The Relationship between the Expression of Fatty Acyl Desaturase 2 (fads2) Gene in Peripheral Blood Cells (PBCs) and Liver in Gilthead Seabream, Sparus aurata Broodstock Fed a Low n-3 LC-PUFA Diet

Ferosekhan, Shajahan; Turkmen, Serhat; Xu, Hanlin; Afonso, Juan Manuel; Zamorano, Maria Jesus; Kaushik, Sadasivam; Izquierdo, Marisol

doi:10.3390/life10070117

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The Relationship between the Expression of Fatty Acyl Desaturase 2 (fads2) Gene in Peripheral Blood Cells (PBCs) and Liver in Gilthead Seabream, Sparus aurata Broodstock Fed a Low n-3 LC-PUFA Diet

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Aquaculture Research Group (GIA), IU-ECOAQUA, Universidad de Las Palmas de Gran Canaria, 35214 Telde, Spain

ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar 751002, India

Department of Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA

Author to whom correspondence should be addressed.

Life 2020, 10(7), 117; https://doi.org/10.3390/life10070117

Received: 21 May 2020 / Revised: 10 July 2020 / Accepted: 17 July 2020 / Published: 19 July 2020

(This article belongs to the Section Animal Science)

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Abstract

The principle aim of this study is to elucidate the relationship between the fatty acid desaturase 2 gene (fads2) expression pattern in peripheral blood cells (PBCs) and liver of gilthead seabream (GSB), Sparus aurata broodstock in order to determine the possible use of fads2 expression as a potential biomarker for the selection of broodstock. This selection could be utilized for breeding programs aiming to improve reproduction, health, and nutritional status. Passive Integrated Transponder (PIT)-tagged GSB broodstock (Male-1.22 ± 0.20 kg; 44.8 ± 2 cm and female-2.36 ± 0.64 kg; 55.1 cm) were fed a diet containing low levels of fish meal and fish oil (EPA 2.5; DHA 1.7 and n-3 LC-PUFA 4.6% TFA) for one month. After the feeding period, fads2 expression in PBCs and liver of both male and female broodstock were highly significantly correlated (r = 0.89; p < 0.001). Additionally, in male broodstock, liver fads2 expression was significantly correlated (p < 0.05) to liver contents in 16:0 (r = 0.95; p = 0.04) and total saturates (r = 0.97; p = 0.03) as well as to 20:3n–6/20:2n–6 (r = 0.98; p = 0.02) a Fads2 product/precursor ratio. Overall, we found a positive and significant correlation between fads2 expression levels in the PBCs and liver of GSB broodstock. PBCs fads2 expression levels indicate a strong potential for utilization as a non-invasive method to select animals having increased fatty acid bioconversion capability, better able to deal with diets free of fish meal and fish oil. View Full-Text

Keywords: biomarker; broodstock; fatty acyl desaturase (fads2); fatty acid; lipid metabolism

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Ferosekhan, S.; Turkmen, S.; Xu, H.; Afonso, J.M.; Zamorano, M.J.; Kaushik, S.; Izquierdo, M. The Relationship between the Expression of Fatty Acyl Desaturase 2 (fads2) Gene in Peripheral Blood Cells (PBCs) and Liver in Gilthead Seabream, Sparus aurata Broodstock Fed a Low n-3 LC-PUFA Diet. Life 2020, 10, 117. https://doi.org/10.3390/life10070117

AMA Style

Ferosekhan S, Turkmen S, Xu H, Afonso JM, Zamorano MJ, Kaushik S, Izquierdo M. The Relationship between the Expression of Fatty Acyl Desaturase 2 (fads2) Gene in Peripheral Blood Cells (PBCs) and Liver in Gilthead Seabream, Sparus aurata Broodstock Fed a Low n-3 LC-PUFA Diet. Life. 2020; 10(7):117. https://doi.org/10.3390/life10070117

Chicago/Turabian Style

Ferosekhan, Shajahan, Serhat Turkmen, Hanlin Xu, Juan M. Afonso, Maria J. Zamorano, Sadasivam Kaushik, and Marisol Izquierdo. 2020. "The Relationship between the Expression of Fatty Acyl Desaturase 2 (fads2) Gene in Peripheral Blood Cells (PBCs) and Liver in Gilthead Seabream, Sparus aurata Broodstock Fed a Low n-3 LC-PUFA Diet" Life 10, no. 7: 117. https://doi.org/10.3390/life10070117

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The Relationship between the Expression of Fatty Acyl Desaturase 2 (fads2) Gene in Peripheral Blood Cells (PBCs) and Liver in Gilthead Seabream, Sparus aurata Broodstock Fed a Low n-3 LC-PUFA Diet

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