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Antibodies 2016, 5(2), 7;

Development and Characterization of New Species Cross-Reactive Anti-Sialoadhesin Monoclonal Antibodies

Laboratory of Microbiology, Parasitology and Hygiene, University of Antwerp (UA), Antwerp 2610, Belgium
Laboratory of Virology, Ghent University, Merelbeke 9820, Belgium
Inflammation Research Center, VIB-Department of Internal Medicine, Ghent University, Ghent 9000, Belgium
Laboratory of Cell Biology and Histology, University of Antwerp (UA), Antwerp 2020, Belgium
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: Mark Cragg
Received: 25 January 2016 / Revised: 3 March 2016 / Accepted: 14 March 2016 / Published: 23 March 2016
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Sialoadhesin (Sn) is a surface receptor expressed on a subset of macrophages in steady state conditions. During inflammation and diseases, Sn is highly upregulated on macrophages and blood monocytes. Therefore, therapies using monoclonal antibodies (mAbs) to target Sn-positive (Sn+) cells are a potential strategy for targeted treatment. It has been shown that Sn internalizes after binding with a mAb, though it is not clear whether this is species-specific. In this study, new Sn-specific mAbs were developed and analyzed for cross-reactivity between species. In addition, the newly developed mAbs were compared to mAbs used in previous research for their epitope recognition and other Sn-specific characteristics. Both species-specific and cross-reactive antibodies could be identified. Furthermore, sialic acid-binding of red blood cells (RBC) could be inhibited with mAbs recognizing different epitopes and all mAb showed internalization of Sn. The newly developed mAbs can be used as novel tools for Sn research and further analysis of Sn internalization in different species. View Full-Text
Keywords: sialoadhesin; monoclonal antibody; internalization sialoadhesin; monoclonal antibody; internalization

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De Schryver, M.; Van Gorp, H.; Hoebeke, I.; De Maeyer, B.; Ooms, K.; Pintelon, I.; Maes, L.J.; Cos, P.; Nauwynck, H.J.; Delputte, P.L. Development and Characterization of New Species Cross-Reactive Anti-Sialoadhesin Monoclonal Antibodies. Antibodies 2016, 5, 7.

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