Binding Analysis of Human Immunoglobulin G as a Zinc-Binding Protein
Abstract
:1. Introduction
2. Results and Discussion
2.1. Binding of Mammalian IgGs to Zn-Beads
2.2. Binding Mechanism of Human IgG to Zn-Beads
2.3. Binding of Human IgG and Fibrinogen to Zinc Ions
3. Experimental Section
3.1. Chemicals
3.2. SDS-PAGE
3.3. Binding of Mammalian IgGs to Zinc Ion–Immobilized Beads
3.4. Comparison of Binding Affinity of Human Fibrinogen and IgG to Zn-Beads
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of interest
References
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Yamanaka, Y.; Matsugano, S.; Yoshikawa, Y.; Orino, K. Binding Analysis of Human Immunoglobulin G as a Zinc-Binding Protein. Antibodies 2016, 5, 13. https://doi.org/10.3390/antib5020013
Yamanaka Y, Matsugano S, Yoshikawa Y, Orino K. Binding Analysis of Human Immunoglobulin G as a Zinc-Binding Protein. Antibodies. 2016; 5(2):13. https://doi.org/10.3390/antib5020013
Chicago/Turabian StyleYamanaka, Yu, Sho Matsugano, Yasunaga Yoshikawa, and Koichi Orino. 2016. "Binding Analysis of Human Immunoglobulin G as a Zinc-Binding Protein" Antibodies 5, no. 2: 13. https://doi.org/10.3390/antib5020013
APA StyleYamanaka, Y., Matsugano, S., Yoshikawa, Y., & Orino, K. (2016). Binding Analysis of Human Immunoglobulin G as a Zinc-Binding Protein. Antibodies, 5(2), 13. https://doi.org/10.3390/antib5020013