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In Situ 3D-Imaging of the Inner Ear Synapses with a Cochlear Implant

1
Department of Otolaryngology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany
2
Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover, 30559 Hannover, Germany
3
Cluster of Excellence “Hearing4all”, German Research Foundation, DFG (Deutsche Forschungsgemeinschaft), Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Arianna Di Stadio
Life 2021, 11(4), 301; https://doi.org/10.3390/life11040301
Received: 23 February 2021 / Revised: 27 March 2021 / Accepted: 28 March 2021 / Published: 1 April 2021
(This article belongs to the Section Radiobiology, Nuclear Medicine and Medical Imaging)
In recent years sensorineural hearing loss was found to affect not exclusively, nor at first, the sensory cells of the inner ear. The sensory cells’ synapses and subsequent neurites are initially damaged. Auditory synaptopathies also play an important role in cochlear implant (CI) care, as they can lead to a loss of physiological hearing in patients with residual hearing. These auditory synaptopathies and in general the cascades of hearing pathologies have been in the focus of research in recent years with the aim to develop more targeted and individually tailored therapeutics. In the current study, a method to examine implanted inner ears of guinea pigs was developed to examine the synapse level. For this purpose, the cochlea is made transparent and scanned with the implant in situ using confocal laser scanning microscopy. Three different preparation methods were compared to enable both an overview image of the cochlea for assessing the CI position and images of the synapses on the same specimen. The best results were achieved by dissection of the bony capsule of the cochlea. View Full-Text
Keywords: cleared cochlea; ribbon synapse; confocal laser scanning microscopy cleared cochlea; ribbon synapse; confocal laser scanning microscopy
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MDPI and ACS Style

Malfeld, K.; Armbrecht, N.; Volk, H.A.; Lenarz, T.; Scheper, V. In Situ 3D-Imaging of the Inner Ear Synapses with a Cochlear Implant. Life 2021, 11, 301. https://doi.org/10.3390/life11040301

AMA Style

Malfeld K, Armbrecht N, Volk HA, Lenarz T, Scheper V. In Situ 3D-Imaging of the Inner Ear Synapses with a Cochlear Implant. Life. 2021; 11(4):301. https://doi.org/10.3390/life11040301

Chicago/Turabian Style

Malfeld, Kathrin, Nina Armbrecht, Holger A. Volk, Thomas Lenarz, and Verena Scheper. 2021. "In Situ 3D-Imaging of the Inner Ear Synapses with a Cochlear Implant" Life 11, no. 4: 301. https://doi.org/10.3390/life11040301

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