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Diagnostics
Volume 12
Issue 1
10.3390/diagnostics12010032
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Article
Peer-Review Record

Advanced Multiplex Loop Mediated Isothermal Amplification (mLAMP) Combined with Lateral Flow Detection (LFD) for Rapid Detection of Two Prevalent Malaria Species in India and Melting Curve Analysis

Diagnostics 2022, 12(1), 32; https://doi.org/10.3390/diagnostics12010032
by Supriya Sharma, Sandeep Kumar, Md Zohaib Ahmed, Nitin Bhardwaj, Jaskirat Singh, Sarita Kumari, Deepali Savargaonkar, Anupkumar R. Anvikar * and Jyoti Das *
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Diagnostics 2022, 12(1), 32; https://doi.org/10.3390/diagnostics12010032
Submission received: 10 September 2021 / Revised: 22 October 2021 / Accepted: 25 October 2021 / Published: 24 December 2021
(This article belongs to the Special Issue Recent Advances in Malaria Diagnosis)

Round 1

Reviewer 1 Report

Please see the attached file.

Comments for author File: Comments.pdf

Author Response

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Author Response File: Author Response.docx

Reviewer 2 Report

In this study, authors standardized isothermal method (LAMP) as a malaria diagnostic kit. They combined isothermal method-LAMP with lateral flow device. Authors designed multiplex primers using the 18S SSU rRNA gene target region of Plasmodium, followed by visualization using Biotin-FITC and Biotin-Digoxigenin complex on the lateral chromatographic strip. Using the mLAMP-LFD method, authors studied 39 clinical samples and results were compared with other existing methods. This study is advance from the rest of the previous studies by mainly two ways- sensitivity and reduced primer number. Some of the minor comments are-

  1. Please make sure spacing between words are correct. One word spacing is missing in page 4 and line 146, e.g. wascarried.
  2. What is againg in page 10 and line 311. Is it against??
  3. Specify the DNA concentration used in material and methods 2.6. 

Author Response

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Author Response File: Author Response.docx

Round 2

Reviewer 1 Report

Please see the attached file.

Comments for author File: Comments.pdf

Author Response

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Author Response File: Author Response.docx

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