Evaluation of the Accuracy of Estimated Endpoint Titer of NOVA View in Indirect Immunofluorescent Antinuclear Antibody Testing
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Design
2.2. Detection of ANA and Determination of Endpoint Titer by Serial Dilution and Estimation Function
2.3. Statistical Analysis
3. Results
3.1. Patient Characteristics
3.2. Relationship between Light Intensity Units and Endpoint Titers
3.3. Concordance of dEPT and eEPT
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Total n = 1518 (%) | Autoimmune Disease n = 697 (%) | Non-Autoimmune Disease n = 821 (%) | p Value | |
---|---|---|---|---|
Age, year [1st–3rd interquartile range] | 57.0 [41.0–67.0] | 53.0 [39.0–64.0] | 59.0 [43.0–70.0] | <0.001 |
Female | 1161 (76.5) | 589 (84.5) | 572 (69.7) | <0.001 |
Underlying disease | ||||
Hypertension | 92 (6.1) | 43 (6.2) | 59 (7.2) | 0.493 |
Diabetes mellitus | 64 (4.2) | 28 (4.0) | 36 (4.4) | 0.820 |
Chronic kidney disease | 81 (5.3) | 25 (3.6) | 56 (6.8) | 0.007 |
Malignancy | 53 (3.5) | 8 (1.1) | 45 (5.5) | <0.001 |
Autoantibody Pattern | 0.019 | |||
Homogeneous | 632 (41.6) | 265 (38.0) | 367 (44.7) | |
Speckled | 669 (44.1) | 319 (45.8) | 350 (42.6) | |
Nucleolar | 68 (4.5) | 29 (4.2) | 39 (4.8) | |
Centromere | 129 (8.5) | 73 (10.5) | 56 (6.8) | |
Nuclear dots | 20 (1.3) | 11 (1.6) | 9 (1.1) | |
Endpoint titer of autoantibody by dilution | <0.001 | |||
1:80 | 786 (51.8) | 248 (35.6) | 538 (65.5) | |
1:160 | 308 (20.3) | 145 (20.8) | 163 (19.9) | |
1:320 | 151 (9.9) | 87 (12.5) | 64 (7.8) | |
≥1:640 | 273 (18.0) | 217 (31.1) | 56 (6.8) |
Total (n = 1518) | Speckled Pattern (n = 669) | Homogeneous Pattern (n = 632) | Centromere Pattern (n = 129) | Nucleolar Pattern (n = 68) | Nuclear Dot Pattern (n = 20) | |
---|---|---|---|---|---|---|
Before adjustment | ||||||
Exact match, n (%) | 336 (22.1) | 213 (31.8) | 58 (9.2) | 79 (61.2) | 10 (14.7) | 10 (50.0) |
±One-titer match, n (%) | 699 (46.0) | 402 (60.1) | 200 (31.6) | 81 (62.8) | 38 (55.9) | 12 (60.0) |
Categorical match, n (%) | 780 (51.4) | 345 (51.6) | 259 (41.0) | 119 (92.3) | 49 (61.2) | 16 (80.0) |
After downgraded adjustment of eEPT * | ||||||
Exact match, n (%) | 774 (51.0) | 290 (43.4) | 353 (66.4) | 87 (67.4) | 29 (42.7) | 9 (45.0) |
±One-titer match, n (%) | 1412 (93.0) | 576 (86.1) | 500 (94.0) | 124 (96.1) | 61 (89.7) | 16 (80.0) |
Categorical match, n (%) | 1267 (83.5) | 579 (86.6) | 397 (74.6) | 121 (93.8) | 49 (72.1) | 18 (90.0) |
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Cho, H.W.; Jeong, S.-H.; Hong, J.S.; Kim, D.; Park, Y.; Jeong, S.H. Evaluation of the Accuracy of Estimated Endpoint Titer of NOVA View in Indirect Immunofluorescent Antinuclear Antibody Testing. Diagnostics 2024, 14, 1580. https://doi.org/10.3390/diagnostics14151580
Cho HW, Jeong S-H, Hong JS, Kim D, Park Y, Jeong SH. Evaluation of the Accuracy of Estimated Endpoint Titer of NOVA View in Indirect Immunofluorescent Antinuclear Antibody Testing. Diagnostics. 2024; 14(15):1580. https://doi.org/10.3390/diagnostics14151580
Chicago/Turabian StyleCho, Hae Weon, Soon-Ho Jeong, Jun Sung Hong, Dokyun Kim, Yongjung Park, and Seok Hoon Jeong. 2024. "Evaluation of the Accuracy of Estimated Endpoint Titer of NOVA View in Indirect Immunofluorescent Antinuclear Antibody Testing" Diagnostics 14, no. 15: 1580. https://doi.org/10.3390/diagnostics14151580
APA StyleCho, H. W., Jeong, S.-H., Hong, J. S., Kim, D., Park, Y., & Jeong, S. H. (2024). Evaluation of the Accuracy of Estimated Endpoint Titer of NOVA View in Indirect Immunofluorescent Antinuclear Antibody Testing. Diagnostics, 14(15), 1580. https://doi.org/10.3390/diagnostics14151580