Next Article in Journal / Special Issue
Preparation of Knockout Extract by Immunoaffinity Column and Its Application
Previous Article in Journal / Special Issue
Immunochemical Analysis of the Antimalarial Drugs Artemisinin and Artesunate
Article Menu

Export Article

Open AccessArticle
Antibodies 2012, 1(3), 284-293;

Development of Eastern Blotting Technique for Analysis of Baicalin Using Anti-Baicalin Monoclonal Antibody

Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859-3298, Japan
Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand
Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
Author to whom correspondence should be addressed.
Received: 31 August 2012 / Revised: 18 November 2012 / Accepted: 22 November 2012 / Published: 27 November 2012
Full-Text   |   PDF [634 KB, uploaded 27 November 2012]   |  


Scutellariae radix (S. radix) is one of the most important crude drugs used in Kampo medicines (KMs). A part of its pharmaceutical properties is due to flavone glycoside, baicalin (BI). A technique named eastern blotting was developed for the specific and easy identification of BI in the extracts of crude drugs and KMs using anti-BI monoclonal antibody (MAb). BI separated by silica gel thin-layer chromatography (TLC) transferred to a polyethersulfone (PES) membrane was treated with a NaIO4 solution and reacted with bovine serum albumin (BSA) preparing BI-BSA conjugate on the PES membrane. Anti-BI MAb was bound and then antibody labeled with peroxidase directed against anti-BI MAb. Finally, a substrate was added and then BI was detected. As little as 1 mg of BI was still detected on the PES membrane under immunostaining method. Various samples of S. radix and KMs which contain S. radix were qualitatively analyzed, and BI was visually detected by eastern blotting technique. Furthermore, this method was applied for the immunohistochemical study to investigate the distribution of BI in the fresh root of Scutellaria baicalensis using immunoblotting by transferred from fresh root to the PES membrane. View Full-Text
Keywords: baicalin; monoclonal antibody; eastern blotting; Scutellaria radix baicalin; monoclonal antibody; eastern blotting; Scutellaria radix

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

MDPI and ACS Style

Morinaga, O.; Mukae, R.; Uto, T.; Pariyawongsakul, Y.; Putalun, W.; Tanaka, H.; Shoyama, Y. Development of Eastern Blotting Technique for Analysis of Baicalin Using Anti-Baicalin Monoclonal Antibody. Antibodies 2012, 1, 284-293.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Antibodies EISSN 2073-4468 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top