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The Circadian Rhythms of STAT3 in the Rat Pineal Gland and Its Involvement in Arylalkylamine-N-Acetyltransferase Regulation

1
Department of Physiology, Faculty of Science, Charles University, 128 43 Prague, Czech Republic
2
Department of Sleep Medicine and Chronobiology, National Institute of Mental Health, 250 67 Klecany, Czech Republic
3
Laboratory of Pain Research, Institute of Physiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editor: Luca Steardo
Life 2021, 11(10), 1105; https://doi.org/10.3390/life11101105
Received: 15 August 2021 / Revised: 25 September 2021 / Accepted: 27 September 2021 / Published: 18 October 2021
(This article belongs to the Section Physiology and Pathology)
In rodents, the melatonin production by the pineal gland is controlled through adrenergic signaling from the suprachiasmatic nuclei and regulation of the principal enzyme in its synthesis, arylalkylamine-N-acetyltransferase (AANAT). In the present study, we identified increased isoprenaline-induced aa-nat expression and nocturnal AANAT activity in the pineal glands in response to the silencing of the signal transducer and activator of transcription 3 (STAT3) with siRNA or STAT3 inhibitors WP1066 and AZD1480. This AANAT activity enhancement in vivo did not interfere with light-induced AANAT suppression. Systemic or in vitro lipopolysaccharide (LPS) administration markedly increased Stat3 expression and STAT3 phosphorylation, but it did not significantly affect AANAT expression or activity. Simultaneous LPS administration and Stat3 silencing enhanced the aa-nat transcription and AANAT activity to a similar extent as Stat3 inhibition without LPS co-administration. Furthermore, we describe the circadian rhythmicity in Stat3 expression and the phosphorylated form of STAT3 protein in the rat pineal gland. Our data suggest that the higher nocturnal endogenous level of STAT3 in the pineal gland decelerates or hampers the process of NA-induced AANAT activation or affects the AANAT enzyme stability. View Full-Text
Keywords: STAT3; arylalkylamine-N-acetyltransferase; pineal gland; circadian rhythms; lipopolysaccharide; rat STAT3; arylalkylamine-N-acetyltransferase; pineal gland; circadian rhythms; lipopolysaccharide; rat
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MDPI and ACS Style

Moravcová, S.; Filipovská, E.; Spišská, V.; Svobodová, I.; Novotný, J.; Bendová, Z. The Circadian Rhythms of STAT3 in the Rat Pineal Gland and Its Involvement in Arylalkylamine-N-Acetyltransferase Regulation. Life 2021, 11, 1105. https://doi.org/10.3390/life11101105

AMA Style

Moravcová S, Filipovská E, Spišská V, Svobodová I, Novotný J, Bendová Z. The Circadian Rhythms of STAT3 in the Rat Pineal Gland and Its Involvement in Arylalkylamine-N-Acetyltransferase Regulation. Life. 2021; 11(10):1105. https://doi.org/10.3390/life11101105

Chicago/Turabian Style

Moravcová, Simona, Eva Filipovská, Veronika Spišská, Irena Svobodová, Jiří Novotný, and Zdeňka Bendová. 2021. "The Circadian Rhythms of STAT3 in the Rat Pineal Gland and Its Involvement in Arylalkylamine-N-Acetyltransferase Regulation" Life 11, no. 10: 1105. https://doi.org/10.3390/life11101105

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