Wound Healing after Acellular Dermal Substitute Positioning in Dermato-Oncological Surgery: A Prospective Comparative Study
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Design
2.2. Investigational Study Devices
2.3. Study Protocol
2.4. Conventional Histology
2.5. Immunofluorescence
2.6. ELISA Test
2.7. Statistical Analysis
3. Results
3.1. Baseline Patient Characteristics
3.2. Clinical Outcomes
3.3. Neodermis Architecture and Composition
3.4. Cellular Colonization of the ADM
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sex | Age | CV Comorbidities | Histological Dx | Body Site |
---|---|---|---|---|
M | 95 | 0 | SCC | scalp |
F | 82 | 1 | SCC | LL |
F | 87 | 0 | BCC | LL |
M | 90 | 1 | SCC | scalp |
F | 90 | 1 | BCC | scalp |
M | 81 | 1 | SCC | scalp |
M | 84 | 0 | BCC | LL |
M | 89 | 1 | BCC | LL |
M | 58 | 1 | AFX | scalp |
M | 86 | 1 | AFX | scalp |
I | M | Total | ||||||
---|---|---|---|---|---|---|---|---|
N | % | N | % | n | % | p-Value | ||
Infection | N | 5 | 50 | 9 | 90 | 14 | 70 | 0.051 |
Y | 5 | 50 | 1 | 10 | 6 | 30 | ||
Fibronectin (ug/100 uL) | mean ± SD (range) | 0.7 ± 0.3 (0.4–1.2) | 0.8 ± 0.4 (0.4–1.8) | 07 ± 0.4 (0.4–1.8) | 0.716 | |||
Collagen (ug/100 uL) | mean ± SD (range) | 2.0 ± 0.4 (1.3–2.8) | 2.1 ± 0.4 (1.4–2.8) | 2.0 ± 0.4 (1.3–2.8) | 0.737 | |||
ADM persistence | N | 2 | 20 | 7 | 80 | 8 | 40 | 0.004 |
Y | 8 | 80 | 2 | 20 | 10 | 50 | ||
Granulation tissue | immature | 8 | 80 | 4 | 40 | 12 | 60 | 0.046 |
mature | 1 | 10 | 5 | 50 | 6 | 30 | ||
Epithelization | absent | 10 | 100 | 8 | 80 | 18 | 90 | 0.136 |
present | 0 | 0 | 2 | 20 | 2 | 10 | ||
Inflammation | mild | 5 | 50 | 6 | 60 | 11 | 55 | 0.639 |
severe | 4 | 40 | 3 | 30 | 7 | 35 | ||
Granulomatous reaction | N | 6 | 60 | 8 | 80 | 14 | 70 | 0.257 |
Y | 3 | 30 | 1 | 10 | 4 | 20 | ||
Eosinophils | N | 8 | 80 | 7 | 70 | 15 | 75 | 0.527 |
Y | 1 | 10 | 2 | 20 | 3 | 15 | ||
Vascularized area (%) | Mean ± SD (range) | 2.5 ± 1.3 (0.0–4.1) | 2.2 ± 0.6 (0.0–4.1) | 2.3 ± 1.30 (0.0–4.1) | 0.584 | |||
VSS | Mean ± SD (range) | 5.7 ± 1.9 (2–8) | 6.1 ± 1.6 (3–8) | 5.9 ± 1.7 (2–8) | 0.580 |
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Paganelli, A.; Naselli, A.G.; Bertoni, L.; Rossi, E.; Azzoni, P.; Pisciotta, A.; Cesinaro, A.M.; Benassi, L.; Kaleci, S.; Garbarino, F.; et al. Wound Healing after Acellular Dermal Substitute Positioning in Dermato-Oncological Surgery: A Prospective Comparative Study. Life 2023, 13, 463. https://doi.org/10.3390/life13020463
Paganelli A, Naselli AG, Bertoni L, Rossi E, Azzoni P, Pisciotta A, Cesinaro AM, Benassi L, Kaleci S, Garbarino F, et al. Wound Healing after Acellular Dermal Substitute Positioning in Dermato-Oncological Surgery: A Prospective Comparative Study. Life. 2023; 13(2):463. https://doi.org/10.3390/life13020463
Chicago/Turabian StylePaganelli, Alessia, Andrea Giovanni Naselli, Laura Bertoni, Elena Rossi, Paola Azzoni, Alessandra Pisciotta, Anna Maria Cesinaro, Luisa Benassi, Shaniko Kaleci, Federico Garbarino, and et al. 2023. "Wound Healing after Acellular Dermal Substitute Positioning in Dermato-Oncological Surgery: A Prospective Comparative Study" Life 13, no. 2: 463. https://doi.org/10.3390/life13020463