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Negative mpMRI Rules Out Extra-Prostatic Extension in Prostate Cancer before Robot-Assisted Radical Prostatectomy
 
 
Article

Ex Vivo Fluorescence Confocal Microscopy (FCM) of Prostate Biopsies Rethought: Opportunities of Intraoperative Examinations of MRI-Guided Targeted Biopsies in Routine Diagnostics

1
Department of Urology, University Hospital OWL of the University of Bielefeld, Campus Lippe, 32756 Detmold, Germany
2
Institute of Pathology, University Hospital OWL of the University of Bielefeld, Campus Lippe, 32756 Detmold, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Shonit Punwani
Diagnostics 2022, 12(5), 1146; https://doi.org/10.3390/diagnostics12051146
Received: 3 April 2022 / Revised: 2 May 2022 / Accepted: 3 May 2022 / Published: 5 May 2022
(This article belongs to the Special Issue MRI in Prostate Cancer: New Evidences and Future Perspectives)
Background: The diagnosis of prostate carcinoma (PCa) requires time- and material-consuming histopathological examinations. Ex vivo fluorescence confocal microscopy (FCM) can detect carcinoma foci in diagnostic biopsies intraoperatively. Methods: MRI-guided and systematic biopsies were identified in a dataset of our previously published study cohort. Detection rates of clinically relevant tumors were determined in both groups. A retrospective blinded trial was performed to determine how many tumors requiring intervention were detectable via FCM analysis of MRI-guided targeted biopsies alone. Results: MRI-guided targeted biopsies revealed tumors more frequently than systematic biopsies. Carcinomas in need of intervention were reliably represented in the MRI-guided biopsies and were identified in intraoperative FCM microscopy. Combined with serum PSA levels and clinical presentation, 91% of the carcinomas in need of intervention were identified. Conclusions: Intraoperative FCM analysis of MRI-guided biopsies is a promising approach for the efficient diagnosis of PCa. The method allows a timely assessment of whether a tumor disease requiring intervention is present and can reduce the psychological stress of the patient in the waiting period of the histological finding. Furthermore, this technique can lead to reduction of the total number of biopsies needed for the diagnosis of PCa. View Full-Text
Keywords: confocal microscopy; prostate cancer; targeted biopsies; digital pathology confocal microscopy; prostate cancer; targeted biopsies; digital pathology
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MDPI and ACS Style

Sievert, K.-D.; Hansen, T.; Titze, B.; Schulz, B.; Omran, A.; Brockkötter, L.; Gunnemann, A.; Titze, U. Ex Vivo Fluorescence Confocal Microscopy (FCM) of Prostate Biopsies Rethought: Opportunities of Intraoperative Examinations of MRI-Guided Targeted Biopsies in Routine Diagnostics. Diagnostics 2022, 12, 1146. https://doi.org/10.3390/diagnostics12051146

AMA Style

Sievert K-D, Hansen T, Titze B, Schulz B, Omran A, Brockkötter L, Gunnemann A, Titze U. Ex Vivo Fluorescence Confocal Microscopy (FCM) of Prostate Biopsies Rethought: Opportunities of Intraoperative Examinations of MRI-Guided Targeted Biopsies in Routine Diagnostics. Diagnostics. 2022; 12(5):1146. https://doi.org/10.3390/diagnostics12051146

Chicago/Turabian Style

Sievert, Karl-Dietrich, Torsten Hansen, Barbara Titze, Birte Schulz, Ahmad Omran, Lukas Brockkötter, Alfons Gunnemann, and Ulf Titze. 2022. "Ex Vivo Fluorescence Confocal Microscopy (FCM) of Prostate Biopsies Rethought: Opportunities of Intraoperative Examinations of MRI-Guided Targeted Biopsies in Routine Diagnostics" Diagnostics 12, no. 5: 1146. https://doi.org/10.3390/diagnostics12051146

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