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Article

The Relationship between the Expression of Fatty Acyl Desaturase 2 (fads2) Gene in Peripheral Blood Cells (PBCs) and Liver in Gilthead Seabream, Sparus aurata Broodstock Fed a Low n-3 LC-PUFA Diet

1
Aquaculture Research Group (GIA), IU-ECOAQUA, Universidad de Las Palmas de Gran Canaria, 35214 Telde, Spain
2
ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar 751002, India
3
Department of Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
*
Author to whom correspondence should be addressed.
Life 2020, 10(7), 117; https://doi.org/10.3390/life10070117
Received: 21 May 2020 / Revised: 10 July 2020 / Accepted: 17 July 2020 / Published: 19 July 2020
(This article belongs to the Section Animal Science)
The principle aim of this study is to elucidate the relationship between the fatty acid desaturase 2 gene (fads2) expression pattern in peripheral blood cells (PBCs) and liver of gilthead seabream (GSB), Sparus aurata broodstock in order to determine the possible use of fads2 expression as a potential biomarker for the selection of broodstock. This selection could be utilized for breeding programs aiming to improve reproduction, health, and nutritional status. Passive Integrated Transponder (PIT)-tagged GSB broodstock (Male-1.22 ± 0.20 kg; 44.8 ± 2 cm and female-2.36 ± 0.64 kg; 55.1 cm) were fed a diet containing low levels of fish meal and fish oil (EPA 2.5; DHA 1.7 and n-3 LC-PUFA 4.6% TFA) for one month. After the feeding period, fads2 expression in PBCs and liver of both male and female broodstock were highly significantly correlated (r = 0.89; p < 0.001). Additionally, in male broodstock, liver fads2 expression was significantly correlated (p < 0.05) to liver contents in 16:0 (r = 0.95; p = 0.04) and total saturates (r = 0.97; p = 0.03) as well as to 20:3n–6/20:2n–6 (r = 0.98; p = 0.02) a Fads2 product/precursor ratio. Overall, we found a positive and significant correlation between fads2 expression levels in the PBCs and liver of GSB broodstock. PBCs fads2 expression levels indicate a strong potential for utilization as a non-invasive method to select animals having increased fatty acid bioconversion capability, better able to deal with diets free of fish meal and fish oil. View Full-Text
Keywords: biomarker; broodstock; fatty acyl desaturase (fads2); fatty acid; lipid metabolism biomarker; broodstock; fatty acyl desaturase (fads2); fatty acid; lipid metabolism
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MDPI and ACS Style

Ferosekhan, S.; Turkmen, S.; Xu, H.; Afonso, J.M.; Zamorano, M.J.; Kaushik, S.; Izquierdo, M. The Relationship between the Expression of Fatty Acyl Desaturase 2 (fads2) Gene in Peripheral Blood Cells (PBCs) and Liver in Gilthead Seabream, Sparus aurata Broodstock Fed a Low n-3 LC-PUFA Diet. Life 2020, 10, 117. https://doi.org/10.3390/life10070117

AMA Style

Ferosekhan S, Turkmen S, Xu H, Afonso JM, Zamorano MJ, Kaushik S, Izquierdo M. The Relationship between the Expression of Fatty Acyl Desaturase 2 (fads2) Gene in Peripheral Blood Cells (PBCs) and Liver in Gilthead Seabream, Sparus aurata Broodstock Fed a Low n-3 LC-PUFA Diet. Life. 2020; 10(7):117. https://doi.org/10.3390/life10070117

Chicago/Turabian Style

Ferosekhan, Shajahan, Serhat Turkmen, Hanlin Xu, Juan M. Afonso, Maria J. Zamorano, Sadasivam Kaushik, and Marisol Izquierdo. 2020. "The Relationship between the Expression of Fatty Acyl Desaturase 2 (fads2) Gene in Peripheral Blood Cells (PBCs) and Liver in Gilthead Seabream, Sparus aurata Broodstock Fed a Low n-3 LC-PUFA Diet" Life 10, no. 7: 117. https://doi.org/10.3390/life10070117

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