Association of LINC00673 Genetic Variants with Progression of Oral Cancer
Abstract
:1. Introduction
2. Materials and Methods
2.1. Subjects
2.2. Selection and Genotyping of LINC00673 SNPs
2.3. Bioinformatics Analysis
2.4. Statistical Analysis
3. Results
3.1. Cohort Characteristics
3.2. Association of LINC00673 Gene Polymorphism with the Progression of OSCC
3.3. Clinical and Functional Insights of LINC00673 into OSCC
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Variable | Controls (N = 1194) | Patients (N = 1231) | p-Value |
---|---|---|---|
Age (yrs) | 53.90 ± 10.03 | 55.53 ± 10.85 | |
<55 | 565 (47.3%) | 574 (46.6%) | p = 0.733 |
≥55 | 629 (52.7%) | 657 (53.4%) | |
Betel quid chewing | |||
No | 995 (83.3%) | 303 (24.6%) | |
Yes | 199 (16.7%) | 928 (75.4%) | p < 0.001 * |
Cigarette smoking | |||
No | 558 (46.7%) | 193 (15.7%) | |
Yes | 636 (53.3%) | 1038 (84.3%) | p < 0.001 * |
Alcohol drinking | |||
No | 957 (80.2%) | 648 (52.6%) | |
Yes | 237 (19.8%) | 583 (47.4%) | p < 0.001 * |
Stage | |||
I + II | 575 (46.7%) | ||
III + IV | 656 (53.3%) | ||
Tumor T status | |||
T1 + T2 | 605 (49.1%) | ||
T3 + T4 | 626 (50.9%) | ||
Lymph node status | |||
N0 | 804 (65.3%) | ||
N1 + N2 + N3 | 427 (34.7%) | ||
Metastasis | |||
M0 | 1220 (99.1%) | ||
M1 | 11 (0.9%) | ||
Cell differentiation | |||
Well differentiated | 174 (14.1%) | ||
Moderately or poorly differentiated | 1057 (85.9%) |
Variable | Controls (N = 1194) n (%) | Patients (N = 1231) n (%) | OR (95% CI) | AOR (95% CI) |
---|---|---|---|---|
rs11655237 | ||||
CC | 772 (64.7%) | 784 (63.7%) | 1.000 | 1.000 |
CT | 371 (31.1%) | 398 (32.3%) | 1.056 (0.889–1.256) | 1.034 (0.834–1.282) |
TT | 51 (4.2%) | 49 (4.0%) | 0.946 (0.631–1.418) | 0.675 (0.405–1.125) |
CT + TT | 422 (35.3%) | 447 (36.3%) | 1.043 (0.883–1.231) | 0.986 (0.802–1.213) |
rs9914618 | ||||
GG | 770 (64.5%) | 799 (64.9%) | 1.000 (reference) | 1.000 (reference) |
GA | 370 (31.0%) | 380 (30.9%) | 0.990 (0.832–1.178) | 0.954 (0.768–1.184) |
AA | 54 (4.5%) | 52 (4.2%) | 0.928 (0.626–1.375) | 0.652 (0.398–1.068) |
GA + AA | 424 (35.5%) | 432 (35.1%) | 0.982 (0.831–1.160) | 0.911 (0.740–1.121) |
rs6501551 | ||||
AA | 890 (74.5%) | 919 (74.7%) | 1.000 (reference) | 1.000 (reference) |
AG | 279 (23.4%) | 294 (23.9%) | 1.021 (0.846–1.232) | 1.039 (0.822–1.312) |
GG | 25 (2.1%) | 18 (1.4%) | 0.697 (0.378–1.287) | 0.689 (0.323–1.471) |
AG + GG | 304 (25.5%) | 312 (25.3%) | 0.994 (0.828–1.193) | 0.911 (0.740–1.121) |
Variable | LINC00673 (rs9914618) | |||
---|---|---|---|---|
GG (%) (n = 799) | GA + AA (%) (n = 432) | OR (95% CI) | p-Value | |
Clinical Stage | ||||
Stage I/II | 373 (46.7%) | 202 (46.8%) | 1.00 | p = 0.980 |
Stage III/IV | 426 (53.3%) | 230 (53.2%) | 0.997 (0.788–1.261) | |
Tumor size | ||||
T1 + T2 | 393 (49.2%) | 212 (49.1%) | 1.00 | p = 0.970 |
T3 + T4 | 406 (50.8%) | 220 (50.9%) | 1.005 (0.795–1.270) | |
Lymph node metastasis | ||||
No | 538 (67.3%) | 266 (61.6%) | 1.00 | p = 0.043 |
Yes | 261 (32.7%) | 166 (38.4%) | 1.286 (1.008–1.642) | |
Distant metastasis | ||||
No | 794 (99.4%) | 426 (98.6%) | 1.00 | p = 0.175 |
Yes | 5 (0.6%) | 6 (1.4%) | 2.237 (0.679–7.371) | |
Cell differentiation | ||||
well | 111 (13.9%) | 63 (14.6%) | 1.00 | p = 0.740 |
Moderate/poor | 688 (86.1%) | 369 (85.4%) | 0.945 (0.677–1.320) |
Variable | LINC00673 (rs9914618) | |||||
---|---|---|---|---|---|---|
Betel Quid Chewing (n = 928) | Smoking (n = 1038) | |||||
GG (%) (n = 590) | GA + AA (%) (n = 338) | p-Value | GG (%) (n = 672) | GA + AA (%) (n = 366) | p-Value | |
Clinical Stage | ||||||
Stage I/II | 288 (48.8%) | 148 (43.8%) | p = 0.140 | 318 (47.3%) | 165 (45.1%) | p = 0.489 |
Stage III/IV | 302 (51.2%) | 190 (56.2%) | 354 (52.7%) | 201 (54.9%) | ||
Tumor size | ||||||
≤T2 | 300 (50.8%) | 163 (48.2%) | p = 0.442 | 345 (51.3%) | 180 (49.2%) | p = 0.506 |
>T2 | 290 (49.2%) | 175 (51.8%) | 327 (48.7%) | 186 (50.8%) | ||
Lymph node metastasis | ||||||
No | 410 (69.5%) | 202 (59.8%) | p = 0.003 *,a | 454 (67.6%) | 224 (61.2%) | p = 0.040 *,b |
Yes | 180 (30.5%) | 136 (40.2%) | 218 (32.4%) | 142 (38.8%) | ||
Distant metastasis | ||||||
No | 586 (99.3%) | 334 (98.8%) | p = 0.423 | 668 (99.4%) | 360 (98.4%) | p = 0.100 |
Yes | 4 (0.7%) | 4 (1.2%) | 4 (0.6%) | 6 (1.6%) | ||
Cell differentiation | ||||||
well | 94 (15.9%) | 47 (13.9%) | p = 0.408 | 97 (14.4%) | 59 (16.1%) | p = 0.468 |
Moderate/poor | 496 (84.1%) | 291 (86.1%) | 575 (85.6%) | 307 (83.9%) |
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Su, S.-C.; Lin, C.-W.; Ju, P.-C.; Chang, L.-C.; Chuang, C.-Y.; Liu, Y.-F.; Hsieh, M.-J.; Yang, S.-F. Association of LINC00673 Genetic Variants with Progression of Oral Cancer. J. Pers. Med. 2021, 11, 468. https://doi.org/10.3390/jpm11060468
Su S-C, Lin C-W, Ju P-C, Chang L-C, Chuang C-Y, Liu Y-F, Hsieh M-J, Yang S-F. Association of LINC00673 Genetic Variants with Progression of Oral Cancer. Journal of Personalized Medicine. 2021; 11(6):468. https://doi.org/10.3390/jpm11060468
Chicago/Turabian StyleSu, Shih-Chi, Chiao-Wen Lin, Po-Chung Ju, Lun-Ching Chang, Chun-Yi Chuang, Yu-Fan Liu, Ming-Ju Hsieh, and Shun-Fa Yang. 2021. "Association of LINC00673 Genetic Variants with Progression of Oral Cancer" Journal of Personalized Medicine 11, no. 6: 468. https://doi.org/10.3390/jpm11060468