Next Article in Journal
Changing Trends in Surgical Management of Nephrolithiasis among Young Adults: A 15-Year Population-Based Study
Previous Article in Journal
Lymphatic Tissue Transfer: Ultrasound-Guided Description and Preoperative Planning of Vascularised Lymph Nodes, Lymphatic Units, and Lymphatic Vessels Transfers
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
JPM
Volume 12
Issue 8
10.3390/jpm12081347
jpm-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Review

Endotyping of Cholesteatoma: Which Molecular Biomarkers? A Systematic Review

by
Elena Cantone
1,2,*,
Claudio Di Nola
1,2,
Eugenio De Corso
3,
Michele Cavaliere
1,2,
Giusi Grimaldi
1,2,
Anna Rita Fetoni
4,5,† and
Gaetano Motta
6,†
1
Department of Neuroscience, Reproductive and Odontostomatological Sciences-ENT Section, University of Naples Federico II, 80131 Naples, Italy
2
Head and Neck Department-ENT Section, AOU Federico II, 80131 Naples, Italy
3
Head and Neck Surgery-Otorhinolaryngology, Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, Italy
4
Department of Neuroscience, Reproductive and Odontostomatological Sciences-Audiology Section, University of Naples Federico II, 80131 Naples, Italy
5
Head and Neck Department-Audiology Section, AOU Federico II, 80131 Naples, Italy
6
Otorhinolaryngology, Head and Neck Surgery Unit, Department of Mental and Physical Health and Preventive Medicine, Università degli Studi della Campania Luigi Vanvitelli, 80131 Naples, Italy
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
J. Pers. Med. 2022, 12(8), 1347; https://doi.org/10.3390/jpm12081347
Submission received: 11 August 2022 / Revised: 18 August 2022 / Accepted: 20 August 2022 / Published: 21 August 2022
(This article belongs to the Section Clinical Medicine, Cell, and Organism Physiology)
Browse Figure
Review Reports Versions Notes

Abstract

:
Background: So far, no medical treatment is available for cholesteatoma (C) and the only effective therapy is complete surgical removal, but recurrence is common even after surgical treatment. While C is classically divided into two clinical phenotypes, congenital and acquired, only a few studies have focused on its potential biomarkers. This study aims to revise the literature to identify which biomarkers can define the endotype of C. Methods: We conducted a systematic review in accordance with the Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) process to identify published experimental articles about molecular biomarkers in C. Results: KGF and its receptor, MMP-9, KRT-1, KRT-10, and MIF might be considered biomarkers of recurrence, whereas Ki-67, TLR-4, RANKL, IL17, MMP-2, MMP-9, IL6, TNF-α, should be considered more specifically as biomarkers of bony erosion. Conclusions: These results are interesting especially from a prognostic point of view, nevertheless more studies are needed to search new biomarkers of C that could completely change not only the therapeutic standards of the disease, but also the clinical history of C itself in the era of precision medicine.

1. Introduction

C is a chronic middle ear (ME) disease, that can be defined as the accumulation of desquamating keratinized squamous epithelium within the ME accompanied by chronic inflammation [1]. C is considered a benign lesion with a gradual and destructive expansion. The proliferation of the epithelium and the erosion of the surrounding bone can lead to severe complications such as ossicular chain destruction, hearing loss, vestibular dysfunction, facial paralysis, and secondary infections as meningitis, brain abscesses, septic cavernous sinus thrombosis [2,3,4]. So far, no medical treatment is available for C and the only effective therapy is complete surgical removal, but recurrence is common even after surgical treatment. However, the reported relapse rate in the middle ear cholesteatoma (MEC) shows a wide range in the literature, from 0 to 72.4% and reflects patient’s age, surgical approaches, and minimum follow-up [5].
From a clinical point of view, C is a complex and heterogeneous disease often associated with chronic otitis media (COM) or some rare congenital syndromes affecting ear morphology and related pathologies [6]. For instance, the clinical expression of C may vary greatly. In terms of etiology, C has been mainly classified into two types: acquired and congenital C. Congenital cholesteatoma (CC) is described as a cystic mass with keratinizing squamous epithelium in the tympanic cavity or mastoid with an unbroken tympanic membrane (TM) and without a history of chronic otitis media (COM) or ME surgery [7]. CC is further classified into the closed-type, characterized by epithelial cysts without keratin exposure, and the open-type, characterized by ruptured cyst and proliferation of flat keratinizing epithelium. Whilst acquired C (AC) is classified into the pars tensa type and pars flaccida type based on its location [8]. AC is thought to be caused by the retraction of the TM, whereas CC seems to derive from an ectopic epidermoid at rest or ingrowth of meatal epidermis [9,10,11,12].
Furthermore, authors tried to differentiate subtypes/phenotypes of C basing on index of severity such as age of onset, uni- or bi-lateral disease, signs of local aggressiveness, and relapsing, missing a possible link with different pathophysiological mechanisms underlying the clinical manifestations because endotypes are not yet known. For the same reason, it is difficult to predict the clinical behavior of C, which may vary from a slow-progressing benign diseases to more aggressive disease [13]. Consequently, determining an appropriate follow-up period duration for patients with C is critical, and good prognostic biomarkers are needed in selecting patients who will require immediate surgical intervention [8].
Resuming literature data suggest that C is a heterogeneous condition in clinical presentations (phenotype) that may be related to several unknown underlying pathobiological mechanisms (endotype). In general, the phenotype defines any clinical observable features of a disease, without establishing a direct etiologic relationship with a distinct pathophysiologic mechanism, whereas the endotype describes different subgroups that share the same pathophysiologic processes leading to the development, the progression, and the presentation of a disease. Currently, only a few studies are available in the literature about biomarkers of C, some of which are mainly related to its etiopathogenesis and others to its clinical features as recurrence disease and bony erosion [2,14,15,16,17,18,19,20,21,22].
So far, no reviews have identified biomarkers of C endotyping. This study aims to revise the literature data in order to identify biomarkers defining the underlying pathobiology of the disease.

2. Materials and Methods

We conducted a systematic review in accordance with the PRISMA process [23] to identify published experimental and clinical articles about molecular biomarkers in C (Figure 1). Manuscripts were screened primarily by Ovid Medline and EMBASE and from other sources (Pubmed Central, Cochrane review, Web of Science, and Google Scholar) and published from January 2001 to June 2022. Literature searches were performed in July 2022. The authors focused on experimental studies matching the term as follow: [(cholesteatoma) OR (cholesteatomaous otitis media) OR (middle ear cholesteatoma) OR (chronic suppurative otitis media with cholesteatoma)] AND [(biomarker) OR (marker) OR (phenotype) OR (biochemistry) OR (signaling) OR (recurrence) OR (recidivism) OR (bone erosion) OR (bone destruction) OR (bone resorption) OR (complications)].
In the first screening, the authors read the title and abstract of articles selecting those being as inclusive as possible. The abstracts were screened independently by reviewers of the two groups. Inclusion and exclusion criteria were established before the selection of relevant studies. The inclusion criteria were primary research studies (including descriptive studies, observational studies, randomized trials, and basic science articles), published after January 2001. We excluded secondary research studies (e.g., review articles or systematic review), case studies, newspaper article, lecture, letter, comment, personal narrative, consensus conference, editorial. Only articles with full text available were included. Additional studies were manually identified from the reference lists of retrieved literature. We excluded all the articles that did not meet the inclusion criteria or deal directly with the issue investigated. We included only English-language peer-reviewed papers.

3. Results and Discussion

Our search yielded 2932 articles after duplicates removal. We excluded 1313 articles due to time of publication and type of article, and then 1619 were finally screened. This resulted in 9 publications which identified 13 molecular biomarkers. The full texts were assessed. We summarized in Table 1 the included studies. A biomarker has been defined as an objectively measured parameter that serves as an indicator of normal biological processes, pathogenic processes, or pharmacologic responses to a therapeutic intervention [24]. Recent literature has focused research on some biomarkers of C severity (in terms of recurrence and bone resorption).
Literature on C showed some biomarkers associated with bone resorption and other biomarkers associated with C recurrence (Table 2 and Table 3).
Most studies analyzed C specimens through immunohistochemistry and in situ hybridation, comparing results to normal retroauricular skin [14,17,18,20,21,22]. One study analyzed the expression of cytokeratins, TP53 and Matrix Metalloproteinasis-9 (MMP9) through quantitative real-time PCR [16]. Only one study evaluated serum level of MMP-2, MMP-9, and IL-6 in patients with COM [19].
Keratinocyte growth factor (KGF) is a member of fibroblast growth factor family involved in proliferation of epithelial cells and in wound healing. KGF and its receptor KGF-R play an important role in C pathogenesis [14]. Yamamoto-Fukuda investigated the possible involvement of KGF and KGF-R in the pathogenesis of C using in situ hybridization and immunohistochemistry, respectively. The results of this study indicated the possible involvement of both KGF and KGF-R in enhanced epithelial cell proliferative activity and recurrence of C [14].
In the same study, Yamamoto et al. evaluated the assessment of the proliferative activity of C using the labeling index for Ki-67. Ki-67 is a nuclear protein related to cellular proliferation and it is commonly used in mitotic index and tumor grading [15]. The authors showed a significantly higher Ki-67 labeling index in KGF/KGF-R cases than other cases [14]. According to Araz-Server et al., although the recurrent and non-recurrent C did not show significant differences in terms of the percentages of stained cells for either Ki-67 or pronuclear cell antigen (PCNA), the authors detected high Ki-67 staining in the malleus involvement group. They concluded that cell-proliferation markers could not be defined as indicators of recurrence of C, but they could be defined as indicators of destructive patterns of this disease [15].
Cytokeratins are proteins expressed in epithelial cells frequently used as biomarkers of cell migration, differentiation, and proliferation. Interactions between cytokeratins and other proteins can modulate signaling cascades involved in cell migration, invasion, and metastasis. It seems that their expression pattern correlates with the prognosis and metastatic potential of breast cancer and tumor of oral cavity [16]. Palko et al. investigated the expression of three cytokeratin genes (KRT1, KRT10 and KRT19), the matrix metalloproteinase 9 gene (MMP9) and the tumor suppressor TP53 gene in surgical samples of pediatric and adult C. They found that the expression of KRT1 and KRT10 was higher in pediatric recurrent C than in adults, whereas the expression of KRT19 was lower in more invasive recurrent C, both in pediatric and adult subjects. They also found significantly elevated expression of MMP9 in recurrent C in adults [16]. Toll-like receptors (TLR) are the first line mucosal defense, initiating the innate immune response their activation and subsequent inflammatory cytokine production play a key role in the osteoclast formation [17].
Si et al. [17] found a correlation between TLR4 expression in human acquired C and disease severity, invasion, bone destruction, and hearing loss [17]. Osteoclasts seem to modulate bone erosion in C. The differentiation and activation of osteoclasts are regulated by macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear factor κB ligand (RANKL) [25]. Overproduction of RANKL is implicated in a variety of degenerative bone diseases, such as rheumatoid arthritis (RA) and periodontitis. In RA, osteoclasts are responsible for bone erosion and undergo differentiation and activation by RANKL [26]. In the study of Imai et al., the authors found a significantly larger number of osteoclasts in the eroded bone adjacent to C than in unaffected areas as well as peri-matrix fibroblasts expression of RANKL. They also showed increased concentration of interleukin (IL)-1β, IL6, tumor necrosis factor α (TNF α), and prostaglandin E2 in C. The presence of inflammatory cells in the C peri-matrix emphasizes the role of upregulation of factors related to osteoclast activation [18]. Accordingly, with these data in our experience increased expression (IL)-1β in specimens collected by patients affected by acquired C as compared to those with congenital C support the hypothesis of proinflammatory pathway activation in more symptomatic C (unpublished data).
IL17, that has a role in pathogenesis of RA, induces the production of IL1, TNF-α and IL6 by synovial fibroblast, monocytes and macrophages and the up-regulation of matrix MMPO, nitric oxide and RANKL in chondrocytes and osteoblasts, leading to inflammatory bone destruction [27]. It seems that IL17 may induce bone destruction in patients with C through underlying mechanism of RA [20].
IL17 is also involved in type-3 immune response of CRS, directed against extracellular bacteria and fungi, where neutrophil activity may play a role in barrier damage [28].
Artono et al. [21] demonstrated a positive correlation between IL1a and TNF-a, and the severity of bone destruction in C patients.
Macrophage migration inhibitory factor (MIF) serves as a regulator of innate and acquired immunities acting as a proinflammatory agent at sites of inflammation. MIF confers antimicrobial activity to macrophages against bacterial and viral infections.
According to Choufani [22], MIF expression in human C is related to the presence of relapses. While previous research pointed out the role of different biomarkers in the C tissue, only Wu et al. analyzed the expression level of MMP-2, MMP-9, and IL6 in the serum of C patients [19]. This strategy may help to find easily accessible markers to use in clinical practice. MMP-9 is a protease involved in the degradation of extracellular matrix and basement membrane [29].
They found high expression levels of MMP-2, MMP-9 and IL-6 in the serum of C patients positively correlated with the injury degree of ossicle, which may be a sign of poor prognosis of C [19].
According to Wang et al. and in line with the united airway hypothesis, MMP-9 gene polymorphisms may influence susceptibility to the development of CRSwNP [30]. In addition, the expression levels of MMP-2, MMP-9 and IL-6 are closely related to the clinical manifestations and the severity of diabetic diseases [31]. In a more recent study, Yamamoto-Fukuda et al. also demonstrated that the use of an inhibitor of the complex Menin-MLL (MI-503) blocked the progression of middle ear C in vivo model [32].
Based on the recent literature on C biomarkers, KGF and its receptor, MMP-9, KRT-1, KRT-10, and MIF might be considered biomarkers of recidivism, whereas Ki-67, TLR-4, RANKL, IL17, MMP-2, MMP-9, IL6, TNF-α, and IL-1α might be biomarkers of bony erosion. MMP-9 could be a valid molecular biomarker of both recidivism and bony erosion. Furthermore, while MMP-9, KRT-1, KRT-10, MIF, Ki-67, TLR-4, RANKL, IL17, TNF-α, and IL-1α have been studied in tissue specimen, MMP-2, MMP-9, and IL6 have been found in serum.
The expression of biomarkers may vary with age (adults vs. children), duration of the disease and type of sample. Unfortunately, we found only one paper in the literature analyzing biomarkers in adults vs. children [16]. Indeed, in the study by Palkò et al. the authors found increased expression of KRT1 and KRT10, in pediatric cases versus adults, especially in pediatric recurrent samples [16]. Conversely, the expression of KRT19 was lower in invasive recurrent cases in both the pediatric group and groups of adults. Furthermore, significantly elevated tissue MMP9 expression was found in recurrent cases in adults. This study demonstrated that the expression of cytokeratin distinguishes between pediatric/adult, non-recurrent/recurrent cases, suggesting that distinct differentiation state and cell division potential characterize them cases of C [16]. Likely, there is only one study in the literature measuring serum biomarkers, but no studies compare the expression of tissue biomarkers with those of serum [19].
Measuring serum markers is certainly desirable because it would make the study of endotyping more accessible and less invasive.
However, future studies are needed to increase our knowledge of specific non-invasive diagnostic and prognostic tools. In addition, it would be very interesting to evaluate the level of biomarkers in different cohorts (adults/children), in different expressions of the C (recurrent/non-recurrent) and in different samples (tissue/serum) and, eventually, according to the duration of the C.
Furthermore, precise identification of specific biomarkers of aggressiveness could lead to a more personalized management (i.e., timing of intervention, recurrence prevention) and to a future identification of anti-growth/anti-proliferative agents as non-surgery therapeutic option.

4. Conclusions

Recent basic science research has led to significant advances in understanding the pathophysiological processes of disease. These processes define the endotype of a disease opening the way for the development of new diagnostic tools and innovative targeted treatments, especially in the rhinologic fields [33,34,35,36,37,38]. As has already happened for asthma and CRSwNP, and for many other autoimmune and oncological pathologies, the study of molecular biomarkers might help to develop new potential therapeutic target, and to improve an early diagnosis and a more accurate prognosis. In addition, molecular biomarkers might be used for a better scheduled follow-up.
Our data suggest that KGF and its receptor, MMP-9, KRT-1, KRT-10, and MIF might be considered biomarkers of recurrence, whereas Ki-67, TLR-4, RANKL, IL17, MMP-2, MMP-9, IL6, TNF-α, should be considered more specifically as biomarkers of bony erosion. These results are interesting, especially from a prognostic point of view; nevertheless, more studies are needed to search new biomarkers of C that could completely change not only the therapeutic standards of the disease, but also the clinical history of C itself.

Author Contributions

Conceptualization, E.C. and E.D.C.; methodology, E.C., E.D.C. and C.D.N.; validation, E.C., E.D.C., A.R.F. and G.M.; formal analysis, E.C. and G.G.; investigation, E.C. and C.D.N.; resources, M.C.; data curation, G.G. and M.C.; writing—original draft preparation, E.C., E.D.C. and A.R.F.; writing—review and editing, E.C., E.D.C. and C.D.N.; supervision, A.R.F. and G.M. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Informed Consent Statement

Not applicable.

Data Availability Statement

Data are available upon reasonable request.

Conflicts of Interest

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

References

  1. Sade, J. Treatment of cholesteatoma and retraction pockets. Eur. Arch. Otorhinol. 1993, 250, 193–199. [Google Scholar] [CrossRef] [PubMed]
  2. Skoloudik, L.; Simakova, E.; Kalfert, D.; Chrobok, V. Histological changes of the middle ear ossicles harvested during cholesteatoma surgery. Acta Med. 2015, 58, 119–122. [Google Scholar]
  3. Kuo, C.L.; Shiao, A.S.; Yung, M.; Sakagami, M.; Sudhoff, H.; Wang, C.H.; Hsu, C.H.; Lien, C.F. Updates and knowledge gaps in cholesteatoma research. BioMed Res. Int. 2015, 2015, 854024. [Google Scholar] [CrossRef] [PubMed]
  4. Kennedy, K.L.; Singh, A.K. Middle Ear Cholesteatoma; StatPearls Publishing: Tampa, FL, USA, 2022. [Google Scholar]
  5. Eggink, M.C.; de Wolf, M.J.F.; Ebbens, F.A.; Dikkers, F.G.; van Spronsen, E. Evaluation of Long-term Cholesteatoma Recidivism: Using the JOS, EAONO-JOS, and STAMCO Cholesteatoma Staging Systems. Otol. Neurotol. 2021, 42, 1031–1038. [Google Scholar] [CrossRef]
  6. Jennings, B.A.; Prinsley, P.; Philpott, C.; Willis, G.; Bhutta, M.F. The genetics of cholesteatoma. A systematic review using narrative synthesis. Clin. Otolaryngol. 2018, 43, 55–67. [Google Scholar] [CrossRef] [Green Version]
  7. Kaya, I.; Avcı, B.; Şahin, F.F.; Özateş, N.P.; Sezgin, B.; Kurt, C.; Bilgen, C.; Kirazlı, T. Evaluation of significant gene expression changes in congenital and acquired cholesteatoma. Mol. Biol. Rep. 2020, 47, 6127–6133. [Google Scholar] [CrossRef]
  8. Kan, T.; Ueda, H.; Takahara, H.; Tsuchiya, Y.; Kishimoto, M.; Uchida, Y.; Ogawa, T.; Ohashi, W.; Tsuzuki, T.; Fujimoto, Y. Association of Matrix Metalloproteinase-2 mRNA Expression with Subtypes of Pediatric Cholesteatoma. BioMed Res. Int. 2021, 2021, 6644897. [Google Scholar] [CrossRef]
  9. Maniu, A.; Harabagiu, O.; Perde Schrepler, M.; Cătană, A.; Fănuţă, B.; Mogoantă, C.A. Molecular biology of cholesteatoma. Rom. J. Morphol. Embryol. 2014, 55, 7–13. [Google Scholar]
  10. Aimi, K. Role of the tympanic ring in the pathogenesis of congenital cholesteatoma. Laryngoscope 1983, 93, 1140–1146. [Google Scholar] [CrossRef]
  11. Michaels, L. An epidermoid formation in the developing middle ear: Possible source of cholesteatoma. J. Otolaryngol. 1986, 15, 169–174. [Google Scholar]
  12. Michaels, L. Origin of congenital cholesteatoma from a normally occurring epidermoid rest in the developing middle ear. Int. J. Pediatr. Otorhinolaryngol. 1988, 15, 51–65. [Google Scholar] [CrossRef]
  13. Kodama, K.; Hara, M.; Hasegawa, M.; Matsuzawa, S.; Shinnabe, M.; Kanazawa, H.; Yoshida, N.; Iino, Y. Two cases of spontaneous regression of congenital cholesteatomas. Int. J. Pediatr. Otorhinolaryngol. 2012, 76, 142–144. [Google Scholar] [CrossRef] [PubMed]
  14. Yamamoto-Fukuda, T.; Aoki, D.; Hishikawa, Y.; Kobayashi, T.; Takahashi, H.; Koji, T. Possible involvement of keratinocyte growth factor and its receptor in enhanced epithelial-cell proliferation and acquired recurrence of middle-ear cholesteatoma. Lab. Investig. 2003, 83, 123–136. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  15. Araz Server, E.; Kalaycık Ertugay, Ç.; Baykal Koca, S.; Longur, E.S.; Yiğit, Ö.; Demirhan, H.; Çakır, Y. Predictive Role of Ki-67 and Proliferative-Cell Nuclear Antigen (PCNA) in Recurrent Cholesteatoma. J. Int. Adv. Otol. 2019, 15, 38–42. [Google Scholar] [CrossRef] [PubMed]
  16. Palkó, E.; Póliska, S.; Sziklai, I.; Penyige, A. Analysis of KRT1, KRT10, KRT19, TP53 and MMP9 expression in pediatric and adult cholesteatoma. PLoS ONE 2018, 13, e0200840. [Google Scholar] [CrossRef] [PubMed]
  17. Si, Y.; Chen, Y.B.; Chen, S.J.; Zheng, Y.Q.; Liu, X.; Liu, Y.; Jiang, H.L.; Xu, G.; Li, Z.H.; Huang, Q.H.; et al. TLR4 drives the pathogenesis of acquired cholesteatoma by promoting local inflammation and bone destruction. Sci. Rep. 2015, 5, 16683. [Google Scholar] [CrossRef] [Green Version]
  18. Imai, R.; Sato, T.; Iwamoto, Y.; Hanada, Y.; Terao, M.; Ohta, Y.; Osaki, Y.; Imai, T.; Morihana, T.; Okazaki, S.; et al. Osteoclasts Modulate Bone Erosion in Cholesteatoma via RANKL Signaling. J. Assoc. Res. Otolaryngol. 2019, 20, 449–459. [Google Scholar] [CrossRef]
  19. Wu, Y.; Tang, X.; Shao, W.; Lu, Y. Effect of CT manifestations of cholesteatoma on MMP-2, MMP-9 and IL-6 in the serum of patients. Exp. Ther. Med. 2019, 17, 4441–4446. [Google Scholar] [CrossRef] [Green Version]
  20. Haruyama, T.; Furukawa, M.; Kusunoki, T.; Onoda, J.; Ikeda, K. Expression of IL-17 and its role in bone destruction in human middle ear cholesteatoma. ORL J. Otorhinolaryngol. Relat. Spec. 2010, 72, 325–331. [Google Scholar] [CrossRef]
  21. Artono; Surarto, B.; Purnami, N.; Hutahaen, F.; Mahardhika, M.R. The Association of IL-1 Alpha Level and TNF Alpha Expressions on Bone Destruction in Chronic Suppurative Otitis Media and Cholesteatoma. Indian J. Otolaryngol. Head Neck Surg. 2020, 72, 1–7. [Google Scholar] [CrossRef]
  22. Choufani, G.; Ghanooni, R.; Decaestecker, C.; Delbrouck, K.; Simon, P.; Schüring, M.P.; Zick, Y.; Hassid, S.; Gabius, H.J.; Kiss, R. Detection of macrophage migration inhibitory factor (MIF) in human cholesteatomas and functional implications of correlations to recurrence status and to expression of matrix metalloproteinases-3/9, retinoic acid receptor-beta, and anti-apoptotic galectin-3. Laryngoscope 2001, 111, 1656–1662. [Google Scholar] [CrossRef] [PubMed]
  23. Moher, D.; Liberati, A.; Tetzlaff, J.; Altman, D.G.; PRISMA Group. Preferred reporting items for systematic reviews and meta-analyses: The PRISMA statement. BMJ 2009, 339, b2535. [Google Scholar] [CrossRef] [Green Version]
  24. Perlikos, F.; Hillas, G.; Loukides, S. Phenotyping and Endotyping Asthma Based on Biomarkers. Curr. Top. Med. Chem. 2016, 16, 1582–1586. [Google Scholar] [CrossRef] [PubMed]
  25. Teitelbaum, S.L.; Ross, F.P. Genetic regulation of osteoclast development and function. Nat. Rev. Genet. 2003, 4, 638–649. [Google Scholar] [CrossRef] [PubMed]
  26. Kim, K.W.; Cho, M.L.; Lee, S.H.; Oh, H.J.; Kang, C.M.; Ju, J.H.; Min, S.Y.; Cho, Y.G.; Park, S.H.; Kim, H.Y. Human rheumatoid synovial fibroblasts promote osteoclastogenic activity by activating RANKL via TLR-2 and TLR-4 activation. Immunol. Lett. 2007, 110, 54–64. [Google Scholar] [CrossRef]
  27. Lundy, S.K.; Sarkar, S.; Tesmer, L.A.; Fox, D.A. Cells of the synovium in rheumatoid arthritis. T lymphocytes. Arthritis Res. Ther. 2007, 9, 202. [Google Scholar] [CrossRef] [Green Version]
  28. Fokkens, W.J.; Lund, V.J.; Hopkins, C.; Hellings, P.W.; Kern, R.; Reitsma, S.; Toppila-Salmi, S.; Bernal-Sprekelsen, M.; Mullol, J. Executive summary of EPOS 2020 including integrated care pathways. Rhinology 2020, 58, 82–111. [Google Scholar] [CrossRef]
  29. Suchozebrska-Jesionek, D.; Szymański, M.; Kurzepa, J.; Gołabek, W.; Stryjecka-Zimmer, M. Gelatinolytic activity of matrix metalloproteinases 2 and 9 in middle ear cholesteatoma. J. Otolaryngol. Head Neck Surg. 2008, 37, 628–632. [Google Scholar]
  30. Wang, L.F.; Chien, C.Y.; Tai, C.F.; Kuo, W.R.; His, E.; Juo, S.H. Matrix metalloproteinase-9 gene polymorphisms in nasal polyposis. BMC Med. Genet. 2010, 11, 85. [Google Scholar] [CrossRef] [Green Version]
  31. Rysz, J.; Banach, M.; Stolarek, R.A.; Pasnik, J.; Cialkowska-Rysz, A.; Koktysz, R.; Piechota, M.; Baj, Z. Serum matrix metalloproteinases MMP-2 and MMP-9 and metalloproteinase tissue inhibitors TIMP-1 and TIMP-2 in diabetic nephropathy. J. Nephrol. 2007, 20, 444–452. [Google Scholar]
  32. Yamamoto-Fukuda, T.; Akiyama, N.; Tatsumi, N.; Okabe, M.; Kojima, H. Menin-MLL inhibitor blocks progression of middle ear cholesteatoma in vivo. Int. J. Pediatr. Otorhinolaryngol. 2021, 140, 110545. [Google Scholar] [CrossRef]
  33. Agache, I.; Akdis, C.A. Endotypes of allergic diseases and asthma: An important step in building blocks for the future of precision medicine. Allergol. Int. 2016, 65, 243–252. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  34. De Corso, E.; Montuori, C.; Settimi, S.; Mele, D.A.; Cantiani, A.; Corbò, M.; Cantone, E.; Paludetti, G.; Galli, J. Efficacy of Biologics on Refractory Eosinophilic Otitis Media Associated with Bronchial Asthma or Severe Uncontrolled CRSwNP. J. Clin. Med. 2022, 11, 926. [Google Scholar] [CrossRef] [PubMed]
  35. Lou, H.; Wang, C.; Zhang, L. Endotype-driven precision medicine in chronic rhinosinusitis. Expert Rev. Clin. Immunol. 2019, 15, 1171–1183. [Google Scholar] [CrossRef] [PubMed]
  36. Passalacqua, G.; Ciprandi, G.; Canonica, G.W. The nose-lung interaction in allergic rhinitis and asthma: United airways disease. Curr. Opin. Allergy Clin. Immunol. 2001, 1, 7–13. [Google Scholar] [CrossRef]
  37. Kuo, C.L.; Yen, Y.C.; Chang, W.P.; Shiao, A.S. Association Between Middle Ear Cholesteatoma and Chronic Rhinosinusitis. JAMA Otolaryngol. Head Neck Surg. 2017, 143, 757–763. [Google Scholar] [CrossRef]
  38. Nguyen, L.H.; Manoukian, J.J.; Sobol, S.E.; Tewfik, T.L.; Mazer, B.D.; Schloss, M.D.; Taha, R.; Hamid, Q.A. Similar allergic inflammation in the middle ear and the upper airway: Evidence linking otitis media with effusion to the united airways concept. J. Allergy Clin. Immunol. 2004, 114, 1110–1115. [Google Scholar] [CrossRef]
Jpm 12 01347 g001 550
Figure 1. Prisma flow diagram of the systematic search.
Figure 1. Prisma flow diagram of the systematic search.
Jpm 12 01347 g001
Table
Table 1. Studies on molecular biomarkers of Cholesteatoma.
Table 1. Studies on molecular biomarkers of Cholesteatoma.
Author Year Ref.N. of Cases (Sex; Mean Age)MethodsSample TypeAnalytical Technique Relevant ResultsRecurrenceBone Erosion
Yamamoto-Fukuda et al. 2003 [14]A total of 56 cases (32 M, 24 F; mean age:35)Expression of KGF, KGFR, Ki-67Soft tissueImmunohistochemical analysis and electroporatically transfection to the mice ears KGF were detected in 80% of C, KGFR in 72%. Higher Ki-67 labeling index (66%) in KGF+/KGFR+ cases than other cases. Correlation between KGF+/KGFR+ expression and recurrence.Y
Araz Server et al. 2019 [15]A total of 43 cases (29 M, 14 F; mean age: 33)Two groups: recurrent (21 patients) and non-recurrent within 2 years (22 patients). Ki-67 and the percentages of stained cells were calculated.Bone tissueImmunohistochemical analysisA significant relationship between Ki-67 staining and malleus involvement Y
Palkó et al. 2018 [16]A total of 26, 15 children and 11 adult (16 M, 11 F; mean age 23,4)The expression of three cytokeratin genes (KRT1, KRT10 and KRT19), the matrix metalloproteinase 9 gene (MMP9) and the tumor suppressor TP53 gene was measured by qRT-PCR in surgical samples of cholesteatoma (C) cases and their expression level was compared to that of normal skin samplesSoft tissuemRNA expressionResults showed identical expression pattern for KRT1 and KRT10, their expression was higher in pediatric cases than in adults, especially in pediatric recurrent samples. The expression level of KRT19 was lower in the more invasive recurrent cases both in our pediatric and adult groups. expression of MMP9 was highest in adult recurrent casesY
Si et al. 2015 [17]A total of 187 specimens of acquired cholesteatoma (137 F and 51 M, mean age: 45)Immunohistochemical analysis of TLR4 expression was performed in cholesteatoma specimen.Congenital cholesteatomas and acquired cholesteatomas samplesReal-timePCR, Western blotting, and immunohistochemistry.
Animal models		The number of TLR4-positive cells increased with an increased degree of cholesteatoma invasion, bone destruction, and hearing loss Y
Imai et al. 2019 [18]A total of 24 cholesteatoma specimens from patients who underwent tympanomastoidectomy.Osteoclasts were stained using tartrate-resistant acid phosphatase (TRAP)-hematoxylin counterstaining. The expression of RANKL mRNA was performed with the droplet digital polymerase chain reaction (ddPCR) system.Bone-soft tissuesImmunohistochemistry, RNA Sequencing and ELISAThe number of osteoclasts on the bone surface adjacent to C was significantly larger than on the surface of control bone. The expression of RANKL mRNA was significantly higher in cholesteatomas than in control skin. RANKL was expressed in fibroblasts in the cholesteatoma perimatrix. Y
Wu et al. 2019 [19]A total of 176 patients (91 M and 85 F, mean age 49, 65 ± 5, 27 with cholesteatomatous otitis mediaThe expression levels of MMP-2, MMP-9 and IL-6 in the serum of the selected patients were detected by ELISASerumELISAMMP-2, MMP-9 and IL-6 were higly expressed in the serum of patients with C compared to the control group and were positively correlated with CT manifestations of the patients and the injury degree of ossicle, which may be a sign of poor prognosis of cholesteatomatous otitis media (COM). Y
Haruyama et al. 2010 [20]Tissue specimens collected from 24 patients with cholesteatomas (16 M and 8 F, mean age: 44); congenital in 8 patients and acquired in 16 patients.The expression and localization of IL17 and RANKL were examined by immunohistochemistry in tissue specimens. The cellular sources of IL-17 were assessed by double immunofluorescent staining with CD4. The level of IL-17 protein was determined using an enzyme-linked immunosorbent assay. The degree of bone destruction was compared with the IL-17 immunoreactivityCholesteatoma tissueImmunohistochemistryIL-17-positive inflammatory cells were seen in the subepithelial granulation tissue but not in the epithelium of the C. The localization of IL-17 expression coincided with CD4-positive lymphocytes. The subepithelial granulation had RANKL positive infiltrating cells and a significant correlation between IL-17- and RANKL-positive cells in the same specimens was recognized. The degree of bone destruction was dependent on the number of IL-17-positive cells that infiltrated the cholesteatoma. Y
Artono et al. 2019 [21]A total of 46 patients (26 M and 20 F, age range of 21–30 years) with chronic suppurative otitis media (CSOM) with cholesteatomaPathological tissue in the form of cholesteatoma tissue and external acustic meatus skin during surgery was assessed. IL-1a expression was assessed by using ELISA. TNF-a expression was determined by immunohistochemical staining.Cholesteatoma tissueELISAThere is a significant association between expression of TNF-a and IL-1a level on the severity of bone destruction in CSOM and C patients Y
Choufani et al. 2001 [22]A total of 56 C specimens (33 M and 23 F), and 5 congenital and 51 acquired cases.The immunohistochemical levels of
expression of macrophage migration inhibitory factor (MIF) was statistically correlated to parameters of the
other markers (galectin 1, -3, and -8, retinoid acid
receptors (RAR) binding sites for sarcolectin,
and invasion markers (cathepsins -B and -D,
and matrix metalloproteinases [MMP]-2, -3, and -9)		Cholesteatoma tissueImmunohistochemistryMIF expression is higher in recurrent than in nonrecurrent cholesteatomas. MIF expression in infected ones it is correlated to MMP-3 and galectin-3 expression.Y
Table
Table 2. Molecular biomarkers of recidivism.
Table 2. Molecular biomarkers of recidivism.
Molecular Biomarkers
KGF-KGFRYamamoto et al. 2003 [14]
MMP-9Palkó et al. 2018 [16]
KRT-1Palkó et al. 2018 [16]
KRT-10Palkó et al. 2018 [16]
MIFChoufani et al. 2001 [22]
Table
Table 3. Molecular biomarkers of bone erosion.
Table 3. Molecular biomarkers of bone erosion.
Molecular Biomarkers
Ki-67Araz-Server et al. 2019 [15]
TLR-4Si et al. 2015 [17]
RANKLImai et al. 2019 [18]
IL-17Haruyama et al. 2010 [20]
MMP-2 (serum)Wu et al. 2019 [19]
MMP-9 (serum)Wu et al. 2019 [19]
IL-6 (serum)Wu et al. 2019 [19]
TNF-αArtono et al. 2019 [21]
IL-1αArtono et al. 2019 [21]
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Cantone, E.; Di Nola, C.; De Corso, E.; Cavaliere, M.; Grimaldi, G.; Fetoni, A.R.; Motta, G. Endotyping of Cholesteatoma: Which Molecular Biomarkers? A Systematic Review. J. Pers. Med. 2022, 12, 1347. https://doi.org/10.3390/jpm12081347

AMA Style

Cantone E, Di Nola C, De Corso E, Cavaliere M, Grimaldi G, Fetoni AR, Motta G. Endotyping of Cholesteatoma: Which Molecular Biomarkers? A Systematic Review. Journal of Personalized Medicine. 2022; 12(8):1347. https://doi.org/10.3390/jpm12081347

Chicago/Turabian Style

Cantone, Elena, Claudio Di Nola, Eugenio De Corso, Michele Cavaliere, Giusi Grimaldi, Anna Rita Fetoni, and Gaetano Motta. 2022. "Endotyping of Cholesteatoma: Which Molecular Biomarkers? A Systematic Review" Journal of Personalized Medicine 12, no. 8: 1347. https://doi.org/10.3390/jpm12081347

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop