Next Article in Journal
The Distinctive Regulation of Cyanobacterial Glutamine Synthetase
Next Article in Special Issue
Noise–Seeded Developmental Pattern Formation in Filamentous Cyanobacteria
Previous Article in Journal
Ultraviolet Irradiation on a Pyrite Surface Improves Triglycine Adsorption
Previous Article in Special Issue
The Ser/Thr Kinase PknH Is Essential for Maintaining Heterocyst Pattern in the Cyanobacterium Anabaena sp. Strain PCC 7120
Article Menu

Export Article

Open AccessCommunication

Biosensors-Based In Vivo Quantification of 2-Oxoglutarate in Cyanobacteria and Proteobacteria

Aix Marseille Univ, CNRS, LCB, IMM, 13009 Marseille, France
Author to whom correspondence should be addressed.
Received: 29 September 2018 / Revised: 24 October 2018 / Accepted: 25 October 2018 / Published: 27 October 2018
(This article belongs to the Special Issue Developmental Biology in Cyanobacteria)
PDF [2860 KB, uploaded 27 October 2018]
  |     |  


2-oxoglutarate (α-ketoglutarate; 2-OG) is an intermediate of the Krebs cycle, and constitutes the carbon skeleton for nitrogen assimilation and the synthesis of a variety of compounds. In addition to being an important metabolite, 2-OG is a signaling molecule with a broad regulatory repertoire in a variety of organisms, including plants, animals, and bacteria. Although challenging, measuring the levels and variations of metabolic signals in vivo is critical to better understand how cells control specific processes. To measure cellular 2-OG concentrations and dynamics, we designed a set of biosensors based on the fluorescence resonance energy transfer (FRET) technology that can be used in vivo in different organisms. For this purpose, we took advantage of the conformational changes of two cyanobacterial proteins induced by 2-OG binding. We show that these biosensors responded immediately and specifically to different 2-OG levels, and hence allowed to measure 2-OG variations in function of environmental modifications in the proteobacterium Escherichia coli and in the cyanobacterium Anabaena sp. PCC 7120. Our results pave the way to study 2-OG dynamics at the cellular level in uni- and multi-cellular organisms. View Full-Text
Keywords: biosensor; cyanobacteria; Escherichia coli; FRET; 2-oxoglutarate biosensor; cyanobacteria; Escherichia coli; FRET; 2-oxoglutarate

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Chen, H.-L.; Latifi, A.; Zhang, C.-C.; Bernard, C.S. Biosensors-Based In Vivo Quantification of 2-Oxoglutarate in Cyanobacteria and Proteobacteria. Life 2018, 8, 51.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Life EISSN 2075-1729 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top