles are rich in xenobiotics and terpenoids biodegradation, which are decreased in dispersal beetles. Considering that the results are based mainly on the analysis of 16S rRNA sequencing and PICRUSt prediction, further confirmation is needed to improve the knowledge of the gut microbiota in D. valens and help to resolve taxonomic uncertainty at the species level. View Full-Text
Keywords: Dendroctonus valens; bark beetle; gut bacteria; PacBio; high-resolution profiling; ontogeny Dendroctonus valens; bark beetle; gut bacteria; PacBio; high-resolution profiling; ontogeny
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Xu, L.; Sun, L.; Zhang, S.; Wang, S.; Lu, M. High-Resolution Profiling of Gut Bacterial Communities in an Invasive Beetle using PacBio SMRT Sequencing System. Insects 2019, 10, 248.

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