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Article

Five Commercial Immunoassays for SARS-CoV-2 Antibody Determination and Their Comparison and Correlation with the Virus Neutralization Test

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Department of Immunochemistry Diagnostics, University Hospital in Pilsen, 309 55 Pilsen, Czech Republic
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Laboratory of Immunology, Gennet, 110 00 Prague, Czech Republic
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Faculty of Science, Palacky University in Olomouc, 779 00 Olomouc, Czech Republic
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Department of Microbiology, University Hospital in Pilsen, 309 55 Pilsen, Czech Republic
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Department of Infectious Diseases and Travel Medicine, University Hospital in Pilsen, 309 55 Pilsen, Czech Republic
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Institute of Epidemiology, Faculty of Medicine in Pilsen, Charles University, 305 99 Pilsen, Czech Republic
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Department of Virology, Public Health Institute, 702 00 Ostrava, Czech Republic
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Department of Biomedical Sciences, Faculty of Medicine, University of Ostrava, 703 00 Ostrava, Czech Republic
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Department of Otorhinolaryngology, University Hospital in Pilsen, Faculty of Medicine in Pilsen, Charles University, 305 99 Pilsen, Czech Republic
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Institute of Pharmacology and Toxicology, Faculty of Medicine in Pilsen, Charles University, 323 00 Pilsen, Czech Republic
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Author to whom correspondence should be addressed.
Academic Editor: Sandeep K. Vashist
Diagnostics 2021, 11(4), 593; https://doi.org/10.3390/diagnostics11040593
Received: 4 March 2021 / Revised: 19 March 2021 / Accepted: 21 March 2021 / Published: 25 March 2021
(This article belongs to the Section Diagnostic Microbiology and Infectious Disease)
There is an ongoing debate as to whether SARS-CoV-2 antibodies can be found in patients who have recovered from COVID-19 disease. Currently, there is no consensus on whether the antibodies, if present, are protective. Our regular measurements of SARS-CoV-2 antibodies, starting in July 2020, have provided us with the opportunity of becoming acquainted with the five different immunoassays. A total of 149 patients were enrolled in our study. We measured the samples using each immunoassay, then performing a virus neutralization test and comparing the results of SARS-CoV-2 antibodies with this test. We observed that the production of neutralizing antibodies is age-dependent. Elderly patients have a higher proportion of high neutralizing titers than young patients. Based on our results, and in combination with the literature findings, we can conclude that the serological SARS-CoV-2 antibody measurement is a helpful tool in the fight against COVID-19. The assays can provide information about the patient’s previous contact with the virus. Anti-spike protein assays correlate well with the virus neutralization test and can be used in the screening of potential convalescent plasma donors. View Full-Text
Keywords: antibody; SARS-CoV-2; spike protein; nucleocapsid protein; serological diagnostics; immunoassay; virus neutralization test antibody; SARS-CoV-2; spike protein; nucleocapsid protein; serological diagnostics; immunoassay; virus neutralization test
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MDPI and ACS Style

Šimánek, V.; Pecen, L.; Krátká, Z.; Fürst, T.; Řezáčková, H.; Topolčan, O.; Fajfrlík, K.; Sedláček, D.; Šín, R.; Pazdiora, P.; Zelená, H.; Slouka, D.; Kučera, R. Five Commercial Immunoassays for SARS-CoV-2 Antibody Determination and Their Comparison and Correlation with the Virus Neutralization Test. Diagnostics 2021, 11, 593. https://doi.org/10.3390/diagnostics11040593

AMA Style

Šimánek V, Pecen L, Krátká Z, Fürst T, Řezáčková H, Topolčan O, Fajfrlík K, Sedláček D, Šín R, Pazdiora P, Zelená H, Slouka D, Kučera R. Five Commercial Immunoassays for SARS-CoV-2 Antibody Determination and Their Comparison and Correlation with the Virus Neutralization Test. Diagnostics. 2021; 11(4):593. https://doi.org/10.3390/diagnostics11040593

Chicago/Turabian Style

Šimánek, Václav, Ladislav Pecen, Zuzana Krátká, Tomáš Fürst, Hana Řezáčková, Ondřej Topolčan, Karel Fajfrlík, Dalibor Sedláček, Robin Šín, Petr Pazdiora, Hana Zelená, David Slouka, and Radek Kučera. 2021. "Five Commercial Immunoassays for SARS-CoV-2 Antibody Determination and Their Comparison and Correlation with the Virus Neutralization Test" Diagnostics 11, no. 4: 593. https://doi.org/10.3390/diagnostics11040593

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