Peer-Review Record
Thermodynamic and Kinetic Sequence Selection in Enzyme-Free Polymer Self-Assembly inside a Non-equilibrium RNA Reactor
Life 2022, 12(4), 567; https://doi.org/10.3390/life12040567
Reviewer 1:
Anonymous
Reviewer 2:
Anonymous
Life 2022, 12(4), 567; https://doi.org/10.3390/life12040567
Submission received: 15 March 2022
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Revised: 3 April 2022
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Accepted: 6 April 2022
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Published: 10 April 2022
(This article belongs to the Special Issue Organic Chemical Evolution regarding the Origin(s) of Life)
Round 1
Reviewer 1 Report
My comments are given in the attached file
Comments for author File: 
Comments.pdf
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
Reviewer 2 Report
Tobias et al. has studied the self-assembly of RNAs via templated ligation inside a non-equilibrium RNA reactor. They have looked the thermodynamic and kinetic sequence selection by modelling the reactor. I recommend to publish this work in Life.
Questions and comments:
- in line 47, the authors talking about from pre-RNA world to RNA world. It is a bit confused me about the author's definition of pre-RNA world. It looks not like the XNA world in this paper. Can authors rephrase this word to make it more clear?
- in line 58-59, The most contribution of RNA/DNA stability should be the hydrogen bond of base pairs not the base stacking. Ref 35 is not a good ref for authors' argument.
- The hydrolysis of RNA giving 2'3'-cycle phosphate, but the ligation in this paper more likely to talking about the 5'-phosphate ligation (like 2-aminoimidazolide phosphate in Jack's work). Thus, the formation and the hydrolysis of RNA is not an equilibrium. It is two irreversible reactions. The authors should clarify it in line 121.
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
